Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,321,941	C→T	A336T (GCC→ACC)	trpE ←	anthranilate synthase subunit TrpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,321,941	0	C	T	100.0%	300.0 / NA	85	A336T (GCC→ACC)	trpE	anthranilate synthase subunit TrpE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/40);  total (45/40)

CCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTATCCTGCATAAAAAACATGTACGG  >  NC_000913/1321808‑1322076                                                                                                                                      |                                                                                                                                        ccGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGcgacg                                                                                                                                   >  2:112338/1‑140 (MQ=255)  cGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGcgacga                                                                                                                                  <  1:539759/140‑1 (MQ=255)    ggggTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGAcc                                                                                                                                <  1:1014008/140‑1 (MQ=255)                  tGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTc                                                                                                                  >  2:830949/1‑140 (MQ=255)                  tGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTc                                                                                                                  >  1:526624/1‑140 (MQ=255)                  tGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTc                                                                                                                  >  1:738230/1‑140 (MQ=255)                       gATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCa                                                                                                             >  2:150986/1‑140 (MQ=255)                       gATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCa                                                                                                             >  2:961575/1‑140 (MQ=255)                             tACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCggg                                                                                                       >  2:372695/1‑140 (MQ=255)                             tACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCggg                                                                                                       >  2:59137/1‑140 (MQ=255)                             tACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCggg                                                                                                       >  1:443412/1‑140 (MQ=255)                              aCGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGt                                                                                                      <  2:443412/140‑1 (MQ=255)                              aCGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGt                                                                                                      <  2:526624/140‑1 (MQ=255)                               cGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGt                                                                                                      >  2:170232/1‑139 (MQ=255)                               cGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGt                                                                                                      <  1:170232/139‑1 (MQ=255)                                  gCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGAt                                                                                                  >  1:617144/1‑140 (MQ=255)                                  gCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGAt                                                                                                  >  2:387781/1‑140 (MQ=255)                                  gCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGAt                                                                                                  >  2:236572/1‑140 (MQ=255)                                       aTCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCaa                                                                                             >  2:210776/1‑140 (MQ=255)                                       aTCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCaa                                                                                             >  1:68891/1‑140 (MQ=255)                                        tCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAAt                                                                                            <  1:387781/140‑1 (MQ=255)                                             cAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTggc                                                                                       <  1:59137/140‑1 (MQ=255)                                               gCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTggcgg                                                                                     <  2:409350/140‑1 (MQ=255)                                                cATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTggcggc                                                                                    <  2:292356/140‑1 (MQ=255)                                                 aTCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCt                                                                                   <  1:961575/140‑1 (MQ=255)                                                 aTCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCt                                                                                   >  2:254593/1‑140 (MQ=255)                                                 aTCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCt                                                                                   <  1:150986/140‑1 (MQ=255)                                                    aGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCGGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCtggt                                                                                >  1:674662/1‑140 (MQ=255)                                                    aGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCtggt                                                                                <  1:254593/140‑1 (MQ=255)                                                           cAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcatca                                                                         <  1:112338/140‑1 (MQ=255)                                                            aGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGcatcat                                                                        <  2:68891/140‑1 (MQ=255)                                                                   tctTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTgag                                                                 >  1:843054/1‑140 (MQ=255)                                                                     tttATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTgagcg                                                               >  2:55838/1‑140 (MQ=255)                                                                       tATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTgagcgag                                                             <  2:674662/140‑1 (MQ=255)                                                                            tCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTc                                                        >  2:57432/1‑140 (MQ=255)                                                                            tCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTc                                                        >  1:806998/1‑140 (MQ=255)                                                                               gTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTgagc                                                                >  1:129876/1‑129 (MQ=255)                                                                               gTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTgagc                                                                <  2:129876/129‑1 (MQ=255)                                                                               gTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCgg                                                     >  1:102040/1‑140 (MQ=255)                                                                               gTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCgg                                                     >  1:96480/1‑140 (MQ=255)                                                                               gTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCgg                                                     >  1:434108/1‑140 (MQ=255)                                                                               gTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATAGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCgg                                                     >  2:804426/1‑140 (MQ=255)                                                                                 aCGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCg                                                   <  2:102040/140‑1 (MQ=255)                                                                                 aCGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCg                                                   <  1:210776/140‑1 (MQ=255)                                                                                 aCGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCg                                                   <  2:617144/140‑1 (MQ=255)                                                                                       ttCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACgcgc                                             >  2:284693/1‑140 (MQ=255)                                                                                        tCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGcc                                            >  2:104911/1‑140 (MQ=255)                                                                                        tCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGcc                                            <  1:372695/140‑1 (MQ=255)                                                                                          cAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCaa                                          >  1:822519/1‑140 (MQ=255)                                                                                             ttCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATa                                       <  2:738230/140‑1 (MQ=255)                                                                                               cAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAgg                                     >  2:784172/1‑140 (MQ=255)                                                                                                 aTACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGt                                   >  2:971636/1‑140 (MQ=255)                                                                                                 aTACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGt                                   <  1:608022/140‑1 (MQ=255)                                                                                                 aTACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGt                                   >  2:608022/1‑140 (MQ=255)                                                                                                 aTACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGt                                   <  2:843054/140‑1 (MQ=255)                                                                                                   aCGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGa                                 <  1:236572/140‑1 (MQ=255)                                                                                                   aCGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGa                                 <  2:822519/140‑1 (MQ=255)                                                                                                     ggCTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGaaa                               <  1:784172/140‑1 (MQ=255)                                                                                                       cTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATc                             >  2:757519/1‑140 (MQ=255)                                                                                                       cTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATc                             >  2:145677/1‑140 (MQ=255)                                                                                                       cTGTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTAGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATc                             >  2:910989/1‑140 (MQ=255)                                                                                                         gTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGt                                   <  2:639627/132‑1 (MQ=255)                                                                                                         gTCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGt                                   >  1:639627/1‑132 (MQ=255)                                                                                                          tCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGa                                 >  1:88113/1‑133 (MQ=255)                                                                                                          tCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGa                                 <  2:88113/133‑1 (MQ=255)                                                                                                          tCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGa                                 <  2:914540/133‑1 (MQ=255)                                                                                                          tCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGa                                 >  1:914540/1‑133 (MQ=255)                                                                                                          tCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGa                                 <  2:683002/133‑1 (MQ=255)                                                                                                          tCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGa                                 >  1:683002/1‑133 (MQ=255)                                                                                                          tCGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCAtt                          >  1:427132/1‑140 (MQ=255)                                                                                                           cGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTa                         <  2:434108/140‑1 (MQ=255)                                                                                                           cGAGATCTCTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTa                         <  2:96480/140‑1 (MQ=255)                                                                                                                tctcTGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATAGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTATCCTg                    <  1:804426/140‑1 (MQ=255)                                                                                                                    tGTCCAGTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTATCCTGCATa                <  1:104911/140‑1 (MQ=255)                                                                                                                          gTGAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTATCCTGCATAAAAAAc          <  2:427132/140‑1 (MQ=255)                                                                                                                            gAACCATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATATGGTGAAATCATTATCCTGCATAAAAAAGAt        >  2:624597/1‑140 (MQ=255)                                                                                                                               ccATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTATCCTGCATAAAAAACATGTa     <  1:145677/140‑1 (MQ=255)                                                                                                                               ccATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTATCCTGCATAAAAAACATGTa     <  1:910989/140‑1 (MQ=255)                                                                                                                               ccATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTATCCTGCATAAAAAACATGTa     <  1:55838/140‑1 (MQ=255)                                                                                                                                cATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTATCCt                     >  1:249275/1‑123 (MQ=255)                                                                                                                                cATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTATCCt                     <  2:249275/123‑1 (MQ=255)                                                                                                                                cATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTATCCTGCATAAAAAACATGt      >  1:947995/1‑138 (MQ=255)                                                                                                                                cATCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTATCCTGCATAAAAAACATGt      <  2:947995/138‑1 (MQ=255)                                                                                                                                  tCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTATCCTGCATAAAAAACATGTACgg  >  2:455687/1‑140 (MQ=255)                                                                                                                                  tCGGTGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTATCCTGCATAAAAAACATGTACgg  >  2:441717/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        CCGGGGGTGCAAATGCGTGCCAGATCATTACGGGCGAGATCAACCAGCATCAGATGTTCAGACAGCTCTTTATGATCGGTACGCATTTCCAGTTCAATACGGCTGTCGAGATCTCTGTCCAGTGAACCATCGGCGCGACGACCGCGTGGGCGTGTTCCGGCAATCGGGTAGATCTCAATCTGGCGGCTGGTGGCATCATACTTGAGCGAGCTTTCCGGCGACGCGCCAAATAGGGTGAAATCATTATCCTGCATAAAAAACATGTACGG  >  NC_000913/1321808‑1322076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑