Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,389,344	A→G	T139A (ACA→GCA)	ycjG →	L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,389,344	0	A	G	100.0%	183.0 / NA	52	T139A (ACA→GCA)	ycjG	L‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/29);  total (23/29)

TGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGA  >  NC_000913/1389208‑1389480                                                                                                                                         |                                                                                                                                         tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAgcac                                                                                                                                       >  1:770600/1‑140 (MQ=255)          ggATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTggcagg                                                                                                                              <  1:381861/140‑1 (MQ=255)           gATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAgca                                                                                                                                        >  2:310637/1‑129 (MQ=255)           gATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAgca                                                                                                                                        <  1:310637/129‑1 (MQ=255)           gATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTggcaggc                                                                                                                             >  2:849350/1‑140 (MQ=255)           gATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTggcaggc                                                                                                                             >  2:717007/1‑140 (MQ=255)                ggCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGgcg                                                                                                                        >  1:883206/1‑140 (MQ=255)                  ccGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGcgcg                                                                                                                      <  1:191434/140‑1 (MQ=255)                  ccGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGcgcg                                                                                                                      <  2:770600/140‑1 (MQ=255)                               gcagcaATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAg                                                                                                         <  1:212061/140‑1 (MQ=255)                                 agcaATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTg                                                                                                       >  1:561892/1‑140 (MQ=255)                                   caATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGaa                                                                                                     <  2:546224/140‑1 (MQ=255)                                    aaTCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAg                                                                                                    <  2:277334/140‑1 (MQ=255)                                     aTCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGa                                                                                                      <  1:501204/137‑1 (MQ=255)                                     aTCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGc                                                                                                   >  2:568862/1‑140 (MQ=255)                                     aTCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGc                                                                                                   >  2:743719/1‑140 (MQ=255)                                     aTCGCTGGCTGATTTGATCCGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGACATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGa                                                                                                      >  2:501204/1‑137 (MQ=255)                                       cGCTGGCTGATTTGATCGGTATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTg                                                                                                 <  1:717007/140‑1 (MQ=255)                                         ctggctgATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGa                                                                                               <  1:739235/140‑1 (MQ=255)                                           ggctgATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATa                                                                                             <  1:259172/140‑1 (MQ=255)                                            gctgATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGa                                                                                               >  2:485500/1‑137 (MQ=255)                                            gctgATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGa                                                                                               <  1:485500/137‑1 (MQ=255)                                             ctgATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAAc                                                                                           >  2:347784/1‑140 (MQ=255)                                                            tAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAg                                                                            <  1:568660/140‑1 (MQ=255)                                                             aaCGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGc                                                                           <  1:620648/140‑1 (MQ=255)                                                              aCGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCg                                                                          <  1:568862/140‑1 (MQ=255)                                                              aCGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCg                                                                          <  1:743719/140‑1 (MQ=255)                                                                    cccGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGAtggt                                                                    >  1:770096/1‑140 (MQ=255)                                                                          aCAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAAt                                                              >  2:252896/1‑140 (MQ=255)                                                                               tATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGca                                                         <  1:849350/140‑1 (MQ=255)                                                                                      gCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAAt                                                              >  1:251168/1‑128 (MQ=255)                                                                                      gCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAAt                                                              <  2:251168/128‑1 (MQ=255)                                                                                      gCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGca                                                         <  1:823152/133‑1 (MQ=255)                                                                                      gCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGca                                                         >  2:823152/1‑133 (MQ=255)                                                                                      gCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCtgt                                                  >  2:929788/1‑140 (MQ=255)                                                                                        acaGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCa                                                                >  1:667853/1‑124 (MQ=255)                                                                                        acaGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCa                                                                <  2:667853/124‑1 (MQ=255)                                                                                            aCGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGa                                            >  1:998546/1‑140 (MQ=255)                                                                                             cGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGcc                                               <  1:974404/136‑1 (MQ=255)                                                                                             cGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGcc                                               >  2:974404/1‑136 (MQ=255)                                                                                                ttGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCg                                        <  1:252896/140‑1 (MQ=255)                                                                                                          tACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCg                              >  1:501604/1‑140 (MQ=255)                                                                                                          tACGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCg                              <  1:347784/140‑1 (MQ=255)                                                                                                            cGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGt                             <  1:657342/139‑1 (MQ=255)                                                                                                            cGCCTGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGt                             >  2:657342/1‑139 (MQ=255)                                                                                                                tGATCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATg                        <  2:998546/140‑1 (MQ=255)                                                                                                                   tCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTg                                  >  2:386731/1‑127 (MQ=255)                                                                                                                   tCAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTg                                  <  1:386731/127‑1 (MQ=255)                                                                                                                    cAGATGGCCAATAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCaaa                    >  2:309962/1‑140 (MQ=255)                                                                                                                              aTAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTgg          <  2:501604/140‑1 (MQ=255)                                                                                                                               tAGTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGc         <  1:309962/140‑1 (MQ=255)                                                                                                                                 gTGCATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGt       >  1:131514/1‑140 (MQ=255)                                                                                                                                    cATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCaa                     >  2:873220/1‑123 (MQ=255)                                                                                                                                    cATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCaa                     <  1:873220/123‑1 (MQ=255)                                                                                                                                    cATCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCa    >  2:781648/1‑140 (MQ=255)                                                                                                                                      tCAGCACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGa  <  2:770096/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGAAATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGA  >  NC_000913/1389208‑1389480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑