Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,488,705	C→T	L158L (CTC→CTT)	aldA →	aldehyde dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,488,705	0	C	T	100.0%	193.8 / NA	55	L158L (CTC→CTT)	aldA	aldehyde dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/27);  total (28/27)

ATTACATGGCGGAGTGGGCACGGCGTTACGAGGGCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGTTTAACC  >  NC_000913/1488569‑1488841                                                                                                                                         |                                                                                                                                         aTTACATGGCGGAGTGGGCACGGCGTTACGAGGGCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTAtt                                                                                                                                       >  1:191199/1‑140 (MQ=255) aTTACATGGCGGAGTGGGCACGGCGTTACGAGGGCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTAtt                                                                                                                                       >  2:53351/1‑140 (MQ=255)      aTGGCGGAGTGGGCACGGCGTTACGAGGGCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCg                                                                                                                                  >  2:932588/1‑140 (MQ=255)        ggCGGAGTGGGCACGGCGTTACGAGGGCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCa                                                                                                                                >  1:982511/1‑140 (MQ=255)          cGGAGTGGGCACGGCGTTACTAGGGCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCaaa                                                                                                                              >  1:39167/1‑140 (MQ=255)          cGGAGTGGGCACGGCGTTACGAGGGCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCaaa                                                                                                                              >  1:790053/1‑140 (MQ=255)            gAGTGGGCACGGCGTTACGAGGGCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAAt                                                                                                                            <  2:790053/140‑1 (MQ=255)               tGGGCACGGCGTTACGAGGGCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGc                                                                                                                         >  1:728759/1‑140 (MQ=255)                gggCACGGCGTTACGAGGGCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCt                                                                                                                        >  2:312667/1‑140 (MQ=255)                gggCACGGCGTTACGAGGGCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCt                                                                                                                        >  1:499776/1‑140 (MQ=255)                             cGAGGGCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGa                                                                                                           <  2:513358/140‑1 (MQ=255)                             cGAGGGCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGa                                                                                                           <  2:163326/140‑1 (MQ=255)                             cGAGGGCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGa                                                                                                           <  2:448180/140‑1 (MQ=255)                                  gCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTTACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCg                                                                                                        >  2:351414/1‑138 (MQ=255)                                  gCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTTACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCg                                                                                                        <  1:351414/138‑1 (MQ=255)                                  gCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGt                                                                                                      >  2:765691/1‑140 (MQ=255)                                    gagaTTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGAc                                                                                                          >  2:944238/1‑134 (MQ=255)                                    gagaTTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGAc                                                                                                          <  1:944238/134‑1 (MQ=255)                                          attCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCAt                                                                                              <  2:499776/140‑1 (MQ=255)                                          attCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCAt                                                                                              <  1:378642/140‑1 (MQ=255)                                           ttCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATc                                                                                             <  1:312667/140‑1 (MQ=255)                                            tCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGAc                                                                                                          <  1:358876/126‑1 (MQ=255)                                            tCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGAc                                                                                                          >  2:358876/1‑126 (MQ=255)                                                aGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCAt                                                                                              >  2:391173/1‑134 (MQ=255)                                                aGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCAt                                                                                              <  1:391173/134‑1 (MQ=255)                                                         ccAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAg                                                                               <  2:728759/140‑1 (MQ=255)                                                                aaaaTATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAAttt                                                                        >  1:496096/1‑140 (MQ=255)                                                                  aaTATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTAc                                                                      >  1:642181/1‑140 (MQ=255)                                                                  aaTATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTAc                                                                      >  2:27611/1‑140 (MQ=255)                                                                   atatTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACg                                                                     >  1:296334/1‑140 (MQ=255)                                                                      ttCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCa                                                                  <  2:496096/140‑1 (MQ=255)                                                                       tCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCaa                                                                 >  1:990300/1‑140 (MQ=255)                                                                           ttGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACaa                                                             <  1:765691/140‑1 (MQ=255)                                                                              tttAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGc                                                          >  1:230857/1‑140 (MQ=255)                                                                                     gTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCa                                                   <  1:276667/140‑1 (MQ=255)                                                                                                   aCTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAAtcgt                                     >  1:420444/1‑140 (MQ=255)                                                                                                     tACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAAtcgtcg                                   <  1:932588/140‑1 (MQ=255)                                                                                                     tACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAAtcgtcg                                   >  2:524875/1‑140 (MQ=255)                                                                                                     tACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAAtcgt                                     <  2:383152/138‑1 (MQ=255)                                                                                                     tACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAAtcgt                                     >  1:383152/1‑138 (MQ=255)                                                                                                      aCCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGa                                  <  2:990300/140‑1 (MQ=255)                                                                                                        cGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATg                                >  2:4390/1‑140 (MQ=255)                                                                                                         ggCATTCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGa                               >  2:638982/1‑140 (MQ=255)                                                                                                              tCTGCCGTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAg                          >  2:63543/1‑140 (MQ=255)                                                                                                                    gTGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTc                    <  1:63543/140‑1 (MQ=255)                                                                                                                     tGGAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTcc                   >  2:324965/1‑140 (MQ=255)                                                                                                                      ggAACTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCcg                  <  1:27611/140‑1 (MQ=255)                                                                                                                          cTTCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCcgcgcg              <  1:558095/140‑1 (MQ=255)                                                                                                                            tCCCGTTCTTCCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCgcggc            <  1:4390/140‑1 (MQ=255)                                                                                                                                 ttcttcCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGtt       <  1:572760/140‑1 (MQ=255)                                                                                                                                 ttcttcCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGtt       <  2:39167/140‑1 (MQ=255)                                                                                                                                  tcttcCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGttt      <  1:638982/140‑1 (MQ=255)                                                                                                                                  tcttcCTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGttt      >  2:137222/1‑140 (MQ=255)                                                                                                                                      ccTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGTTTAAcc  <  2:420444/140‑1 (MQ=255)                                                                                                                                      ccTTATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGTTTAAcc  <  2:296334/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         ATTACATGGCGGAGTGGGCACGGCGTTACGAGGGCGAGATTATTCAAAGCGATCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGTTTAACC  >  NC_000913/1488569‑1488841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑