Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,515,011	A→G	T84A (ACG→GCG)	ydcV →	putative ABC transporter membrane subunit YdcV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,515,011	0	A	G	100.0%	235.6 / NA	66	T84A (ACG→GCG)	ydcV	putative ABC transporter membrane subunit YdcV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/34);  total (32/34)

TTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCAT  >  NC_000913/1514879‑1515141                                                                                                                                     |                                                                                                                                   tttAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGc                                                                                                                             <  1:373954/140‑1 (MQ=255)         tCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCcg                                                                                                                     >  2:997350/1‑140 (MQ=255)           cACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGAGTTATGGGCGCTGGCAGCTGCcggg                                                                                                                   >  1:504762/1‑138 (MQ=255)               gCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCtgt                                                                                                               >  1:728898/1‑140 (MQ=255)                   ggCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTATCACTTAAAGTGGCGGCGCTGGCGCCATTAATTGCGCTGGGGTTAGGGGCGCTGGCAGCTGCCGCGCTgcggcg                                                                                                           >  2:894800/1‑140 (MQ=255)                             tgcgctgGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACttt                                                                                                 >  2:882568/1‑140 (MQ=255)                               cgctgGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACttttt                                                                                               >  2:47974/1‑140 (MQ=255)                                              gcagcaCAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCAt                                                                                >  2:644664/1‑140 (MQ=255)                                              gcagcaCAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCAt                                                                                >  2:601605/1‑140 (MQ=255)                                              gcagcaCAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCAt                                                                                >  2:384104/1‑140 (MQ=255)                                                    caGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCt                                                                          >  1:301766/1‑140 (MQ=255)                                                    caGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCt                                                                          <  2:198255/140‑1 (MQ=255)                                                       cGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGtt                                                                       >  2:34653/1‑140 (MQ=255)                                                         tAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCt                                                                          >  2:1011694/1‑135 (MQ=255)                                                         tAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCt                                                                          <  1:1011694/135‑1 (MQ=255)                                                         tAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTAc                                                                     <  2:301766/140‑1 (MQ=255)                                                         tAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTAc                                                                     <  1:997350/140‑1 (MQ=255)                                                             gATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTActgct                                                                 <  1:974619/140‑1 (MQ=255)                                                              atatTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTActgctg                                                                <  1:356814/140‑1 (MQ=255)                                                              atatTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTActgctg                                                                <  1:601605/140‑1 (MQ=255)                                                              atatTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTActgctg                                                                <  2:728898/140‑1 (MQ=255)                                                               tatTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTActgctgc                                                               >  2:943199/1‑140 (MQ=255)                                                                      gATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCAt                                                        >  2:790241/1‑140 (MQ=255)                                                                         gCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTAc                                                                     <  1:212813/124‑1 (MQ=255)                                                                         gCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTAc                                                                     >  2:212813/1‑124 (MQ=255)                                                                         gCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTgc                                                     >  1:510001/1‑140 (MQ=255)                                                                           cGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTgcgc                                                   >  2:602882/1‑140 (MQ=255)                                                                           cGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTgcg                                                    >  1:282598/1‑139 (MQ=255)                                                                           cGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTgcg                                                    <  2:282598/139‑1 (MQ=255)                                                                           cGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGGGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTgcgc                                                   >  1:384126/1‑140 (MQ=255)                                                                             tGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTg                                                 >  1:286183/1‑140 (MQ=255)                                                                             tGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTg                                                 <  1:894800/140‑1 (MQ=255)                                                                              gACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGc                                                <  1:943199/140‑1 (MQ=255)                                                                              gACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGc                                                <  1:644664/140‑1 (MQ=255)                                                                                    gTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCa                                          <  1:790241/140‑1 (MQ=255)                                                                                    gTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCa                                          <  1:34653/140‑1 (MQ=255)                                                                                     tCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCAt                                         >  1:337939/1‑140 (MQ=255)                                                                                     tCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCAt                                         >  2:403952/1‑140 (MQ=255)                                                                                     tCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCAt                                         >  2:33651/1‑140 (MQ=255)                                                                                        cTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGc                                           <  2:2162/135‑1 (MQ=255)                                                                                        cTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGc                                           >  1:2162/1‑135 (MQ=255)                                                                                          tAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCa                                    <  2:286183/140‑1 (MQ=255)                                                                                          tAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCa                                    <  1:33651/140‑1 (MQ=255)                                                                                          tAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCa                                    <  1:312450/140‑1 (MQ=255)                                                                                           aaaGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCg                                              <  2:56362/129‑1 (MQ=255)                                                                                           aaaGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCg                                              >  1:56362/1‑129 (MQ=255)                                                                                           aaaGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCAc                                   <  1:403952/140‑1 (MQ=255)                                                                                            aaGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACt                                  >  2:290002/1‑140 (MQ=255)                                                                                             aGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTg                                 >  1:850368/1‑140 (MQ=255)                                                                                               tGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCAtt                                        <  2:494054/131‑1 (MQ=255)                                                                                               tGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCAtt                                        >  1:494054/1‑131 (MQ=255)                                                                                               tGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCa                                    <  1:635233/135‑1 (MQ=255)                                                                                               tGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCa                                    >  2:635233/1‑135 (MQ=255)                                                                                                ggcggcGCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTc                              <  2:337939/140‑1 (MQ=255)                                                                                                 gcggcgCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCt                             >  2:686917/1‑140 (MQ=255)                                                                                                  cggcgCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTg                            <  1:979168/140‑1 (MQ=255)                                                                                                   ggcgCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTgg                           <  2:850368/140‑1 (MQ=255)                                                                                                   ggcgCTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTgg                           >  1:747625/1‑140 (MQ=255)                                                                                                      gcTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGt                        <  2:151552/140‑1 (MQ=255)                                                                                                      gcTGGCGACATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGt                        <  2:747625/140‑1 (MQ=255)                                                                                                              cATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAAcc                <  1:47974/140‑1 (MQ=255)                                                                                                              cATTAATTGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAAcc                <  1:882568/140‑1 (MQ=255)                                                                                                                    ttGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGttat                     <  2:636116/129‑1 (MQ=255)                                                                                                                    ttGCGCTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGttat                     >  1:636116/1‑129 (MQ=255)                                                                                                                      gcgcTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTaa        <  2:327209/140‑1 (MQ=255)                                                                                                                         cTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAAc     >  2:515148/1‑140 (MQ=255)                                                                                                                         cTGGTGTTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAAc     >  1:733484/1‑140 (MQ=255)                                                                                                                            gtgtTAGGGGCGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCAt  <  2:384126/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   TTTAGTTTTCCACCGCAGGGCCTGACGCTGCGCTGGTTTAGCGTGGCAGCACAGCGTAGTGATATTCTTGATGCCGTGACACTGTCACTTAAAGTGGCGGCGCTGGCGACATTAATTGCGCTGGTGTTAGGGACGCTGGCAGCTGCCGCGCTGTGGCGACGAGACTTTTTCGGCAAAAACGCCATTTCGCTGTTACTGCTGCTGCCCATTGCGCTGCCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCAT  >  NC_000913/1514879‑1515141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑