Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,614,810	C→T	L3L (CTG→TTG)	yneJ →	putative LysR‑type DNA‑binding transcriptional regulator YneJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,614,810	0	C	T	100.0%	197.1 / NA	56	L3L (CTG→TTG)	yneJ	putative LysR‑type DNA‑binding transcriptional regulator YneJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/26);  total (30/26)

ATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGT  >  NC_000913/1614675‑1614945                                                                                                                                        |                                                                                                                                        aTTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGAc                                                                                                                                     >  1:113640/1‑140 (MQ=255)      aTGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAAc                                                                                                                                <  1:976326/140‑1 (MQ=255)        gAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTg                                                                                                                              <  1:684265/140‑1 (MQ=255)          gTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGga                                                                                                                            >  2:98815/1‑140 (MQ=255)                                     tctcCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATATTATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTgg                                                                                                 <  2:353977/140‑1 (MQ=255)                                     tctcCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATGAAGGCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTgg                                                                                                 <  2:507100/140‑1 (MQ=255)                                        ccTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGgcag                                                                                              >  1:331253/1‑140 (MQ=255)                                          tttATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGgcagca                                                                                            <  1:935978/140‑1 (MQ=255)                                          tttATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGgcagca                                                                                            <  1:834406/140‑1 (MQ=255)                                           ttATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCAt                                                                                           >  1:526355/1‑140 (MQ=255)                                            tATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATa                                                                                          >  1:798798/1‑140 (MQ=255)                                                  tCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTgg                                                                                                 <  2:792295/127‑1 (MQ=255)                                                  tCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTgg                                                                                                 >  1:792295/1‑127 (MQ=255)                                                  tCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAg                                                                                    >  1:187170/1‑140 (MQ=255)                                                   cATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAgg                                                                                   >  2:58671/1‑140 (MQ=255)                                                   cATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAgg                                                                                   >  1:482732/1‑140 (MQ=255)                                                    aTGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGc                                                                                  <  2:482732/140‑1 (MQ=255)                                                                tACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGt                                                                      >  2:280006/1‑140 (MQ=255)                                                                tACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGt                                                                      <  2:526355/140‑1 (MQ=255)                                                                 aCGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTg                                                                     >  2:191239/1‑140 (MQ=255)                                                                  cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGc                                                                    >  2:490998/1‑140 (MQ=255)                                                                  cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGc                                                                    >  2:529183/1‑140 (MQ=255)                                                                             tctTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGt                                                                      <  1:189461/127‑1 (MQ=255)                                                                             tctTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGt                                                                      >  2:189461/1‑127 (MQ=255)                                                                             tctTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCg                                                         <  2:798798/140‑1 (MQ=255)                                                                                tGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCg                                                      <  1:191239/140‑1 (MQ=255)                                                                                 gCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCg                                                      >  1:843295/1‑139 (MQ=255)                                                                                 gCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCg                                                      <  2:843295/139‑1 (MQ=255)                                                                                 gCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGa                                                     >  2:276707/1‑140 (MQ=255)                                                                                 gCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGa                                                     >  2:1016749/1‑140 (MQ=255)                                                                                     tAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTTAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAAtct                                                 <  2:113640/140‑1 (MQ=255)                                                                                     tAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAAtct                                                 <  1:529183/140‑1 (MQ=255)                                                                                       aaaTGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCa                                               <  1:280006/140‑1 (MQ=255)                                                                                            aataatATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAAtct                                                 >  2:309897/1‑133 (MQ=255)                                                                                            aataatATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAAtct                                                 <  1:309897/133‑1 (MQ=255)                                                                                            aataatATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTAc                                           <  2:300383/139‑1 (MQ=255)                                                                                            aataatATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTAc                                           >  1:300383/1‑139 (MQ=255)                                                                                             ataataTTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAAtct                                                 <  1:950771/132‑1 (MQ=255)                                                                                             ataataTTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAAtct                                                 >  2:950771/1‑132 (MQ=255)                                                                                             ataataTTAAGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTAccc                                         >  2:393604/1‑140 (MQ=255)                                                                                                      aGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAAtct                                                 >  1:718807/1‑123 (MQ=255)                                                                                                      aGCCACTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAAtct                                                 <  2:718807/123‑1 (MQ=255)                                                                                                          aCTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCt                            <  1:490998/140‑1 (MQ=255)                                                                                                           cTTATTCACGAATCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTg                           >  2:712767/1‑140 (MQ=255)                                                                                                                      aTCGAGAATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACt                >  1:393646/1‑140 (MQ=255)                                                                                                                          agaATGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTgggg            >  2:846534/1‑140 (MQ=255)                                                                                                                              tGCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTg        <  2:741824/140‑1 (MQ=255)                                                                                                                               gCTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGa       <  1:98815/140‑1 (MQ=255)                                                                                                                                cTATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGAt      <  2:331253/140‑1 (MQ=255)                                                                                                                                 tATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATc     >  1:766564/1‑140 (MQ=255)                                                                                                                                 tATGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATc     >  1:114259/1‑140 (MQ=255)                                                                                                                                  aTGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGt          >  1:385035/1‑134 (MQ=255)                                                                                                                                  aTGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGt          <  2:385035/134‑1 (MQ=255)                                                                                                                                   tGGATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTg   >  2:861127/1‑140 (MQ=255)                                                                                                                                    ggATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGt  <  2:766564/140‑1 (MQ=255)                                                                                                                                    ggATTTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGt  >  1:860885/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        ATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCTGGAAACAGAACTGGGGGTTGATCTGT  >  NC_000913/1614675‑1614945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑