Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,673,215	A→C	V96G (GTC→GGC)	mdtJ ←	multidrug/spermidine efflux pump membrane subunit MdtJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,673,215	0	A	C	100.0%	132.6 / NA	38	V96G (GTC→GGC)	mdtJ	multidrug/spermidine efflux pump membrane subunit MdtJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/18);  total (20/18)

CGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAG  >  NC_000913/1673080‑1673334                                                                                                                                        |                                                                                                                        cGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAg                                                                                                                     <  2:34838/140‑1 (MQ=255)      gCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGccc                                                                                                                       >  2:281119/1‑133 (MQ=255)          cGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTaa                                                                                                            >  1:831512/1‑140 (MQ=255)               tCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCgg                                                                                                       >  2:509741/1‑140 (MQ=255)                        aTTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTtca                                                                                              >  2:952869/1‑140 (MQ=255)                         ttGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTtcat                                                                                             >  2:840052/1‑140 (MQ=255)                         ttGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTtcat                                                                                             <  1:840423/140‑1 (MQ=255)                         ttGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTtcat                                                                                             <  1:840052/140‑1 (MQ=255)                                     gccagGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACt                                                                                 <  1:952869/140‑1 (MQ=255)                                         ggcggcGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTtcg                                                                             <  1:349426/140‑1 (MQ=255)                                                   aCCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACa                                                                   <  2:672328/140‑1 (MQ=255)                                                   aCCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACa                                                                   <  1:257921/140‑1 (MQ=255)                                                        ttCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACg                                                              <  2:102087/140‑1 (MQ=255)                                                              cTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAAc                                                        <  2:74161/140‑1 (MQ=255)                                                               tGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACa                                                       <  2:346358/140‑1 (MQ=255)                                                               tGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACa                                                       >  1:346358/1‑140 (MQ=255)                                                               tGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACa                                                       >  1:264087/1‑140 (MQ=255)                                                                  gcCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAgg                                                    <  2:264087/140‑1 (MQ=255)                                                                    cATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGta                                                  <  2:360914/140‑1 (MQ=255)                                                                                ccAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAAc                                                        >  1:208043/1‑122 (MQ=255)                                                                                ccAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAAc                                                        <  2:208043/122‑1 (MQ=255)                                                                                 cAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATAc                                     >  1:67787/1‑140 (MQ=255)                                                                                   gTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCg                                   >  1:855203/1‑140 (MQ=255)                                                                                   gTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCg                                   >  2:1017898/1‑140 (MQ=255)                                                                                   gTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCg                                   >  2:796182/1‑140 (MQ=255)                                                                                      cAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATa                                <  1:1017898/140‑1 (MQ=255)                                                                                        ggTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATAcc                              >  2:337711/1‑140 (MQ=255)                                                                                            tACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTcc                          <  1:127274/140‑1 (MQ=255)                                                                                                   tttACGGGTACCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAgcg                   >  1:375995/1‑140 (MQ=255)                                                                                                            aCCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAATTACCGATACCTTCCCGCAGCGCa                 >  2:625972/1‑133 (MQ=255)                                                                                                            aCCTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCa                 <  1:625972/133‑1 (MQ=255)                                                                                                             ccTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCa                 <  2:334774/132‑1 (MQ=255)                                                                                                             ccTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCa                 >  1:334774/1‑132 (MQ=255)                                                                                                             ccTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTAc         >  1:38819/1‑140 (MQ=255)                                                                                                             ccTGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTAc         >  1:147325/1‑140 (MQ=255)                                                                                                               tGATTTTATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGc       >  1:48724/1‑140 (MQ=255)                                                                                                                 aTTTTATCAACACAATCCCGGCGCCCAGGGTGGTTGACCCGGCAACTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCcac                       <  1:729527/122‑1 (MQ=255)                                                                                                                 aTTTTATCAACACAATCCCGGCGCCCAGGGTGGTTGACCCGGCAACTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCcac                       >  2:729527/1‑122 (MQ=255)                                                                                                                    ttATCAACACAATCCCGGCGCCCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAg  >  2:62802/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                        CGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAG  >  NC_000913/1673080‑1673334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑