Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,736,263	G→A	A343V (GCT→GTT)	ydhP ←	putative transporter YdhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,736,263	0	G	A	100.0%	214.7 / NA	61	A343V (GCT→GTT)	ydhP	putative transporter YdhP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/31);  total (30/31)

AGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACAC  >  NC_000913/1736130‑1736396                                                                                                                                      |                                                                                                                                      aGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTc                                                                                                                                 >  1:357120/1‑140 (MQ=255) aGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTc                                                                                                                                 >  1:610559/1‑140 (MQ=255)      cGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAgc                                                                                                                            >  1:43513/1‑140 (MQ=255)            cTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCAttt                                                                                                                      >  2:435853/1‑140 (MQ=255)              gTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTcc                                                                                                                    <  1:918610/140‑1 (MQ=255)               tttCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCa                                                                                                                   <  1:68359/140‑1 (MQ=255)                ttCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCaa                                                                                                                  >  2:794969/1‑140 (MQ=255)                       tgttTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTaaa                                                                                                           <  1:794969/140‑1 (MQ=255)                         ttttCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAgg                                                                                                         >  1:821058/1‑140 (MQ=255)                         ttttCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAgg                                                                                                         >  2:766647/1‑140 (MQ=255)                         ttttCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAgg                                                                                                         <  2:43513/140‑1 (MQ=255)                           ttCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCa                                                                                                       <  1:872882/140‑1 (MQ=255)                           ttCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCAGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCa                                                                                                       <  2:914265/140‑1 (MQ=255)                            tCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCAc                                                                                                      <  2:876669/140‑1 (MQ=255)                            tCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCAc                                                                                                      <  1:766647/140‑1 (MQ=255)                            tCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCAc                                                                                                      <  1:591640/140‑1 (MQ=255)                              tGGCTGACATAAACGCCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTGCCGCTCCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCa                                                                                                    <  1:421020/140‑1 (MQ=255)                               ggCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGACACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCaa                                                                                                   <  2:357120/140‑1 (MQ=255)                                   gACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAgg                                                                                                         >  1:666116/1‑130 (MQ=255)                                   gACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAgg                                                                                                         <  2:666116/130‑1 (MQ=255)                                   gACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCa                                                                                                       >  1:821856/1‑132 (MQ=255)                                   gACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCa                                                                                                       <  2:821856/132‑1 (MQ=255)                                     cATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGa                                                                                             <  2:953426/140‑1 (MQ=255)                                          acacCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACtgat                                                                                        >  2:875568/1‑140 (MQ=255)                                              cAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAATGCACCAATATTGACTGATGAag                                                                                    >  1:527825/1‑140 (MQ=255)                                              cAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACtgat                                                                                        <  2:237251/136‑1 (MQ=255)                                              cAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACtgat                                                                                        >  1:237251/1‑136 (MQ=255)                                              cAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACtga                                                                                         <  2:954511/135‑1 (MQ=255)                                              cAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACtga                                                                                         >  1:954511/1‑135 (MQ=255)                                              cAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAag                                                                                    >  1:199618/1‑140 (MQ=255)                                              cAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAag                                                                                    >  2:136427/1‑140 (MQ=255)                                              cAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAag                                                                                    >  1:531973/1‑140 (MQ=255)                                                gCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAagac                                                                                  >  1:276529/1‑140 (MQ=255)                                                    taatGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGa                                                                                             >  1:838692/1‑125 (MQ=255)                                                    taatGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGa                                                                                             <  2:838692/125‑1 (MQ=255)                                                            gTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGAcc                                                                             >  2:686753/1‑133 (MQ=255)                                                            gTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGAcc                                                                             <  1:686753/133‑1 (MQ=255)                                                              cccGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTc                                                                    >  1:339754/1‑140 (MQ=255)                                                                        tCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGaca                                                          <  2:821058/140‑1 (MQ=255)                                                                          gCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGgcg                                                                        <  1:767860/124‑1 (MQ=255)                                                                          gCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGgcg                                                                        >  2:767860/1‑124 (MQ=255)                                                                             cccATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCAt                                                     >  2:618841/1‑140 (MQ=255)                                                                                 tCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAATGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCAcg                                                 <  2:527825/140‑1 (MQ=255)                                                                                 tCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCAcg                                                 <  1:618841/140‑1 (MQ=255)                                                                                 tCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCAcg                                                 <  2:199618/140‑1 (MQ=255)                                                                                         acaAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTg                                         <  2:531973/140‑1 (MQ=255)                                                                                             aGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAAc                                     >  2:688867/1‑140 (MQ=255)                                                                                                  tATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAAcggcgg                                >  1:975018/1‑140 (MQ=255)                                                                                                        agcccagcGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGAc                          >  2:381319/1‑140 (MQ=255)                                                                                                         gcccagcGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGAcc                         >  1:121922/1‑140 (MQ=255)                                                                                                         gcccagcGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGAcc                         >  2:547817/1‑140 (MQ=255)                                                                                                         gcccagcGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTAAGAcc                         >  1:862100/1‑140 (MQ=255)                                                                                                              gcGGAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCaaa                    >  1:370606/1‑140 (MQ=255)                                                                                                                ggAAATTACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAgg                  <  1:824822/140‑1 (MQ=255)                                                                                                                     ttACCGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTtgct             <  1:875568/140‑1 (MQ=255)                                                                                                                        ccGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTgcg          <  1:333665/140‑1 (MQ=255)                                                                                                                        ccGCACCACCAGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTgcg          <  1:136427/140‑1 (MQ=255)                                                                                                                             ccaccaGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCca     <  2:610559/140‑1 (MQ=255)                                                                                                                             ccaccaGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCca     <  2:339754/140‑1 (MQ=255)                                                                                                                               accaGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCcaca   <  1:547817/140‑1 (MQ=255)                                                                                                                                ccaGCAACTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCacac  >  1:124823/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      AGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGGAAATTACCGCACCACCAGCAGCTGCTCCCAGCGCATTTCCAAGATTAAAGGCACCAATATTGACTGATGAAGACAGACCTGGCGCTTCACTGGCGACACGCATCACGCGCATCTGTAACGGCGGTACGACCGCAAAGGTTGCTGCGCCCCACAC  >  NC_000913/1736130‑1736396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑