Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,753,426	G→A	A135A (GCC→GCT)	ydhV ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,753,426	0	G	A	100.0%	171.7 / NA	49	A135A (GCC→GCT)	ydhV	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/27);  total (22/27)

TTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGA  >  NC_000913/1753289‑1753562                                                                                                                                          |                                                                                                                                         ttACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGc                                                                                                                                        <  2:587310/140‑1 (MQ=255)  tACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCt                                                                                                                                       <  2:246226/140‑1 (MQ=255)  tACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCt                                                                                                                                       <  1:885746/140‑1 (MQ=255)    cGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCttt                                                                                                                                     >  1:186643/1‑140 (MQ=255)      gCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCttttt                                                                                                                                   <  2:112106/140‑1 (MQ=255)              aCATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTa                                                                                                                           <  2:116010/140‑1 (MQ=255)                  gCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACtt                                                                                                                       >  1:143325/1‑140 (MQ=255)                   ccAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACttt                                                                                                                      >  1:901188/1‑140 (MQ=255)                    cAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTg                                                                                                                     >  1:283541/1‑140 (MQ=255)                    cAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTg                                                                                                                     >  1:790923/1‑140 (MQ=255)                            ggAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCttt                                                                                                             >  2:196670/1‑140 (MQ=255)                              aaCAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCtt                                                                                                              <  1:471490/137‑1 (MQ=255)                              aaCAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCtt                                                                                                              >  2:471490/1‑137 (MQ=255)                                 aaGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCt                                                                                                        <  2:283541/140‑1 (MQ=255)                                        tCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCC‑TTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATc                                                                                                         <  2:672822/131‑1 (MQ=255)                                        tCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCC‑TTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATc                                                                                                         >  1:672822/1‑131 (MQ=255)                                              gCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCgg                                                                                           >  1:335794/1‑140 (MQ=255)                                              gCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCgg                                                                                           >  1:790589/1‑140 (MQ=255)                                                  gACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGAt                                                                                       <  1:646403/140‑1 (MQ=255)                                                   aCCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGAtt                                                                                      >  1:191139/1‑140 (MQ=255)                                                        tAGCCCCCACACAGGTTTCCGGACTGCTCAATCGACAAATATCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTTATCTTTAATCTTCAGCCATACCGGTGATTTCGcc                                                                                 <  2:901188/140‑1 (MQ=255)                                                         aGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGcct                                                                                >  2:541786/1‑140 (MQ=255)                                                             gccACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGccttccc                                                                            <  2:335794/140‑1 (MQ=255)                                                             gccACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGccttccc                                                                            <  2:250515/140‑1 (MQ=255)                                                             gccACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGccttccc                                                                            >  2:283201/1‑140 (MQ=255)                                                               cacacaGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGccttccctt                                                                          >  1:872710/1‑140 (MQ=255)                                                                      gTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGataat                                                                   >  2:694918/1‑140 (MQ=255)                                                                         tCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATaataat                                                                >  1:748658/1‑140 (MQ=255)                                                                          ccGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATg                                                               <  2:748658/140‑1 (MQ=255)                                                                          ccGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATg                                                               <  1:633778/140‑1 (MQ=255)                                                                          ccGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATg                                                               <  2:790923/140‑1 (MQ=255)                                                                          ccGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATg                                                               <  2:143325/140‑1 (MQ=255)                                                                             gACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGAcg                                                            <  1:338045/140‑1 (MQ=255)                                                                              aCTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGAcgt                                                           <  2:191139/140‑1 (MQ=255)                                                                                  gTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTa                                                       >  1:137626/1‑140 (MQ=255)                                                                                  gTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTa                                                       >  2:653548/1‑140 (MQ=255)                                                                                  gTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTACGCCTTCCCTTCGATAATAATGACGTCGTa                                                       >  1:568301/1‑140 (MQ=255)                                                                                       tCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAg                                                  <  1:653548/140‑1 (MQ=255)                                                                                       tCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAg                                                  <  1:541786/140‑1 (MQ=255)                                                                                            aaaTTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAAt                                             >  2:576534/1‑140 (MQ=255)                                                                                                    tcCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTACGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTg                                     <  2:568301/140‑1 (MQ=255)                                                                                                      cgtcgtTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTgcg                                   <  2:137626/140‑1 (MQ=255)                                                                                                        tcgtTGCGCGCGTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCt                                 >  2:296002/1‑140 (MQ=255)                                                                                                                  cgTCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGc                       <  1:196670/140‑1 (MQ=255)                                                                                                                    tCCCTTTTCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCa                     <  2:89660/140‑1 (MQ=255)                                                                                                                          ttCCCCATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCata               <  1:14888/140‑1 (MQ=255)                                                                                                                             cccATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATgg            <  1:296002/140‑1 (MQ=255)                                                                                                                               cATAAGAAATCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACTGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGc          >  1:988663/1‑140 (MQ=255)                                                                                                                                       aTCAGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACTGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATcgacga  <  2:988663/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TTACGGCTATTCAACATGCCAGAGAGAGGAACAAGGTTTTCCCCAGCCTGACCAATAGCCGCCACACAGGTTTCCGGACTGGTCAATCGACAAATTTCTTCCGTCGTTGCGCGCGTCCCTTTTCCCCATAAGAAATCGGCTTTTTCCAGGCTAACTTTGTCATCTTTAATCTTCAGCCATACCGGTGATTTCGCCTTCCCTTCGATAATAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGA  >  NC_000913/1753289‑1753562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑