Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	283,866	C→T	G222G (GGC→GGT)	yagF →	CP4‑6 prophage; D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	283,866	0	C	T	100.0%	106.4 / NA	32	G222G (GGC→GGT)	yagF	CP4‑6 prophage; D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/13);  total (19/13)

GGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGT  >  NC_000913/283755‑283996                                                                                                                |                                                                                                                                   gggcggggcgACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGc                                                                                                                 <  1:666434/131‑1 (MQ=255) gggcggggcgACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGc                                                                                                                 >  2:666434/1‑131 (MQ=255) gggcggggcgACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGgcggcg                                                                                                        >  2:293123/1‑140 (MQ=255)  ggcggggcgACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGcggcgg                                                                                                       >  2:753457/1‑140 (MQ=255)                 ccgccgACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCgg                                                                                        >  2:655579/1‑140 (MQ=255)                     cgACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCAcg                                                                                    <  1:753457/140‑1 (MQ=255)                                   gACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACTAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCATTTCCTCGGCACGGCGGGCACCTCGCa                                                                      >  1:546506/1‑140 (MQ=255)                                    aCGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAg                                                                     <  1:293123/140‑1 (MQ=255)                                      gcgGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAggt                                                                   >  2:790491/1‑140 (MQ=255)                                         ggCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCggg                                                                               <  2:845444/125‑1 (MQ=255)                                         ggCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCggg                                                                               >  1:845444/1‑125 (MQ=255)                                                tGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAg                                                         >  2:550696/1‑140 (MQ=255)                                                  cAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGgc                                                       >  1:385020/1‑140 (MQ=255)                                                          cGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCGGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGa                                                          <  2:653014/129‑1 (MQ=255)                                                          cGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCGGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGa                                                          >  1:653014/1‑129 (MQ=255)                                                          cGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTc                                               >  1:233751/1‑140 (MQ=255)                                                               cgcgTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGgcg                                          <  1:229031/140‑1 (MQ=255)                                                               cgcgTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGgcg                                          <  1:790491/140‑1 (MQ=255)                                                               cgcgTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGGCGCGGAGGCGCTGGGTCTGgcg                                          >  1:80331/1‑140 (MQ=255)                                                                       gCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCa                                  <  1:550696/140‑1 (MQ=255)                                                                            ccACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCcg                             >  1:563972/1‑140 (MQ=255)                                                                                      tcCCTGCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGcc                   >  2:830646/1‑140 (MQ=255)                                                                                           gCAGGAGGCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCg              <  2:233751/140‑1 (MQ=255)                                                                                              ggaggCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGc           >  1:945388/1‑140 (MQ=255)                                                                                              ggaggCCGCCGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGGGGGCGCGGAGGCGCTGGGTCTGGCGCGGCCGCACTCCGCGCTGGCGCCGTCCGGGc           >  1:446198/1‑140 (MQ=255)                                                                                                  gccgccGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGGCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGc       >  2:534771/1‑140 (MQ=255)                                                                                                  gccgccGAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCCCCGGTGGTCGCGGCGGCGCTGGGGCTGGCGCTGCCGCACTCCGCGCTGGCGCCGGCCggggcggc       >  2:997374/1‑135 (MQ=255)                                                                                                    cgccgAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCgg     >  1:74068/1‑140 (MQ=255)                                                                                                     gccgAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGgt    <  2:74068/140‑1 (MQ=255)                                                                                                      ccgAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGgtg   <  1:997374/140‑1 (MQ=255)                                                                                                      ccgAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCCGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGgtg   <  2:385020/140‑1 (MQ=255)                                                                                                       cgAACTGGGTTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGgtgt  <  1:830646/140‑1 (MQ=255)                                                                                                                |                                                                                                                                   GGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGT  >  NC_000913/283755‑283996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑