Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,790,861	T→C	I128M (ATA→ATG)	ydiU ←	UPF0061 family protein YdiU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,790,861	0	T	C	100.0%	193.8 / NA	55	I128M (ATA→ATG)	ydiU	UPF0061 family protein YdiU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/21);  total (34/21)

GTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCAC  >  NC_000913/1790728‑1790994                                                                                                                                      |                                                                                                                                      gTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCAtcgtc                                                                                                                                 <  1:361401/140‑1 (MQ=255)   gCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGa                                                                                                                               >  1:830851/1‑140 (MQ=255)       cacGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGt                                                                                                                           >  2:750590/1‑140 (MQ=255)             tCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAAcc                                                                                                                     <  2:213795/140‑1 (MQ=255)                    cgcgCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTc                                                                                                              <  2:492273/140‑1 (MQ=255)                    cgcgCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTc                                                                                                              <  2:536481/140‑1 (MQ=255)                      cgcCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGcc                                                                                                                  <  1:781517/134‑1 (MQ=255)                      cgcCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGcc                                                                                                                  >  2:781517/1‑134 (MQ=255)                      cgcCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCa                                                                                                            >  1:53854/1‑140 (MQ=255)                               ccgccgTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCAtt                                                                                                   <  1:188820/140‑1 (MQ=255)                                 gccgTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTcg                                                                                                 <  2:335473/140‑1 (MQ=255)                                             tACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGt                                                                                     <  2:53854/140‑1 (MQ=255)                                                acTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAg                                                                                  >  1:478572/1‑140 (MQ=255)                                                  tGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGc                                                                                >  1:51363/1‑140 (MQ=255)                                                  tGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGc                                                                                >  2:409648/1‑140 (MQ=255)                                                   ggCGAATCTCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGt                                                                                     >  2:728319/1‑134 (MQ=255)                                                   ggCGAATCTCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGt                                                                                     <  1:728319/134‑1 (MQ=255)                                                    gCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGt                                                                                     <  2:716298/133‑1 (MQ=255)                                                    gCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGt                                                                                     >  1:716298/1‑133 (MQ=255)                                                    gCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCa                                                                              >  2:811134/1‑140 (MQ=255)                                                       aaTCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCa                                                                           >  2:703038/1‑140 (MQ=255)                                                             tGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTc                                                                     <  1:811134/140‑1 (MQ=255)                                                              ggTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCAcc                                                                         >  1:70878/1‑135 (MQ=255)                                                              ggTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCAcc                                                                         <  2:70878/135‑1 (MQ=255)                                                              ggTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGCAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCa                                                                    >  2:588094/1‑140 (MQ=255)                                                                   cGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGc                                                               <  1:750590/140‑1 (MQ=255)                                                                     aTACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCa                                                             <  1:703038/140‑1 (MQ=255)                                                                         ttAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTcc                                                         >  1:415650/1‑140 (MQ=255)                                                                                 cgGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGt                                                 >  2:110954/1‑140 (MQ=255)                                                                                  gggTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGc                                                               <  2:679492/125‑1 (MQ=255)                                                                                  gggTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGc                                                               >  1:679492/1‑125 (MQ=255)                                                                                  gggTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTg                                                >  1:797126/1‑140 (MQ=255)                                                                                   ggTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGc                                               >  2:751815/1‑140 (MQ=255)                                                                                             aTGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCagcaag                                     <  2:478572/140‑1 (MQ=255)                                                                                                   aGATAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAgttg                               >  1:748909/1‑140 (MQ=255)                                                                                                     aTAATGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCTATTGTAGTGCCATCAGCAAGCAgttgtt                             >  1:270327/1‑140 (MQ=255)                                                                                                       aaTGCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCg                           <  1:75269/140‑1 (MQ=255)                                                                                                          gCATCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCg                        >  2:398294/1‑140 (MQ=255)                                                                                                            aTCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAg                      >  1:31074/1‑140 (MQ=255)                                                                                                             tCGCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGt                     >  1:32045/1‑140 (MQ=255)                                                                                                               gCCTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTaa                   >  2:143294/1‑140 (MQ=255)                                                                                                                ccTCACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTaaa                  >  2:355675/1‑140 (MQ=255)                                                                                                                   cACTGGCGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATg               >  2:324078/1‑140 (MQ=255)                                                                                                                         cGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCgcgc         <  2:415650/140‑1 (MQ=255)                                                                                                                         cGAGACTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCgcgc         >  1:627154/1‑140 (MQ=255)                                                                                                                          gagaCTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGcc        <  1:751815/140‑1 (MQ=255)                                                                                                                             aCTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCAt     >  2:374373/1‑140 (MQ=255)                                                                                                                             aCTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCAt     >  1:88467/1‑140 (MQ=255)                                                                                                                              cTTTCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATc    >  1:587404/1‑140 (MQ=255)                                                                                                                               tttCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCa   <  1:355675/140‑1 (MQ=255)                                                                                                                                ttCTCGCATCGTCGGACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCAc  >  1:976845/1‑140 (MQ=255)                                                                                                                                ttCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCAc  >  1:603418/1‑140 (MQ=255)                                                                                                                                ttCTCGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCAc  >  1:480157/1‑140 (MQ=255)                                                                                                                                  ctcGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGccc       <  1:724024/133‑1 (MQ=255)                                                                                                                                  ctcGCATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGccc       >  2:724024/1‑133 (MQ=255)                                                                                                                                      |                                                                                                                                      GTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCGTTTCCCGATACACTGGCGAATCGCTGGTGACGATACTTAACGCGCGGGTCGTCGGAATGCCCAGATAATGCATCGCCTCACTGGCGAGACTTTCTCGTATCGTCGAACGTAAAACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCAC  >  NC_000913/1790728‑1790994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑