Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,924,089	A→G	V168A (GTG→GCG)	yebZ ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,924,089	0	A	G	100.0%	130.1 / NA	38	V168A (GTG→GCG)	yebZ	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/19);  total (19/19)

TCAACAATGCATTAAGCACGCCGCTTGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAG  >  NC_000913/1923958‑1924224                                                                                                                                    |                                                                                                                                        tCAACAATGCATTAAGCACGCCGCTTGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGt                                                                                                                                 >  1:290503/1‑140 (MQ=255)  caacaaTGCATTAAGCACGCCGCTTGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTa                                                                                                                                <  2:690858/140‑1 (MQ=255)           aTTAAGCACGCCGCTTGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCaa                                                                                                                       >  1:569778/1‑140 (MQ=255)                    gccgcTTGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCTGGCAGTAATCCCCCAAACCAcgccg                                                                                                              >  2:316860/1‑140 (MQ=255)                    gccgcTTGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCAcgccg                                                                                                              >  2:306296/1‑140 (MQ=255)                    gccgcTTGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCAcgccg                                                                                                              >  1:456482/1‑140 (MQ=255)                       gcTTGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCAcgccgccg                                                                                                           >  1:371848/1‑140 (MQ=255)                       gcTTGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGACGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCAcgccgccg                                                                                                           >  2:985418/1‑140 (MQ=255)                         ttGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGca                                                                                                         >  2:858451/1‑140 (MQ=255)                         ttGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGca                                                                                                         <  2:290503/140‑1 (MQ=255)                         ttGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGca                                                                                                         <  1:858451/140‑1 (MQ=255)                                       aTCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGTATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAAt                                                                                           >  1:919896/1‑140 (MQ=255)                                            cGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGt                                                                                      >  2:1000202/1‑140 (MQ=255)                                               aaaaTGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGAt                                                                                   <  2:889521/140‑1 (MQ=255)                                                 aaTGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTa                                                                                 >  2:54404/1‑140 (MQ=255)                                                      ccGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTg                                                                            >  1:298366/1‑140 (MQ=255)                                                             aGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGAtt                                                                     <  2:569778/140‑1 (MQ=255)                                                                aaaaaCGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTc                                                                  >  2:69202/1‑140 (MQ=255)                                                                     cGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGt                                                             <  1:775203/140‑1 (MQ=255)                                                                      gCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGtt                                                            >  1:517211/1‑140 (MQ=255)                                                                       cATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTa                                                           <  2:456482/140‑1 (MQ=255)                                                                                 tGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCttcatt                                                 <  1:1000202/140‑1 (MQ=255)                                                                                  gAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCttcattc                                                >  1:770413/1‑140 (MQ=255)                                                                                    aTAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAg                                              <  2:517211/140‑1 (MQ=255)                                                                                            gtgtCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCAt                                      >  2:550356/1‑140 (MQ=255)                                                                                               tCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGcc                                   >  1:295961/1‑140 (MQ=255)                                                                                               tCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGcc                                   <  2:371848/140‑1 (MQ=255)                                                                                                 gCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGcccc                                 >  1:102590/1‑140 (MQ=255)                                                                                                 gCCAACGGCCTTTGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGcccc                                 <  1:68096/140‑1 (MQ=255)                                                                                                            ttGATGAGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCGCTccagccag                      <  1:819784/140‑1 (MQ=255)                                                                                                             tgatgaGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGc                         >  2:62093/1‑136 (MQ=255)                                                                                                             tgatgaGTTGCATACACCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGc                         <  1:62093/136‑1 (MQ=255)                                                                                                                     tGCATACCCCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATaaa             <  1:579814/140‑1 (MQ=255)                                                                                                                          acacCAGAGCGCTGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTgcg        <  1:316860/140‑1 (MQ=255)                                                                                                                          acacCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTgcg        <  1:306296/140‑1 (MQ=255)                                                                                                                          acacCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTgcg        <  1:550356/140‑1 (MQ=255)                                                                                                                          acacCAGAGCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTgcg        <  2:919896/140‑1 (MQ=255)                                                                                                                                gagCGCCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAg  <  2:102590/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        TCAACAATGCATTAAGCACGCCGCTTGCCAGTACGCCGATCACCGCAAAATGCCCGCACCAGGAAAAACGCATCAGCGCCTGAATAGCCTGGTGTCGCCAACGGCCTTTGATGAGTTGCATACACCAGAGCACCGGCAGTAATCCCCCAAACCACGCCGCCGCACAAATCAGGTGAATCGCGTGATTAGTCTGATGGATTTTCGCTGTTACCCCTTCATTCAGCGTCGCATGCCCCACTCCCGCCAGCAGAATAAATTGCGCGGTAG  >  NC_000913/1923958‑1924224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑