Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	373,802	T→C	T294T (ACT→ACC)	mhpF →	acetaldehyde dehydrogenase (acylating)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	373,802	0	T	C	100.0%	154.3 / NA	45	T294T (ACT→ACC)	mhpF	acetaldehyde dehydrogenase (acylating)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/21);  total (24/21)

ATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTA  >  NC_000913/373671‑373938                                                                                                                                    |                                                                                                                                         aTCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTATCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTgc                                                                                                                                  >  2:418056/1‑140 (MQ=255)  tCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTgcg                                                                                                                                 >  2:785488/1‑140 (MQ=255)  tCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTgcg                                                                                                                                 <  2:152895/140‑1 (MQ=255)                    tCAATTTACCGGGCGTGGGGCAATTCTCCGTACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGaaaaa                                                                                                               >  1:997741/1‑140 (MQ=255)                    tCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGaaaaa                                                                                                               >  2:774134/1‑140 (MQ=255)                    tCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGaaaaa                                                                                                               >  1:804913/1‑140 (MQ=255)                        tttACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATgg                                                                                                           >  2:157298/1‑140 (MQ=255)                         ttACCGGGCGTGGGGCAATTCTCCGTACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGc                                                                                                          <  2:997741/140‑1 (MQ=255)                         ttACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGc                                                                                                          <  1:157298/140‑1 (MQ=255)                         ttACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGc                                                                                                          <  1:640797/140‑1 (MQ=255)                         ttACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGc                                                                                                          <  1:733483/140‑1 (MQ=255)                                gCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCa                                                                                                   <  1:785488/140‑1 (MQ=255)                                  gTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACt                                                                                                 >  2:3974/1‑140 (MQ=255)                                    ggggCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTgg                                                                                               >  2:312134/1‑140 (MQ=255)                                        cAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGgcgcg                                                                                           <  1:3974/140‑1 (MQ=255)                                           ttCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCaa                                                                                        <  1:874792/140‑1 (MQ=255)                                           ttCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCaa                                                                                        >  2:27524/1‑140 (MQ=255)                                            tctcCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTg                                                                                                >  1:629742/1‑131 (MQ=255)                                            tctcCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTACCTGCATGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTg                                                                                                <  2:629742/131‑1 (MQ=255)                                              tcCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGc                                                                                     >  1:564325/1‑140 (MQ=255)                                                cGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAg                                                                                   >  2:545491/1‑140 (MQ=255)                                                     tGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGaa                                                                              <  2:564325/140‑1 (MQ=255)                                                               ggTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGaa                                                                    >  2:87765/1‑140 (MQ=255)                                                                 tCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACg                                                                  >  1:495516/1‑140 (MQ=255)                                                                      ctggAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTaaa                                                             >  1:29844/1‑140 (MQ=255)                                                                       tggAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTaaaa                                                            <  2:29844/140‑1 (MQ=255)                                                                           aGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACt                                                        >  2:722271/1‑140 (MQ=255)                                                                                   gcgcagcgcaTTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATAtct                                                <  1:722271/140‑1 (MQ=255)                                                                                   gcgcagcgcaTTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATAtct                                                >  2:701992/1‑140 (MQ=255)                                                                                    cgcagcgcaTTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATAtctc                                               >  1:40003/1‑140 (MQ=255)                                                                                    cgcagcgcaTTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATAtctc                                               <  1:87765/140‑1 (MQ=255)                                                                                    cgcagcgcaTTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATAtctc                                               <  2:819610/140‑1 (MQ=255)                                                                                      cagcgcaTTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCgg                                             <  1:27524/140‑1 (MQ=255)                                                                                        gcgcaTTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGAc                                           <  2:723323/140‑1 (MQ=255)                                                                                         cgcaTTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACg                                          >  1:204820/1‑140 (MQ=255)                                                                                              tATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTcaca                                     >  2:664710/1‑140 (MQ=255)                                                                                                   gcctgcctATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCg                                <  1:312134/140‑1 (MQ=255)                                                                                                        cctATGCGGGCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACg                           <  1:664710/140‑1 (MQ=255)                                                                                                               gggCAACCTCGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCa                    <  2:40003/140‑1 (MQ=255)                                                                                                                        cGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCa               <  1:644773/136‑1 (MQ=255)                                                                                                                        cGACATTATGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCa               >  2:644773/1‑136 (MQ=255)                                                                                                                               aTGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAg    >  2:182608/1‑140 (MQ=255)                                                                                                                                tGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGt   >  2:837225/1‑140 (MQ=255)                                                                                                                                tGACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGt   >  1:434767/1‑140 (MQ=255)                                                                                                                                 gACCTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTa  <  1:47489/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         ATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAACGGTAAAAAACTTTATATCTCGGACGTCACATTGCGTGACGGTATGCACGCCATTCGTCATCAGTA  >  NC_000913/373671‑373938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑