Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,942,675	T→C	V5A (GTT→GCT)	znuC →	Zn(2(+)) ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,942,675	0	T	C	100.0%	200.5 / NA	57	V5A (GTT→GCT)	znuC	Zn(2(+)) ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/24);  total (33/24)

AGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGTAC  >  NC_000913/1942539‑1942803                                                                                                                                         |                                                                                                                                 agCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTc                                                                                                                               >  1:547964/1‑140 (MQ=255)   cggcggATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTccc                                                                                                                             >  1:552056/1‑140 (MQ=255)      cggATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTgg                                                                                                                          >  2:451084/1‑140 (MQ=255)                   gAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGt                                                                                                             <  2:547964/140‑1 (MQ=255)                       agaagCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCt                                                                                                         <  1:237829/140‑1 (MQ=255)                                      tGTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAAGTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAAcgccgc                                                                                          <  2:31080/140‑1 (MQ=255)                                           cATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCct                                                                                     <  1:483677/140‑1 (MQ=255)                                               aTGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCctctct                                                                                 >  2:342688/1‑140 (MQ=255)                                                tGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTg                                                                                >  1:383630/1‑140 (MQ=255)                                                tGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTg                                                                                >  1:40084/1‑140 (MQ=255)                                                tGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTg                                                                                >  1:558375/1‑140 (MQ=255)                                                     ccAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGAtgtg                                                                           >  1:407315/1‑140 (MQ=255)                                                     ccAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGAtgtg                                                                           >  2:739332/1‑140 (MQ=255)                                                                    aaTATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTaaa                                                            >  1:545575/1‑140 (MQ=255)                                                                      tatGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAAcc                                                          <  2:558375/140‑1 (MQ=255)                                                                          agaAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGa                                                      >  1:361050/1‑140 (MQ=255)                                                                           gaAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGaa                                                     <  2:375066/140‑1 (MQ=255)                                                                              gtgtGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGaaaaa                                                  >  1:306979/1‑140 (MQ=255)                                                                                 tgATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAAc                                                           <  2:715700/128‑1 (MQ=255)                                                                                 tgATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAAc                                                           >  1:715700/1‑128 (MQ=255)                                                                                 tgATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAAttt                                               >  2:98641/1‑140 (MQ=255)                                                                                  gATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAAtttt                                              <  1:98641/140‑1 (MQ=255)                                                                                         taACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTa                                       >  1:259969/1‑140 (MQ=255)                                                                                         taACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTa                                       >  2:359482/1‑140 (MQ=255)                                                                                               ttCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTggg                                 <  2:40084/140‑1 (MQ=255)                                                                                               ttCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTggg                                 >  2:61630/1‑140 (MQ=255)                                                                                                 cATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGcc                               >  1:564245/1‑140 (MQ=255)                                                                                                  aTGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCa                              <  1:61630/140‑1 (MQ=255)                                                                                                         ctGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTAc                                      >  2:451638/1‑125 (MQ=255)                                                                                                         ctGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTAc                                      <  1:451638/125‑1 (MQ=255)                                                                                                         ctGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGgcg                       >  1:84872/1‑140 (MQ=255)                                                                                                         ctGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGgcg                       >  2:613958/1‑140 (MQ=255)                                                                                                         ctGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGgcg                       >  1:396348/1‑140 (MQ=255)                                                                                                          tGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGgcgc                      >  2:284719/1‑140 (MQ=255)                                                                                                          tGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGgcgc                      >  2:150885/1‑140 (MQ=255)                                                                                                          tGCAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGgcgc                      >  2:188805/1‑140 (MQ=255)                                                                                                            cAAGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAg                    >  1:458492/1‑140 (MQ=255)                                                                                                              aGACTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGt                  <  2:383630/140‑1 (MQ=255)                                                                                                                 cTAAAATTAACATGACAAGTCTGGCTTTCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGTCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTTAg               >  2:586970/1‑140 (MQ=255)                                                                                                                 cTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTggg                                 >  2:315880/1‑122 (MQ=255)                                                                                                                 cTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTggg                                 <  1:315880/122‑1 (MQ=255)                                                                                                                 cTAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAg               >  1:139664/1‑140 (MQ=255)                                                                                                                  tAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGt              <  1:150885/140‑1 (MQ=255)                                                                                                                  tAAAATTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGt              <  1:613958/140‑1 (MQ=255)                                                                                                                   aaaaTTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCaaa                            >  1:568808/1‑125 (MQ=255)                                                                                                                   aaaaTTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCaaa                            <  2:568808/125‑1 (MQ=255)                                                                                                                   aaaaTTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTc             <  2:552056/140‑1 (MQ=255)                                                                                                                    aaaTTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCg            >  1:796113/1‑140 (MQ=255)                                                                                                                    aaaTTAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCg            >  1:916523/1‑140 (MQ=255)                                                                                                                       ttAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGaca         <  2:545575/140‑1 (MQ=255)                                                                                                                       ttAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGaca         <  2:396348/140‑1 (MQ=255)                                                                                                                       ttAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGaca         <  1:359482/140‑1 (MQ=255)                                                                                                                       ttAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGaca         <  1:284719/140‑1 (MQ=255)                                                                                                                       ttAACATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGaca         <  2:916523/140‑1 (MQ=255)                                                                                                                         aaCATGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACt       >  2:737031/1‑140 (MQ=255)                                                                                                                            aTGACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGt    <  1:739332/140‑1 (MQ=255)                                                                                                                              gACAAGTCTGGCTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGTAc  <  1:342688/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 AGCGGCGGATAATGCTGCGAAAAGAAGCGTTTTTTTATGTAACATAATGCGACCAATAATCGTAATGAATATGAGAAGTGTGATATTATAACATTTCATGACTACTGCAAGACTAAAATTAACATGACAAGTCTGGTTTCCCTGGAAAATGTCTCGGTTTCTTTTGGCCAACGCCGCGTCCTCTCTGATGTGTCGCTGGAACTTAAACCTGGAAAAATTTTGACTTTACTTGGGCCAAATGGCGCAGGTAAGTCGACACTGGTAC  >  NC_000913/1942539‑1942803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑