Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,114,034	G→A	T98T (ACC→ACT)	galF ←	UTP:glucose‑1‑phosphate uridylyltransferase, low activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,114,034	0	G	A	100.0%	157.2 / NA	45	T98T (ACC→ACT)	galF	UTP:glucose‑1‑phosphate uridylyltransferase, low activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/18);  total (27/18)

AGCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTGGACGCGTGAGTTACCAG  >  NC_000913/2113900‑2114168                                                                                                                                       |                                                                                                                                       aGCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCAc                                                                                                                                   >  2:726618/1‑140 (MQ=255)  gCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACg                                                                                                                                  >  1:105383/1‑140 (MQ=255)  gCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACg                                                                                                                                  >  2:123693/1‑140 (MQ=255)   cGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCGCACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGc                                                                                                                                 >  2:42377/1‑140 (MQ=255)   cGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAAGGGCAGGTCGCGCGCACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGc                                                                                                                                 >  2:42376/1‑140 (MQ=255)    gggTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGcc                                                                                                                                >  1:399114/1‑140 (MQ=255)        cGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCcggc                                                                                                                            >  2:1004413/1‑140 (MQ=255)             cTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAAACTAAAGGGTCGCCCTGACGCACGTTCATAATAGTCACGCCCCGCGGGCa                                                                                                                       >  2:775704/1‑140 (MQ=255)              tGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGTGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAg                                                                                                                      <  2:810317/140‑1 (MQ=255)                               aCGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGc                                                                                                     <  2:252121/140‑1 (MQ=255)                                cGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGcc                                                                                                    >  2:706978/1‑140 (MQ=255)                                 gTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCca                                                                                                   <  2:681720/140‑1 (MQ=255)                                  tCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCcag                                                                                                  >  2:753700/1‑140 (MQ=255)                                     ggCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCACAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCcagcag                                                                                               >  1:702455/1‑140 (MQ=255)                                        aGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTg                                                                                            <  2:105383/140‑1 (MQ=255)                                            ccACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGc                                                                                        >  2:139654/1‑140 (MQ=255)                                                     aTGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACgcgc                                                                               <  1:464769/140‑1 (MQ=255)                                                            gTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACgcgc                                                                               <  2:323309/133‑1 (MQ=255)                                                            gTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACgcgc                                                                               >  1:323309/1‑133 (MQ=255)                                                               aCCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGa                                                                     <  2:761844/140‑1 (MQ=255)                                                                ccAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAg                                                                    <  1:1004413/140‑1 (MQ=255)                                                                ccAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAg                                                                    <  1:123693/140‑1 (MQ=255)                                                                       cAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCt                                                             >  1:850648/1‑140 (MQ=255)                                                                         ggTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTaa                                                           <  1:706978/140‑1 (MQ=255)                                                                           tCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGGCAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTc                                                              >  1:895440/1‑135 (MQ=255)                                                                           tCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGGCAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTc                                                              <  2:895440/135‑1 (MQ=255)                                                                            cgcgCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTc                                                        <  2:850648/140‑1 (MQ=255)                                                                              cgcACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTc                                                              <  2:214793/132‑1 (MQ=255)                                                                              cgcACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTc                                                              >  1:214793/1‑132 (MQ=255)                                                                              cgcACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCAt                                                      >  2:686409/1‑140 (MQ=255)                                                                                            aGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAg                                        <  1:432672/140‑1 (MQ=255)                                                                                             gTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGt                                       >  2:19021/1‑140 (MQ=255)                                                                                               ggCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTgg                                     >  2:810133/1‑140 (MQ=255)                                                                                                 cctaaacctaaaGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGtt                                   >  1:870531/1‑140 (MQ=255)                                                                                                     aacctaaaGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCg                               >  1:724008/1‑140 (MQ=255)                                                                                                     aacctaaaGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCg                               >  1:206814/1‑140 (MQ=255)                                                                                                        ctaaaGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGAcc                            <  2:94488/140‑1 (MQ=255)                                                                                                        ctaaaGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGAcc                            <  1:42377/140‑1 (MQ=255)                                                                                                          aaaGGTTCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACcgc                          >  2:685671/1‑140 (MQ=255)                                                                                                               ttCGCCCTGACGCACGTTCATAATAGTCACGCCCGGTGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCt                     >  2:640043/1‑140 (MQ=255)                                                                                                               ttCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGAcc                            >  1:893912/1‑133 (MQ=255)                                                                                                               ttCGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGAcc                            <  2:893912/133‑1 (MQ=255)                                                                                                                 cGCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTg                   >  1:587700/1‑140 (MQ=255)                                                                                                                  gCCCTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTgg                  >  1:1019548/1‑140 (MQ=255)                                                                                                                     cTGACGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTGGAcg               <  1:686409/140‑1 (MQ=255)                                                                                                                        aCGCACGTTCATAATAGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTGGACGCGt            >  2:725820/1‑140 (MQ=255)                                                                                                                                  ataataGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTGGACGCGTGAGTTACCag  <  2:587700/140‑1 (MQ=255)                                                                                                                                  ataataGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTGGACGCGTGAGTTACCag  >  2:771857/1‑140 (MQ=255)                                                                                                                                  ataataGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTGGACGCGTGAGTTACCag  >  2:80410/1‑140 (MQ=255)                                                                                                                                  ataataGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTGGACGCGTGAGTTACCag  <  1:775704/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       AGCGGGTCGGCGCTGGCATCGTCGATCACAACGTCTGGCAGTACCACGACAAATGGGTTGTCACCAATGGCAGGTCGCGCACACAAAATGGAGTGGCCTAAACCTAAAGGTTCGCCCTGACGCACGTTCATAATGGTCACGCCCGGCGGACAGATGGACTGTACTTCCGCCAGCAGTTGACGCTTCACGCGCTGCTCAAGGAGTGATTCTAACTCATAAGAGGTGTCGAAGTGGTTTTCGACCGCGTTCTTGGACGCGTGAGTTACCAG  >  NC_000913/2113900‑2114168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑