BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,134,956	T→C	T233A (ACT→GCT)	wzc ←	protein‑tyrosine kinase Wzc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,134,956	0	T	C	100.0%	117.8 / NA	34	T233A (ACT→GCT)	wzc	protein‑tyrosine kinase Wzc
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/16); total (18/16)

TTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTGGTGACGGTAAACTCACTGCCCGGGCTGGCGTGAATGGCTTCAACC  >  NC_000913/2134819‑2135089

ttCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCa                                                                                                                                     >  1:858462/1‑140 (MQ=255)

    ggTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCt                                                                                                                                 <  2:367939/140‑1 (MQ=255)

        aCTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCaaa                                                                                                                             >  2:47689/1‑140 (MQ=255)

          tGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAAc                                                                                                                           >  2:850100/1‑140 (MQ=255)

                                                    ttGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTg                                                                                 <  2:848761/140‑1 (MQ=255)

                                                      gcgcTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCa                                                                               <  2:858462/140‑1 (MQ=255)

                                                      gcgcTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCa                                                                               <  1:663813/140‑1 (MQ=255)

                                                            aaTATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAgttggt                                                                         <  1:669915/140‑1 (MQ=255)

                                                              tatTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAgttggttg                                                                       >  1:877316/1‑140 (MQ=255)

                                                              tatTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAgttggttg                                                                       >  2:336687/1‑140 (MQ=255)

                                                                      tCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCATGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATccc                                                               >  1:482474/1‑140 (MQ=255)

                                                                      tCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGATATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATcc                                                                <  1:153283/139‑1 (MQ=255)

                                                                      tCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGATATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATcc                                                                >  2:153283/1‑139 (MQ=255)

                                                                        cTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATccc                                                               <  2:451469/138‑1 (MQ=255)

                                                                        cTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATccc                                                               >  1:451469/1‑138 (MQ=255)

                                                                        cTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCa                                                             <  1:336687/140‑1 (MQ=255)

                                                                             aGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCATGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTg                                                        <  2:482474/140‑1 (MQ=255)

                                                                               ttACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGa                                                      <  1:47689/140‑1 (MQ=255)

                                                                                  cGGGCGATGCTGTTAAGAATGTTGCGGATCTGTTCACGATCTTCGCCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGt                                                         <  2:603359/134‑1 (MQ=255)

                                                                                  cGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGt                                                         >  1:603359/1‑134 (MQ=255)

                                                                                   gggCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTAt                                                  <  1:142620/140‑1 (MQ=255)

                                                                                       gATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTgg                                              <  1:354973/140‑1 (MQ=255)

                                                                                       gATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTgg                                              >  2:354973/1‑140 (MQ=255)

                                                                                         tGCTGTTATGATTGTCGCGGATCTGTTCACGATCTTCACCAGTATAAGCCCGGCTCAAAACGCCAGCGTCTTCGCATTTCTCCGCTGCCGTCAGGCTGTCTTGCAGTTGGTTGATCGTACCCAGCCTGGAGAAtttaatg                                            <  2:239337/140‑5 (MQ=255)

                                                                                         tGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTGGTg                                            >  2:207297/1‑140 (MQ=255)

                                                                                         tGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTCGATCATCCCCAGCGTGGAGTa                                                   <  1:808724/133‑1 (MQ=255)

                                                                                         tGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTCGATCATCCCCAGCGTGGAGTa                                                   >  2:808724/1‑133 (MQ=255)

                                                                                               tAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTGGTGACGGTa                                      <  1:686505/140‑1 (MQ=255)

                                                                                                     tGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTGGTGACGGTAAACTCa                                >  2:272553/1‑140 (MQ=255)

                                                                                                        cgcgGATCTGTTCACGATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTGGTGACGGTAAACTCACTg                             >  1:758276/1‑140 (MQ=255)

                                                                                                                       gATCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTGGTGACGGTAAACTCACTGCCCGGGCTGGCGTGa              >  1:497819/1‑140 (MQ=255)

                                                                                                                         tCTTCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTGGTGACGGTAAACTCACTGCCCGGGCTGGCGTGAAt            >  1:60013/1‑140 (MQ=255)

                                                                                                                           ttCACCGGTATAAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTGGTGACGGTAAACTCACTGCCCGGGCTGGCGTGAATgg          <  1:850100/140‑1 (MQ=255)

                                                                                                                                   tataAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTGGTGACGGTAAACTCACTGCCCGGGCTGGCGTGAATGGCTTCa     >  1:437887/1‑137 (MQ=255)

                                                                                                                                   tataAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTGGTGACGGTAAACTCACTGCCCGGGCTGGCGTGAATGGCTTCa     <  2:437887/137‑1 (MQ=255)

                                                                                                                                   tataAGCCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTGGTGACGGTAAACTCACTGCCCGGGCTGGCGTGAATGGCTTCAAcc  >  1:92118/1‑140 (MQ=255)

TTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTCGACGCTTCCGCCGATTTGCGCTCAATATTTTGTTCCTGATAGTTACGGGCGATGCTGTTAAGAATGTCGCGGATCTGTTCACGATCTTCACCGGTATAAGTCAGGCTCAAAACGCCAGCGTCTTTGCCGTTCTCCGTTACCGTCAGGCTGTTTTGCAGTTGGTTGATCATCCCCAGCGTGGAGTATTTGGTGACGGTAAACTCACTGCCCGGGCTGGCGTGAATGGCTTCAACC  >  NC_000913/2134819‑2135089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`