Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,226,089	A→G	I97V (ATT→GTT)	yohD →	DedA family protein YohD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,226,089	0	A	G	100.0%	214.1 / NA	60	I97V (ATT→GTT)	yohD	DedA family protein YohD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/31);  total (29/31)

CATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGTTGATTTTTACCACTATT  >  NC_000913/2225956‑2226223                                                                                                                                      |                                                                                                                                       cATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTa                                                                                                                                  <  2:578676/140‑1 (MQ=255)  aTGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTAc                                                                                                                                 <  1:181145/140‑1 (MQ=255)   tGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTAcg                                                                                                                                <  2:650945/140‑1 (MQ=255)   tGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTAcg                                                                                                                                <  2:761902/140‑1 (MQ=255)       tGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCt                                                                                                                            <  1:58262/140‑1 (MQ=255)         gCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCttt                                                                                                                          <  1:585425/140‑1 (MQ=255)             ccAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTtatgt                                                                                                                      >  2:580260/1‑140 (MQ=255)              cAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTtatgta                                                                                                                     <  2:449885/140‑1 (MQ=255)                   gCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCt                                                                                                                <  2:678175/140‑1 (MQ=255)                   gCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCt                                                                                                                <  2:932039/140‑1 (MQ=255)                       tATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCg                                                                                                            >  2:372952/1‑140 (MQ=255)                        aTCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCgg                                                                                                           <  1:883322/140‑1 (MQ=255)                         tCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCt                                                                                                                            >  2:318146/1‑122 (MQ=255)                         tCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCt                                                                                                                            <  1:318146/122‑1 (MQ=255)                            gtgCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGa                                                                                                       >  1:782184/1‑140 (MQ=255)                            gtgCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGa                                                                                                       >  1:784981/1‑140 (MQ=255)                             tgCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTtatgta                                                                                                                     >  2:19339/1‑125 (MQ=255)                             tgCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTtatgta                                                                                                                     <  1:19339/125‑1 (MQ=255)                             tgCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGAt                                                                                                      <  1:372952/140‑1 (MQ=255)                             tgCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGAt                                                                                                      <  2:782184/140‑1 (MQ=255)                              gcgggcggCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGAtt                                                                                                     >  1:895830/1‑140 (MQ=255)                               cgggcggCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTg                                                                                                    >  2:45824/1‑140 (MQ=255)                               cgggcggCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTg                                                                                                    >  2:785509/1‑140 (MQ=255)                                    ggcggTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCg                                                                                               <  2:499745/140‑1 (MQ=255)                                            ggcggcAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGAt                                                                                       <  1:192049/140‑1 (MQ=255)                                                   aGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTgcc                                                                                >  1:948366/1‑140 (MQ=255)                                                             ccGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGt                                                                                   >  1:473423/1‑127 (MQ=255)                                                             ccGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGt                                                                                   <  2:473423/127‑1 (MQ=255)                                                             ccGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTgc                                                                      <  1:785509/140‑1 (MQ=255)                                                                tttCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTgccgc                                                                   >  2:591383/1‑140 (MQ=255)                                                                            tCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTc                                                       <  2:906762/140‑1 (MQ=255)                                                                            tCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTc                                                       <  2:382582/140‑1 (MQ=255)                                                                              aGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTg                                                     <  2:895830/140‑1 (MQ=255)                                                                                gATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGcc                                                   <  1:580260/140‑1 (MQ=255)                                                                                gATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGcc                                                   <  1:591383/140‑1 (MQ=255)                                                                                  tAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGc                                                 >  2:118202/1‑140 (MQ=255)                                                                                       ttGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAat                                            <  2:784981/140‑1 (MQ=255)                                                                                          aGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATAtt                                         >  2:115695/1‑140 (MQ=255)                                                                                          aGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATAtt                                         >  1:334528/1‑140 (MQ=255)                                                                                          aGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATAtt                                         <  2:334528/140‑1 (MQ=255)                                                                                          aGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATAtt                                         >  1:790740/1‑140 (MQ=255)                                                                                          aGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATAtt                                         >  2:43246/1‑140 (MQ=255)                                                                                           gCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATtc                                        >  1:894082/1‑140 (MQ=255)                                                                                               gcgcAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATtct                                       >  2:501113/1‑137 (MQ=255)                                                                                               gcgcAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATtct                                       <  1:501113/137‑1 (MQ=255)                                                                                                          tATCCAACGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCt                         >  2:679519/1‑140 (MQ=255)                                                                                                                 cGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGtt                  >  1:472711/1‑140 (MQ=255)                                                                                                                 cGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGtt                  <  1:45824/140‑1 (MQ=255)                                                                                                                 cGCCATCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGtt                  >  1:858959/1‑140 (MQ=255)                                                                                                                      tCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCTGCGCATTTGCCTGGGCGTTGAtt              >  1:881298/1‑139 (MQ=255)                                                                                                                      tCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCTGCGCATTTGCCTGGGCGTTGAtt              <  2:881298/139‑1 (MQ=255)                                                                                                                      tCCGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGTTGAttt             >  2:633972/1‑140 (MQ=255)                                                                                                                       ccGTATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGTTGAtttt            <  2:472711/140‑1 (MQ=255)                                                                                                                          tATCTGTTTGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGTTGATTTTTAc         >  2:314321/1‑140 (MQ=255)                                                                                                                                tttGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGTTGATTTTTACCACTAt   >  2:843254/1‑140 (MQ=255)                                                                                                                                tttGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGTTGATTTTTACCACTAt   >  2:894329/1‑140 (MQ=255)                                                                                                                                tttGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGTTGATTTTTACCACTAt   >  1:928556/1‑140 (MQ=255)                                                                                                                                 ttGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGTTGATTTTTACCACTAtt  <  2:948366/140‑1 (MQ=255)                                                                                                                                  tGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGTTGATTTTTACCACt     >  1:898195/1‑136 (MQ=255)                                                                                                                                  tGTCGTTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGTTGATTTTTACCACt     <  2:898195/136‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       CATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGTTGATTTTTACCACTATT  >  NC_000913/2225956‑2226223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑