Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,297,856	T→C	E135G (GAG→GGG)	napC ←	periplasmic nitrate reductase cytochrome c protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,297,856	0	T	C	100.0%	174.8 / NA	49	E135G (GAG→GGG)	napC	periplasmic nitrate reductase cytochrome c protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/23);  total (26/23)

ATACAGGTTTGCCCATCTTTCACCGCCTGGTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACTCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCTTTGAGCTTGCGTATCATCTTCGGCACAAACTCGTGCGGA  >  NC_000913/2297721‑2297984                                                                                                                                        |                                                                                                                                 atACAGGTTTGCCCATCTTTCACCGCCTGGTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCAtt                                                                                                                              <  1:996351/140‑1 (MQ=255)   aCAGGTTTGCCCATCTTTCACCGCCTGGTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCt                                                                                                                            <  1:801781/140‑1 (MQ=255)       gTTTGCCCATCTTTCACCGCCTGGTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGc                                                                                                                        <  2:323299/140‑1 (MQ=255)        tttGCCCATCTTTCACCGCCTGGTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGcc                                                                                                                       <  2:967249/140‑1 (MQ=255)          tGCCCATCTTTCACCGCCTGGTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCAt                                                                                                                     >  2:385508/1‑140 (MQ=255)          tGCCCATCTTTCACCGCCTGGTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCAt                                                                                                                     >  2:808119/1‑140 (MQ=255)          tGCCCATCTTTCACCGCCTGGTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCAt                                                                                                                     >  1:108902/1‑140 (MQ=255)            cccATCTTTCACCGCCTGGTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGcc                                                                                                                       <  2:683521/136‑1 (MQ=255)            cccATCTTTCACCGCCTGGTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGcc                                                                                                                       >  1:683521/1‑136 (MQ=255)              cATCTTTCACCGCCTGGTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTc                                                                                                                 <  1:171640/140‑1 (MQ=255)                      aCCGCCTGGTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATg                                                                                                         >  2:1005448/1‑140 (MQ=255)                          ccTGGTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCt                                                                                                     >  2:36967/1‑140 (MQ=255)                            tGGTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTc                                                                                                   >  2:161927/1‑140 (MQ=255)                             ggTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCa                                                                                                  <  1:814612/140‑1 (MQ=255)                              gTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCaa                                                                                                 >  2:649552/1‑140 (MQ=255)                              gTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCaa                                                                                                 >  2:642052/1‑140 (MQ=255)                                    cATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCt                                                                                           >  2:539132/1‑140 (MQ=255)                                        ttCGCGTCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTgcgg                                                                                       <  1:995964/140‑1 (MQ=255)                                                 aCCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAAt                                                                              >  2:220145/1‑140 (MQ=255)                                                                gTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGTGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAAtttt                                                               >  1:888675/1‑140 (MQ=255)                                                                 ttGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTa                                                              <  1:649552/140‑1 (MQ=255)                                                                      tCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATa                                                         <  1:642052/140‑1 (MQ=255)                                                                       ccATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATAc                                                        <  1:808119/140‑1 (MQ=255)                                                                           ataCTCGAAGTTGTGACAGTTACGGCACTTCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGct                                                    >  1:806370/1‑140 (MQ=255)                                                                           ataCTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATAc                                                        >  1:907946/1‑136 (MQ=255)                                                                           ataCTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATAc                                                        <  2:907946/136‑1 (MQ=255)                                                                           ataCTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGct                                                    >  1:954420/1‑140 (MQ=255)                                                                           ataCTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGct                                                    >  1:747650/1‑140 (MQ=255)                                                                           ataCTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGct                                                    >  1:548208/1‑140 (MQ=255)                                                                               tCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATa                                                         >  2:795072/1‑131 (MQ=255)                                                                               tCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATa                                                         <  1:795072/131‑1 (MQ=255)                                                                               tCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCttt                                                >  1:865429/1‑140 (MQ=255)                                                                                      tgtgACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATa                                                         <  2:977601/124‑1 (MQ=255)                                                                                      tgtgACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATa                                                         >  1:977601/1‑124 (MQ=255)                                                                                      tgtgACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCt                                         >  1:52975/1‑140 (MQ=255)                                                                                           cAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCTTTGAg                                    <  2:548208/140‑1 (MQ=255)                                                                                            aGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCTTTGAGc                                   <  1:372905/140‑1 (MQ=255)                                                                                            aGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCTTTGAGc                                   <  2:954420/140‑1 (MQ=255)                                                                                             gTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCTTTGAGCt                                  <  1:220145/140‑1 (MQ=255)                                                                                                      cTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCTTTGAGCTTGCGTatca                         >  2:441401/1‑140 (MQ=255)                                                                                                           gCGAGTTATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCTTTGAGCTTGCGTATCATCTTc                    <  1:1005448/140‑1 (MQ=255)                                                                                                                 tATTGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCACTTTACTTGCTTTGAGCTTGCGTATCATCTTCGGcaca              >  2:853855/1‑140 (MQ=255)                                                                                                                   ttGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCTTTGAGCTTGCGTATCATCTTCGGCACaaa            <  1:539132/140‑1 (MQ=255)                                                                                                                   ttGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCTTTGAGCTTGCGTATCATCTTCGGCACaaa            <  1:441401/140‑1 (MQ=255)                                                                                                                   ttGTCCTTCATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCTTTGAGCTTGCGTATCATCTTCGGCACaaa            <  2:865429/140‑1 (MQ=255)                                                                                                                           cATGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCTTTGAGCTTGCGTATCATCTTCGGCACAAACTCGTGCg    >  1:462381/1‑140 (MQ=255)                                                                                                                             tGCGCCGCCACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCTTTGAGCTTGCGTATCATCTTCGGGACAAACTCGTGCGGa  >  2:502034/1‑140 (MQ=255)                                                                                                                                    ccACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCTTTGAGCTTGCGTATCATCTTCGGCACAAACTCGTGCg    >  1:727227/1‑131 (MQ=255)                                                                                                                                    ccACCCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCTTTGAGCTTGCGTATCATCTTCGGCACAAACTCGTGCg    <  2:727227/131‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 ATACAGGTTTGCCCATCTTTCACCGCCTGGTCATGCATCTTCGCGGCAACCGATTTCTGGGCGGTTGTATCCATATACTCGAAGTTGTGACAGTTACGGCACTCCTGCGAGTTATTGTCCTTCATGCGCCGCCACTCATTCTGTGCCATCGTCAGACGATGAGCTTCAAATTTCTGCGGCGTGTCAATAACGCCAAAAATTTTACCATACAGCTCTTTACTTGCTTTGAGCTTGCGTATCATCTTCGGCACAAACTCGTGCGGA  >  NC_000913/2297721‑2297984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑