Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,355,854	G→A	V112I (GTC→ATC)	glpC →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,355,854	0	G	A	100.0%	163.3 / NA	47	V112I (GTC→ATC)	glpC	anaerobic glycerol‑3‑phosphate dehydrogenase subunit C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/19);  total (28/19)

CCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGC  >  NC_000913/2355719‑2355990                                                                                                                                        |                                                                                                                                         ccGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCaa                                                                                                                                      <  2:940238/140‑1 (MQ=255) ccGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCaa                                                                                                                                      <  1:79875/140‑1 (MQ=255)   gTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAaca                                                                                                                                    >  2:429264/1‑140 (MQ=255)                  tGGCGATATTATCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGa                                                                                                                     >  1:120119/1‑140 (MQ=255)                                    cgcgcgGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAgctgc                                                                                                   >  1:785608/1‑140 (MQ=255)                                      cgcgGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCtt                                                                                                 >  2:965009/1‑140 (MQ=255)                                      cgcgGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCtt                                                                                                 >  1:96836/1‑140 (MQ=255)                                       gcgGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTg                                                                                                >  2:313276/1‑140 (MQ=255)                                       gcgGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTg                                                                                                >  1:474456/1‑140 (MQ=255)                                          ggCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATg                                                                                             <  1:313276/140‑1 (MQ=255)                                            cGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATgcg                                                                                           <  2:474456/140‑1 (MQ=255)                                                 tatGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGtt                                                                                      <  2:96836/140‑1 (MQ=255)                                                         cacGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCg                                                                              >  2:268467/1‑140 (MQ=255)                                                          acGCCCCCGTCGCTGCGTATTTTTGCGTTGTGCCATACCGACCTGAGGGGGACCGTTTCCACGCCGTCCACACCAATCATCAACACCGCTACCTCGCTGAAACCGACGCGGCAGACGCTTGATGCGGCGTTAAAAATCGa                                                                             >  2:448579/1‑140 (MQ=255)                                                           cgcgCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGat                                                                            >  2:381326/1‑140 (MQ=255)                                                           cgcgCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGat                                                                            >  2:394311/1‑140 (MQ=255)                                                           cgcgCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGat                                                                            >  1:382509/1‑140 (MQ=255)                                                           cgcgCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGat                                                                            >  1:354850/1‑140 (MQ=255)                                                           cgcgCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGat                                                                            >  1:18397/1‑140 (MQ=255)                                                                ccGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATcg                                                                       >  2:408081/1‑140 (MQ=255)                                                                  gTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATcgcc                                                                     <  1:524152/140‑1 (MQ=255)                                                                   tCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATcgccg                                                                    >  2:443892/1‑140 (MQ=255)                                                                   tCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATcgccg                                                                    <  1:283849/140‑1 (MQ=255)                                                                     gCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCa                                                                  <  1:612198/140‑1 (MQ=255)                                                                     gCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCa                                                                  <  1:443892/140‑1 (MQ=255)                                                                     gCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCa                                                                  <  2:120119/140‑1 (MQ=255)                                                                                   tgtTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATAGCCGCACGCTACCGAAATAc                                                    <  2:785608/140‑1 (MQ=255)                                                                                           cATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCgg                                            <  1:381326/140‑1 (MQ=255)                                                                                             tACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCa                                          <  1:965009/140‑1 (MQ=255)                                                                                                  aCCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTc                                     >  2:644006/1‑140 (MQ=255)                                                                                                  aCCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTc                                     >  1:701086/1‑140 (MQ=255)                                                                                                    cTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCg                                   >  2:985694/1‑140 (MQ=255)                                                                                                       aTGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCa                                          >  1:7854/1‑130 (MQ=255)                                                                                                       aTGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCa                                          <  2:7854/130‑1 (MQ=255)                                                                                                        tGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGc                               >  1:108316/1‑140 (MQ=255)                                                                                                        tGGGTAGCGTTTCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGc                               <  2:701086/140‑1 (MQ=255)                                                                                                                 tttCCACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGc                      >  2:929000/1‑140 (MQ=255)                                                                                                                    ccACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGc                   >  2:376239/1‑140 (MQ=255)                                                                                                                    ccACGCCGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGc                   >  1:1004125/1‑140 (MQ=255)                                                                                                                         ccGTTCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGATATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTggc              >  1:240309/1‑140 (MQ=255)                                                                                                                            ttCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTggcggc           <  1:985694/140‑1 (MQ=255)                                                                                                                            ttCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTggcggc           <  1:949007/140‑1 (MQ=255)                                                                                                                            ttCGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTggcggc           >  1:138845/1‑140 (MQ=255)                                                                                                                              cGCACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTc         >  1:901357/1‑140 (MQ=255)                                                                                                                                cACCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCag       <  2:354850/140‑1 (MQ=255)                                                                                                                                 aCCAATCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCagc      <  1:343316/140‑1 (MQ=255)                                                                                                                                    aaTCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCagcaag   >  2:387620/1‑140 (MQ=255)                                                                                                                                    aaTCATCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCagcaag   >  2:119877/1‑140 (MQ=255)                                                                                                                                     atcatcAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCagcaagc  <  1:644006/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CCGTCCGATGTGAAGATTGGCGATATTATCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGC  >  NC_000913/2355719‑2355990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑