| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,370,617 | G→A | W200* (TGG→TGA) | arnD → | putative 4‑deoxy‑4‑formamido‑L‑arabinose‑phosphoundecaprenol deformylase ArnD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,370,617 | 0 | G | A | 98.0% | 167.0 / ‑4.6 | 49 | W200* (TGG→TGA) | arnD | putative 4‑deoxy‑4‑formamido‑L‑arabinose‑phosphoundecaprenol deformylase ArnD |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (22/26); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGAAGGTGATCGAAGCAAAAGAAGCGTTCCATTTGCGCTACAACAGCGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGGGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTG > NC_000913/2370482‑2370753 | ccAGAAGGTGATCGAAGCAAAAGAAGCGTTCCATTTGCGCTACAACAGCGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATg < 2:938467/140‑1 (MQ=255) aTCGAAGCAAAAGAAGCGTTCCATTTGCGCTACAACAGCGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTgg > 2:308807/1‑140 (MQ=255) tCGAAGCAAAAGAAGCGTTCCATTTGCGCTACAACAGCGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATg > 2:42631/1‑129 (MQ=255) tCGAAGCAAAAGAAGCGTTCCATTTGCGCTACAACAGCGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATg < 1:42631/129‑1 (MQ=255) tCGAAGCAAAAGAAGCGTTCCATTTGCGCTACAACAGCGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGt > 1:207849/1‑140 (MQ=255) tCGAAGCAAAAGAAGCGTTCCATTTGCGCTACAACAGCGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGt < 2:47899/140‑1 (MQ=255) aaGCAAAAGAAGCGTTCCATTTGCGCTACAACAGCGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCgg > 2:958773/1‑140 (MQ=255) gTTCCATTTGCGCTACAACAGCGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCag > 2:962559/1‑140 (MQ=255) gTTCCATTTGCGCTACAACAGCGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCag > 1:296372/1‑140 (MQ=255) aTTTGCGCTACAACAGCGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGAt > 1:514869/1‑140 (MQ=255) tttGCGCTACAACAGCGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGAtt > 1:859479/1‑140 (MQ=255) cTACAACAGCGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACg < 2:483525/140‑1 (MQ=255) caacaGCGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACggtt < 1:217710/140‑1 (MQ=255) cGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCCGGCACTGCGCAAATTCCGGTGACCCTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTAc > 1:12141/1‑140 (MQ=255) aTTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTc > 1:344056/1‑140 (MQ=255) aTTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTc > 2:281755/1‑140 (MQ=255) gTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAAc > 1:352543/1‑122 (MQ=255) gTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAAc < 2:352543/122‑1 (MQ=255) tGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGTACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCa < 1:168334/140‑1 (MQ=255) ggggCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCAt > 1:690759/1‑140 (MQ=255) gggCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATc < 2:296372/140‑1 (MQ=255) cATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGc < 1:962559/140‑1 (MQ=255) ccttGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACg < 2:690759/138‑1 (MQ=255) aTTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGc < 2:264066/140‑1 (MQ=255) cTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGt < 1:116874/140‑1 (MQ=255) cTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGACAAAGGCACGCCGGt < 2:859479/140‑1 (MQ=255) gAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTta > 2:679689/1‑140 (MQ=255) ggAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCAt < 1:679689/140‑1 (MQ=255) gAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACg < 2:916231/125‑1 (MQ=255) gAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACg > 1:916231/1‑125 (MQ=255) aaaCCCCGGCACTGCGCAAATTCCGGTGACCCTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCA‑‑‑CGGTTTATACCATTCATg < 2:12141/140‑1 (MQ=255) cccTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATg < 1:944506/140‑1 (MQ=255) ccTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGc < 1:281755/140‑1 (MQ=255) tGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAg < 1:958773/140‑1 (MQ=255) gCACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGaa > 1:60318/1‑140 (MQ=255) cACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAg > 1:704512/1‑137 (MQ=255) cACTGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAg < 2:704512/137‑1 (MQ=255) tGCGCAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTg < 2:514869/140‑1 (MQ=255) cgcAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAg < 2:542380/132‑1 (MQ=255) cgcAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAg > 1:542380/1‑132 (MQ=255) cgcAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAg > 2:369998/1‑132 (MQ=255) cgcAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAg < 1:369998/132‑1 (MQ=255) cgcAAATTCCGGTGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGaa > 2:77715/1‑140 (MQ=255) tGACCTTACCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTAt < 1:308807/140‑1 (MQ=255) aCCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCGCGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATa < 2:207849/140‑1 (MQ=255) aCCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATa > 2:419246/1‑140 (MQ=255) aCCCACCTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTT‑GTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATaa < 2:368370/140‑1 (MQ=255) ccTGAGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAAtttt > 2:126583/1‑140 (MQ=255) cTGGGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTtg > 2:647594/1‑140 (MQ=255) | CCAGAAGGTGATCGAAGCAAAAGAAGCGTTCCATTTGCGCTACAACAGCGATTGTCGTGGGGCCATGCCGTTCCGTCCATTGCTCGAATCAGGAAACCCTGGCACTGCGCAAATTCCGGTGACCTTACCCACCTGGGATGAAGTGATTGGTCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTG > NC_000913/2370482‑2370753 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |