Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,448,685	A→G	Q27R (CAG→CGG)	smrB →	putative endonuclease SmrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,448,685	0	A	G	100.0%	180.8 / NA	52	Q27R (CAG→CGG)	smrB	putative endonuclease SmrB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/30);  total (22/30)

ATCGATTGCGCTACGAGTCGGGTAAACTGTAGGAAAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGG  >  NC_000913/2448549‑2448819                                                                                                                                         |                                                                                                                                       atcgatTGCGCTACGAGTCGGGTAAACTGTAGGAAAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGa                                                                                                                                     >  1:330732/1‑140 (MQ=255) atcgatTGCGCTACGAGTCGGGTAAACTGTAGGAAAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGa                                                                                                                                     <  2:865077/140‑1 (MQ=255)   cgatTGCGCTACGAGTCGGGTAAACTGTAGGAAAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGaca                                                                                                                                   <  2:330732/140‑1 (MQ=255)   cgatTGCGCTACGAGTCGGGTAAACTGTAGGAAAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGaca                                                                                                                                   <  2:786360/140‑1 (MQ=255)                              tAGGAAAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTaaaa                                                                                                        >  1:601347/1‑140 (MQ=255)                                  aaaaTTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAAt                                                                                                    <  2:28157/140‑1 (MQ=255)                                      ttAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGc                                                                                                >  1:937613/1‑140 (MQ=255)                                                      aTAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAAc                                                                                >  2:920332/1‑140 (MQ=255)                                                         aaTGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCt                                                                             <  2:488590/140‑1 (MQ=255)                                                         aaTGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCt                                                                             <  2:116105/140‑1 (MQ=255)                                                          aTGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCtt                                                                            <  1:763490/140‑1 (MQ=255)                                                               aaaGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCt                                                                             >  1:1003666/1‑134 (MQ=255)                                                               aaaGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCt                                                                             <  2:1003666/134‑1 (MQ=255)                                                                aaGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCa                                                                      >  2:192831/1‑140 (MQ=255)                                                                    aaaCAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAg                                                                  <  1:848543/140‑1 (MQ=255)                                                                    aaaCAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAg                                                                  <  2:365896/140‑1 (MQ=255)                                                                    aaaCAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAg                                                                  <  1:427722/140‑1 (MQ=255)                                                                     aacaacACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCt                                                                             >  1:808010/1‑128 (MQ=255)                                                                     aacaacACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCt                                                                             <  2:808010/128‑1 (MQ=255)                                                                               aGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGGAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATgc                                                       >  2:891141/1‑140 (MQ=255)                                                                               aGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATgc                                                       <  1:920332/140‑1 (MQ=255)                                                                                     gtggaCCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGAAAGATTAAACGGGACACGAATGTCCACCGACCGCACCGTAAAAAAATCAGCGAAGAGCCGGACAAACGCTTGATCCAAGAGCAGGCTGATgccagcca                                                 >  2:111190/3‑140 (MQ=255)                                                                                     gaggaCCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATgccagcca                                                 >  2:551747/1‑140 (MQ=255)                                                                                     gaggaCCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATgccagcca                                                 >  2:58527/1‑140 (MQ=255)                                                                                         aCCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCattat                                             >  1:778082/1‑140 (MQ=255)                                                                                          ccAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCattatt                                            >  2:584342/1‑140 (MQ=255)                                                                                             ggCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTtct                                         <  1:584342/140‑1 (MQ=255)                                                                                                tctGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATgcca                                                     <  1:424482/125‑1 (MQ=255)                                                                                                tctGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATgcca                                                     >  2:424482/1‑125 (MQ=255)                                                                                                  tGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGAt                                    >  2:15006/1‑140 (MQ=255)                                                                                                  tGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGAt                                    >  1:899099/1‑140 (MQ=255)                                                                                                  tGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGAt                                    >  1:787624/1‑140 (MQ=255)                                                                                                  tGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGAt                                    >  1:766572/1‑140 (MQ=255)                                                                                                   gTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATg                                   >  1:977484/1‑140 (MQ=255)                                                                                                         ccAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTc                             <  2:937613/140‑1 (MQ=255)                                                                                                         ccAGTTGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTc                             <  1:111190/140‑1 (MQ=255)                                                                                                              tGATGGCGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCg                        <  2:899099/140‑1 (MQ=255)                                                                                                                   gCGGGGACTCGGAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGttatt                   <  1:376944/140‑1 (MQ=255)                                                                                                                    cGGGGACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGttatta                  <  2:766572/140‑1 (MQ=255)                                                                                                                      gggACTCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTaaa                >  1:820371/1‑140 (MQ=255)                                                                                                                           tCGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCg           <  2:820371/140‑1 (MQ=255)                                                                                                                            cGCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGa          <  1:1008760/140‑1 (MQ=255)                                                                                                                             gCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCg                        >  2:805461/1‑125 (MQ=255)                                                                                                                             gCAAGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCg                        <  1:805461/125‑1 (MQ=255)                                                                                                                               aaGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAgg       <  1:15006/140‑1 (MQ=255)                                                                                                                                aGATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGt      <  1:551747/140‑1 (MQ=255)                                                                                                                                 gATTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTc     >  2:18473/1‑140 (MQ=255)                                                                                                                                  aTTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTcc    <  2:601347/140‑1 (MQ=255)                                                                                                                                  aTTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTcc    <  1:891141/140‑1 (MQ=255)                                                                                                                                  aTTAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTcc    <  1:58527/140‑1 (MQ=255)                                                                                                                                    tAAGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCgg  <  2:962689/140‑1 (MQ=255)                                                                                                                                      aGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTcc    <  2:700292/136‑1 (MQ=255)                                                                                                                                      aGCGGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTcc    >  1:700292/1‑136 (MQ=255)                                                                                                                                         |                                                                                                                                       ATCGATTGCGCTACGAGTCGGGTAAACTGTAGGAAAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTTAGCGAGGAGGACCAGGCTCTGTTTCGCCAGTTGATGGCGGGGACTCGCAAGATTAAGCAGGACACGATTGTCCACCGACCGCAGCGTAAAAAAATCAGCGAAGTGCCGGTGAAACGCTTGATCCAGGAGCAGGCTGATGCCAGCCATTATTTCTCCGATGAGTTTCAGCCGTTATTAAATACCGAAGGTCCGG  >  NC_000913/2448549‑2448819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑