Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,544,253	A→G	V124A (GTG→GCG)	ucpA ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,544,253	0	A	G	100.0%	166.9 / NA	48	V124A (GTG→GCG)	ucpA	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/30);  total (18/30)

TTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTT  >  NC_000913/2544124‑2544388                                                                                                                                  |                                                                                                                                        tttGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGt                                                                                                                               >  2:651419/1‑140 (MQ=255)  ttGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTg                                                                                                                              <  2:997102/140‑1 (MQ=255)  ttGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTg                                                                                                                              <  2:872061/140‑1 (MQ=255)     tCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACg                                                                                                                           <  1:358107/140‑1 (MQ=255)         gCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTcc                                                                                                                       >  2:464383/1‑140 (MQ=255)            aacaaTCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATa                                                                                                                    <  2:643298/140‑1 (MQ=255)            aacaaTCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATa                                                                                                                    <  2:586502/140‑1 (MQ=255)            aacaaTCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATa                                                                                                                    <  1:1009460/140‑1 (MQ=255)             acaaTCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATTATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATAc                                                                                                                   <  1:889319/140‑1 (MQ=255)             acaaTCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATAc                                                                                                                   <  1:319832/140‑1 (MQ=255)             acaaTCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATAc                                                                                                                   <  1:651419/140‑1 (MQ=255)             acaaTCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATAc                                                                                                                   <  1:756260/140‑1 (MQ=255)              caaTCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACg                                                                                                                  >  2:345432/1‑140 (MQ=255)                aTCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGcc                                                                                                                <  1:345432/140‑1 (MQ=255)                  cgccgcTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCtt                                                                                                              <  1:204323/140‑1 (MQ=255)                            gTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGAt                                                                                                    <  1:287502/140‑1 (MQ=255)                                      aCGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATgg                                                                                          >  1:993615/1‑140 (MQ=255)                                                gCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGAt                                                                                <  1:957869/140‑1 (MQ=255)                                                    ggATCGGCCACCATATCATCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTc                                                                            <  1:464383/140‑1 (MQ=255)                                                    ggATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTc                                                                            <  2:260039/140‑1 (MQ=255)                                                      aTCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCAt                                                                          >  2:125177/1‑140 (MQ=255)                                                          gCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCAACGCt                                                                      <  2:492654/140‑1 (MQ=255)                                                           ccaccaTATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTACGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTc                                                                     <  2:742710/140‑1 (MQ=255)                                                             accaTATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCat                                                                   <  2:675055/140‑1 (MQ=255)                                                                 tatCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCg                                                               >  1:912695/1‑140 (MQ=255)                                                                         gTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCg                                                                        >  2:522757/1‑123 (MQ=255)                                                                         gTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCg                                                                        <  1:522757/123‑1 (MQ=255)                                                                         gTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGa                                                              <  1:65610/133‑1 (MQ=255)                                                                         gTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGa                                                              >  2:65610/1‑133 (MQ=255)                                                                         gTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACt                                                       >  1:910993/1‑140 (MQ=255)                                                                              tGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCa                                                  >  1:208405/1‑140 (MQ=255)                                                                                    catcatCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGcc                                                    >  1:581652/1‑132 (MQ=255)                                                                                    catcatCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGcc                                                    <  2:581652/132‑1 (MQ=255)                                                                                     atcatcACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACa                                           >  1:310085/1‑140 (MQ=255)                                                                                       catcaCAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCGGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACaaa                                         >  2:701128/1‑140 (MQ=255)                                                                                        atcaCAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAAc                                        >  1:458336/1‑140 (MQ=255)                                                                                          cacaATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGc                                      >  2:25859/1‑140 (MQ=255)                                                                                            caATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCt                                    <  2:993615/140‑1 (MQ=255)                                                                                                  gACCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTa                              <  2:458336/140‑1 (MQ=255)                                                                                                   aCCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTAt                             >  2:336158/1‑140 (MQ=255)                                                                                                   aCCATCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTAt                             >  1:686912/1‑140 (MQ=255)                                                                                                       tCTTTGCGGGCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCAc                         <  2:113462/140‑1 (MQ=255)                                                                                                               ggCAATCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGatat                 <  1:662368/140‑1 (MQ=255)                                                                                                                  aaTCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCAc                         <  2:190844/129‑1 (MQ=255)                                                                                                                  aaTCATCTCCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCAc                         >  1:190844/1‑129 (MQ=255)                                                                                                                       tctcCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATgcgc         <  2:686912/140‑1 (MQ=255)                                                                                                                        ctcCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGcc        <  2:688706/140‑1 (MQ=255)                                                                                                                        ctcCGGCAGCGCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGcc        <  1:896874/140‑1 (MQ=255)                                                                                                                              cagcgCCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCcttctt  >  1:561958/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        TTTGTCAGGCCAACAATCGCCGCTTTCGTTAAGGCGTACGCCGTTTCGCCAGGATCGGCCACCATATCACCAGTGACTGAAGACATCATCACAATGCGACCATCTTTGCGGGCAATCATCTCCGGCAGCACCGCCTTCGTGACGTTCCATACGCCTTTAATATTGATGTCAATATGGAAATCGCGATCGTCATCGCTCATATCGAGGAAACTGCCCAGACGACAAACGCCTGCGTTATTCACCAGGATATCAATGCGCCCTTCTT  >  NC_000913/2544124‑2544388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑