Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,567,131	T→C	N326S (AAC→AGC)	eutH ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,567,131	0	T	C	100.0%	180.8 / NA	51	N326S (AAC→AGC)	eutH	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/24);  total (27/24)

CATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGTTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGATACGCCCCTAACAGAACGCAGG  >  NC_000913/2567001‑2567266                                                                                                                                   |                                                                                                                                        cATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCaa                                                                                                                                >  2:777165/1‑140 (MQ=255)  aTGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAg                                                                                                                               <  1:360816/140‑1 (MQ=255)        ttCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGc                                                                                                                         >  1:377320/1‑140 (MQ=255)         tCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGcc                                                                                                                        >  2:923582/1‑140 (MQ=255)         tCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGcc                                                                                                                        >  2:763952/1‑140 (MQ=255)          cATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGcca                                                                                                                       >  1:47164/1‑140 (MQ=255)            tGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGccacc                                                                                                                     <  2:47164/140‑1 (MQ=255)                     cggcgAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCt                                                                                                            >  2:13236/1‑140 (MQ=255)                           aGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCcgcg                                                                                                      <  1:923582/140‑1 (MQ=255)                               tAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGAtgt                                                                                                  <  1:763952/140‑1 (MQ=255)                               tAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGAtgt                                                                                                  <  2:204064/140‑1 (MQ=255)                                     gTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCa                                                                                            >  1:169482/1‑140 (MQ=255)                                       cGCCCAGGGCAAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGAt                                                                                                    >  2:350029/1‑130 (MQ=255)                                       cGCCCAGGGCAAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGAt                                                                                                    <  1:350029/130‑1 (MQ=255)                                            aGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAg                                                                                     <  1:710958/140‑1 (MQ=255)                                             gggCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGt                                                                                    >  1:587703/1‑140 (MQ=255)                                             gggCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGt                                                                                    >  2:474291/1‑140 (MQ=255)                                             gggCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGAATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGt                                                                                    >  1:387486/1‑140 (MQ=255)                                                    agcagcGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTAc                                                                             <  2:975134/140‑1 (MQ=255)                                                      cagcGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCg                                                                           <  2:587703/140‑1 (MQ=255)                                                        gcGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGAc                                                                         >  1:788462/1‑140 (MQ=255)                                                                          cAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTGTc                                                       >  2:624586/1‑140 (MQ=255)                                                                                  gaCTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGc                                               <  1:624586/140‑1 (MQ=255)                                                                                     tttGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAg                                            <  1:13236/140‑1 (MQ=255)                                                                                     tttGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAg                                            <  1:279040/140‑1 (MQ=255)                                                                                       tGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTc                                          <  1:761455/140‑1 (MQ=255)                                                                                       tGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTc                                          <  1:244615/140‑1 (MQ=255)                                                                                       tGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTc                                          >  2:244615/1‑140 (MQ=255)                                                                                                 aTCCATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAgcagcagca                                <  2:788462/140‑1 (MQ=255)                                                                                                    cATCTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCa                             <  1:474291/140‑1 (MQ=255)                                                                                                       cTGCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCACCg                          >  1:860309/1‑140 (MQ=255)                                                                                                         gCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTaagcagcag                                  >  2:875162/1‑130 (MQ=255)                                                                                                         gCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTaagcagcag                                  <  1:875162/130‑1 (MQ=255)                                                                                                         gCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCa                             <  1:357547/135‑1 (MQ=255)                                                                                                         gCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCa                             >  2:357547/1‑135 (MQ=255)                                                                                                         gCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGa                        >  1:311494/1‑140 (MQ=255)                                                                                                         gCTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTAAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGa                        >  2:529147/1‑140 (MQ=255)                                                                                                          cTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGAt                       <  2:169482/140‑1 (MQ=255)                                                                                                          cTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGAt                       <  1:60001/140‑1 (MQ=255)                                                                                                          cTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGAt                       >  1:931181/1‑140 (MQ=255)                                                                                                          cTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGAt                       <  1:777165/140‑1 (MQ=255)                                                                                                          cTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGAt                       >  2:60001/1‑140 (MQ=255)                                                                                                          cTTCATCATGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGAt                       >  2:414147/1‑140 (MQ=255)                                                                                                             catcatGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGATACg                    >  1:979362/1‑140 (MQ=255)                                                                                                               tcatGCCGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGATACGcc                  >  2:509326/1‑140 (MQ=255)                                                                                                                    ccGAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTAAAACCAGCGAGTCAGCAGCAGCACCATCGGATACGCCCCTaa             >  2:188280/1‑140 (MQ=255)                                                                                                                      gAACATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGATACGCCCCTAACa           <  2:931181/140‑1 (MQ=255)                                                                                                                        aCATCGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTAAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGATACGCCCCTAACAGa         <  1:529147/140‑1 (MQ=255)                                                                                                                            cGGGATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGATACGCCCCTAACAGAACGc     >  1:849908/1‑140 (MQ=255)                                                                                                                             gggATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTAAAACCAGCGAGTCAGCAGCAGCACCATCGGATACGCCCCTAACAGAACGCa    <  1:188280/140‑1 (MQ=255)                                                                                                                               gATGCTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGATACGCCCCTAACAGAACGCAgg  >  1:186259/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        CATGGCGTTCATGTTGGCAGCGGCGAAGCCTAAGTGGTCGCCCAGGGCGAAAGCAGCGGAAACGGCGAAGGCGCAGTTGATGACTTTGCCGCGGGTATCCATCTGCTTCATCATGCCGAACATCGGGATGTTGTTGGCAAGCGTTGCCACCATGCCGGCTGCCGCGATGTTGTTCATATTCAGTACTTTACCGACGCTCATCAGCGGTTTTTCAAACCAGCGAGTCAGCAGCAGCACCATCGGATACGCCCCTAACAGAACGCAGG  >  NC_000913/2567001‑2567266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑