Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,612,257	T→C	F120L (TTT→CTT)	hyfR →	DNA‑binding transcriptional activator HyfR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,612,257	0	T	C	100.0%	243.2 / NA	68	F120L (TTT→CTT)	hyfR	DNA‑binding transcriptional activator HyfR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/35);  total (33/35)

TGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAG  >  NC_000913/2612120‑2612389                                                                                                                                          |                                                                                                                                     tgACGGCAACAACAGAGTATCAGGGCGAGATCGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGGTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGctt                                                                                                                                    >  2:454294/1‑140 (MQ=255)   aCGGCAACAACAGAGTATCAGGGCGAGATGGTCTATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTaa                                                                                                                                  >  1:348360/1‑140 (MQ=255)         acaacaGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAActggct                                                                                                                            >  2:1004300/1‑140 (MQ=255)           aacaGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAActggctgg                                                                                                                          <  2:267244/140‑1 (MQ=255)                       gggCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGTGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCa                                                                                                              >  2:199770/1‑140 (MQ=255)                          cGAGATGGTCTATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCAccc                                                                                                           <  2:348360/140‑1 (MQ=255)                          cGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCAccc                                                                                                           >  2:279048/1‑140 (MQ=255)                          cGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCAccc                                                                                                           >  1:658546/1‑140 (MQ=255)                          cGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCAccc                                                                                                           <  1:665572/140‑1 (MQ=255)                          cGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCAccc                                                                                                           <  2:788127/140‑1 (MQ=255)                          cGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCAccc                                                                                                           <  1:1004300/140‑1 (MQ=255)                            agaTGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCCCGGCAGTCGTTTTACTTCCCCGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCAccccc                                                                                                         >  2:770583/1‑139 (MQ=255)                            agaTGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCg                                                                                                         >  2:38634/1‑140 (MQ=255)                            agaTGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCg                                                                                                         >  1:449474/1‑140 (MQ=255)                             gaTGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCgg                                                                                                        <  1:72482/140‑1 (MQ=255)                              aTGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGACTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGt                                                                                                       >  1:261593/1‑140 (MQ=255)                                       tattCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATc                                                                                              >  2:952126/1‑140 (MQ=255)                                           cgcgTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATgg                                                                                          >  2:595683/1‑140 (MQ=255)                                           cgcgTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATgg                                                                                          >  1:224142/1‑140 (MQ=255)                                            gcgTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGc                                                                                         >  1:236362/1‑140 (MQ=255)                                            gcgTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCG‑TTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCt                                                                                        >  2:836352/1‑140 (MQ=255)                                             cgTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCt                                                                                        <  1:532818/140‑1 (MQ=255)                                             cgTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCt                                                                                        <  1:595683/140‑1 (MQ=255)                                              gTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTc                                                                                       >  1:145469/1‑140 (MQ=255)                                                cACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCaa                                                                                     <  1:38634/140‑1 (MQ=255)                                                   gCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAatga                                                                                  >  2:695784/1‑140 (MQ=255)                                                       tCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATgaa                                                                              <  1:803290/140‑1 (MQ=255)                                                                 cAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAgg                                                                    <  2:236362/140‑1 (MQ=255)                                                                            tGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGt                                                         <  1:1012336/140‑1 (MQ=255)                                                                            tGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGt                                                         <  1:952126/140‑1 (MQ=255)                                                                              gCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGttt                                                       >  2:308977/1‑140 (MQ=255)                                                                              gCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATCGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGttt                                                       >  1:517970/1‑140 (MQ=255)                                                                               tggAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTg                                                      <  1:199770/139‑1 (MQ=255)                                                                                 gAGCTACGCTTTCTTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCt                                                    >  1:643795/1‑140 (MQ=255)                                                                                      aCGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGgaga                                               <  2:376807/140‑1 (MQ=255)                                                                                            tATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATc                                         >  2:203978/1‑140 (MQ=255)                                                                                                cGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGa                                     <  2:216061/140‑1 (MQ=255)                                                                                                 gCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGat                                    >  1:954132/1‑140 (MQ=255)                                                                                                  cACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGatc                                   >  2:772865/1‑140 (MQ=255)                                                                                                   aCCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGatca                                  <  1:64036/140‑1 (MQ=255)                                                                                                   aCCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGatca                                  <  1:680023/140‑1 (MQ=255)                                                                                                   aCCCACGGCAGTCGTTTTACTTCCCAGGATCGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGatca                                  <  2:517970/140‑1 (MQ=255)                                                                                                       aCGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGatcatcat                              <  2:643795/140‑1 (MQ=255)                                                                                                       aCGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGatcatcat                              <  1:279048/140‑1 (MQ=255)                                                                                                       aCGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGatcatcat                              <  1:695784/140‑1 (MQ=255)                                                                                                        cGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGatcatcatc                             >  2:278587/1‑140 (MQ=255)                                                                                                        cGGCAGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGatcatcatc                             >  2:341994/1‑140 (MQ=255)                                                                                                            aGTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGt                         <  1:341994/140‑1 (MQ=255)                                                                                                            aGTCG‑TTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTa                        <  1:836352/140‑1 (MQ=255)                                                                                                             gTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTa                        <  1:772865/140‑1 (MQ=255)                                                                                                             gTCGTTTTACTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTa                        >  2:580354/1‑140 (MQ=255)                                                                                                                     aCTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGa                >  1:851437/1‑140 (MQ=255)                                                                                                                     aCTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGa                <  2:684460/140‑1 (MQ=255)                                                                                                                      cTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGat               <  2:233163/140‑1 (MQ=255)                                                                                                                      cTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGat               <  1:65405/140‑1 (MQ=255)                                                                                                                      cTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGat               <  1:278587/140‑1 (MQ=255)                                                                                                                      cTTCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATCGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCAACATCGGGTACTGGTTGat               <  1:454294/140‑1 (MQ=255)                                                                                                                      cTTCCCAGGACTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGat               <  2:261593/140‑1 (MQ=255)                                                                                                                        tCCCAGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGatat             <  2:658546/140‑1 (MQ=255)                                                                                                                            aGGATTGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACt         >  2:311308/1‑140 (MQ=255)                                                                                                                                ttGCACACTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATg     <  2:685765/140‑1 (MQ=255)                                                                                                                                   cacaCTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGat               <  1:445920/127‑1 (MQ=255)                                                                                                                                   cacaCTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGat               >  2:445920/1‑127 (MQ=255)                                                                                                                                   cacaCTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATg     <  1:383751/137‑1 (MQ=255)                                                                                                                                   cacaCTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATg     >  2:383751/1‑137 (MQ=255)                                                                                                                                   cacaCTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAg  >  2:642495/1‑140 (MQ=255)                                                                                                                                   cacaCTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAg  >  1:479105/1‑140 (MQ=255)                                                                                                                                   cacaCTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAg  >  2:246582/1‑140 (MQ=255)                                                                                                                                   cacaCTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAg  >  1:291769/1‑140 (MQ=255)                                                                                                                                   cacaCTGCTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAg  >  2:369499/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     TGACGGCAACAACAGAGTATCAGGGCGAGATGGTCGATTATTCGCGTCACGCCGTCCTTCTCGGCAGTAGTGGAATGGCGGAGCTACGCTTTATTCGCACCCACGGCAGTCGTTTTACTTCCCAGGATTGCACACTGTTTAACTGGCTGGCGCGGATAATCACCCCGGTTCTGCAATCATGGCTCAATGATGAAGAACAGCAGGTGGCGCTGCGTTTGCTGGAGAAAGATCGCGATCATCATCGGGTACTGGTTGATATTACTAATGCAG  >  NC_000913/2612120‑2612389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑