Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	209,624	(T)6→5	intergenic (+44/‑55)	accA → / → ldcC	acetyl‑CoA carboxyltransferase subunit alpha/lysine decarboxylase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	209,619	0	T	.	100.0%	298.5 / NA	71	intergenic (+39/‑60)	accA/ldcC	acetyl‑CoA carboxyltransferase subunit alpha/lysine decarboxylase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (32/39);  total (32/39)

AAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGGC  >  NC_000913/209480‑209752                                                                                                                                            |                                                                                                                                      aaGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCt                                                                                                                                       >  2:281568/1‑140 (MQ=255)      cAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCttttta                                                                                                                                  <  2:512238/140‑2 (MQ=255)           gctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGcc                                                                                                                            <  1:112893/140‑1 (MQ=255)                   aTCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTg                                                                                                                    >  1:995238/1‑140 (MQ=255)                   aTCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTg                                                                                                                    <  1:284023/140‑1 (MQ=255)                     cttgCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAg                                                                                                                  >  2:1137629/4‑140 (MQ=255)                     cTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAg                                                                                                                  <  1:179740/140‑1 (MQ=255)                     cTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAg                                                                                                                  <  1:642963/140‑1 (MQ=255)                     cTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAg                                                                                                                  <  1:1137629/140‑1 (MQ=255)                     cTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAg                                                                                                                  <  1:957997/140‑1 (MQ=255)                     cTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAg                                                                                                                  <  2:909162/140‑1 (MQ=255)                      tGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGc                                                                                                                 >  1:893578/1‑140 (MQ=255)                           gATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCt                                                                                                            <  2:144216/140‑1 (MQ=255)                           gATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCt                                                                                                            <  2:245535/140‑1 (MQ=255)                           gATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCt                                                                                                            <  1:968329/140‑1 (MQ=255)                           gATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCt                                                                                                            <  2:179291/140‑1 (MQ=255)                               tcGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGa                                                                                                        >  1:615924/1‑140 (MQ=255)                                  aCGTGTTAAGCACTGAAGATTTAGAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAg                                                                                                                  >  1:514799/1‑127 (MQ=255)                                  aCGTGTTAAGCACTGAAGATTTAGAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAg                                                                                                                  <  2:514799/127‑1 (MQ=255)                                  aCGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAg                                                                                                                  >  2:868361/1‑127 (MQ=255)                                  aCGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAg                                                                                                                  <  1:868361/127‑1 (MQ=255)                                  aCGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTa                                                                                                     >  1:334804/1‑140 (MQ=255)                                         aaGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTaaggaagg                                                                                              >  2:330569/1‑140 (MQ=255)                                               tGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTaagg                                                                                                  <  1:496741/130‑1 (MQ=255)                                               tGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTaagg                                                                                                  >  2:496741/1‑130 (MQ=255)                                                 aaGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCa                                                                                      >  2:453506/1‑140 (MQ=255)                                                        aaaaaaTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGaa                                                                               <  2:334804/140‑1 (MQ=255)                                                         aaaaaTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTcc                                                                                       >  2:1028372/1‑131 (MQ=255)                                                         aaaaaTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTcc                                                                                       <  1:1028372/131‑1 (MQ=255)                                                              tcgtcgTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATg                                                                         >  2:150334/1‑140 (MQ=255)                                                                       tCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGGACACATGAACATCAtt                                                                >  1:897156/1‑140 (MQ=255)                                                                       tCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCAtt                                                                <  1:135056/140‑1 (MQ=255)                                                                         aGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGGACACATGAACATCATTGc                                                              <  2:897156/140‑1 (MQ=255)                                                                            gcCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCAt                                                           >  1:446132/1‑140 (MQ=255)                                                                            gcCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCAt                                                           >  2:334608/1‑140 (MQ=255)                                                                               tgatgaGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTAt                                                        >  1:838591/1‑140 (MQ=255)                                                                                gatgaGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAcat                                                                    >  1:1058022/1‑127 (MQ=255)                                                                                gatgaGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAcat                                                                    <  2:1058022/127‑1 (MQ=255)                                                                                 atgaGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGc                                                              <  2:416848/132‑1 (MQ=255)                                                                                 atgaGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGc                                                              >  1:416848/1‑132 (MQ=255)                                                                                 atgaGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATgg                                                      >  2:390297/1‑140 (MQ=255)                                                                                 atgaGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATgg                                                      >  2:908935/1‑140 (MQ=255)                                                                                 atgaGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATgg                                                      >  1:985184/1‑140 (MQ=255)                                                                                 atgaGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATgg                                                      >  2:60179/1‑140 (MQ=255)                                                                                      cTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCg                                                 >  1:935059/1‑140 (MQ=255)                                                                                        aCGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCa                                               >  1:448018/1‑140 (MQ=255)                                                                                          ggTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATg                                             <  2:243169/140‑1 (MQ=255)                                                                                          ggTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATg                                             <  2:446132/140‑1 (MQ=255)                                                                                           gTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGc                                                              <  2:997217/122‑1 (MQ=255)                                                                                           gTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGc                                                              >  1:997217/1‑122 (MQ=255)                                                                                           gTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATgg                                            >  2:444968/1‑140 (MQ=255)                                                                                            ttACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGc                                           <  2:995238/140‑1 (MQ=255)                                                                                            ttACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGc                                           <  1:390297/140‑1 (MQ=255)                                                                                              aCGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGt                                         <  2:893578/140‑1 (MQ=255)                                                                                               cgcgTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTc                                        <  1:453506/140‑1 (MQ=255)                                                                                               cgcgTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTc                                        <  1:334608/140‑1 (MQ=255)                                                                                                gcgTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTGAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGc                                           <  1:537571/136‑1 (MQ=255)                                                                                                gcgTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTGAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGc                                           >  2:537571/1‑136 (MQ=255)                                                                                                 cgTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGt                                         >  1:97323/1‑137 (MQ=255)                                                                                                 cgTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGt                                         <  2:97323/137‑1 (MQ=255)                                                                                                     aTTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTtat                                  <  1:908935/140‑1 (MQ=255)                                                                                                     aTTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTtat                                  <  2:935059/140‑1 (MQ=255)                                                                                                     aTTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTtat                                  <  2:615924/140‑1 (MQ=255)                                                                                                      ttCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTtata                                 <  2:329266/140‑1 (MQ=255)                                                                                                          cAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGa                             <  2:261180/140‑1 (MQ=255)                                                                                                              aGTTCTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAg                         <  1:444968/140‑1 (MQ=255)                                                                                                                  cTGAAAAAGGGTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCa                     <  1:60179/140‑1 (MQ=255)                                                                                                                            gTCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACt           >  1:874879/1‑140 (MQ=255)                                                                                                                             tCACTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTg          >  2:653436/1‑140 (MQ=255)                                                                                                                                cTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAg       <  2:838591/140‑1 (MQ=255)                                                                                                                                cTTCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAg       <  1:281568/140‑1 (MQ=255)                                                                                                                                 ttCGGTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGt      >  1:977254/1‑140 (MQ=255)                                                                                                                                     gTGGCCC‑TTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGgc  >  1:348342/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                      AAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGGC  >  NC_000913/209480‑209752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑