Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,061,966	Δ1 bp	coding (1117/2145 nt)	scpA →	methylmalonyl‑CoA mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,061,964	0	C	.	100.0%	375.7 / NA	89	coding (1115/2145 nt)	scpA	methylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (45/44);  total (45/44)

ACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCG  >  NC_000913/3061825‑3062098                                                                                                                                            |                                                                                                                                       actgaACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGc                                                                                                                                        >  1:809248/1‑140 (MQ=255) actgaACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGc                                                                                                                                        >  1:343018/1‑140 (MQ=255)     aaCAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGccgca                                                                                                                                    <  2:809248/140‑4 (MQ=255)     aaCAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGccgca                                                                                                                                    <  2:343018/140‑4 (MQ=255)      aCAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGccgcaa                                                                                                                                   <  2:474551/140‑5 (MQ=255)        aGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAaca                                                                                                                                <  2:190216/140‑1 (MQ=255)           aTCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACAccc                                                                                                                             >  2:69396/1‑140 (MQ=255)               gTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACAcc                                                                                                                              >  2:963382/1‑135 (MQ=255)               gTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACAcc                                                                                                                              <  1:963382/135‑1 (MQ=255)               gTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGat                                                                                                                         >  2:98450/1‑140 (MQ=255)               gTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGat                                                                                                                         >  2:22060/1‑140 (MQ=255)                  taACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGatcat                                                                                                                      <  2:99617/140‑1 (MQ=255)                  taACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGatcat                                                                                                                      >  2:128177/1‑140 (MQ=255)                  taACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGatcat                                                                                                                      >  2:384783/1‑140 (MQ=255)                     caacGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGatcatcat                                                                                                                   >  1:633129/1‑140 (MQ=255)                     caacGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGatcatcat                                                                                                                   >  1:468202/1‑140 (MQ=255)                        cGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGatcat                                                                                                                      <  2:1078389/134‑1 (MQ=255)                        cGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGatcat                                                                                                                      >  1:1078389/1‑134 (MQ=255)                        cGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCa                                                                                                                >  1:462186/1‑140 (MQ=255)                        cGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCa                                                                                                                >  1:889423/1‑140 (MQ=255)                        cGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCa                                                                                                                >  2:836533/1‑140 (MQ=255)                          ttATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAgg                                                                                                              >  2:241812/1‑140 (MQ=255)                          ttATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAgg                                                                                                              >  1:445606/1‑140 (MQ=255)                          ttATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGCCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAgg                                                                                                              >  2:901579/1‑140 (MQ=255)                           tATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGga                                                                                                             <  1:230428/140‑1 (MQ=255)                             tCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATcctgtctc                                                                                                           >  1:31075/1‑134 (MQ=255)                             tCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATcctgtctc                                                                                                           >  1:363115/1‑134 (MQ=255)                                     accaTTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGaa                                                                                                   <  2:889423/140‑1 (MQ=255)                                       caTTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGaa                                                                                                   <  2:763020/138‑1 (MQ=255)                                       caTTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGaa                                                                                                   >  1:763020/1‑138 (MQ=255)                                       caTTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAAct                                                                                                 >  1:502074/1‑140 (MQ=255)                                         ttGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAActct                                                                                               >  2:763665/1‑140 (MQ=255)                                             aGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCg                                                                                           <  1:836533/140‑1 (MQ=255)                                                gctggctgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCAc                                                                                        <  1:98450/140‑1 (MQ=255)                                                gctggctgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCAc                                                                                        <  1:22060/140‑1 (MQ=255)                                                 ctggctgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCAcc                                                                                       >  2:497642/1‑140 (MQ=255)                                                 ctggctgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCAcc                                                                                       >  2:789858/1‑140 (MQ=255)                                                    gctgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTc                                                                                    <  1:497642/140‑1 (MQ=255)                                                     ctgCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCg                                                                                   <  1:763665/140‑1 (MQ=255)                                                     ctgCGACGCTGGGCGGTACTCAGTCAATGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCg                                                                                   <  2:461361/140‑1 (MQ=255)                                                        cGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATc                                                                                <  2:468202/140‑1 (MQ=255)                                                          aCGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCa                                                                              <  2:462186/140‑1 (MQ=255)                                                           cgttGGGCGGTACTCAGTCACTGCATACCACCGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCAc                                                                             <  2:704105/137‑1 (MQ=255)                                                              tGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCa                                                                              >  2:1004752/1‑136 (MQ=255)                                                              tGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCa                                                                              <  1:1004752/136‑1 (MQ=255)                                                              tGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTgg                                                                          <  1:789858/140‑1 (MQ=255)                                                              tGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTgg                                                                          >  2:512187/1‑140 (MQ=255)                                                                ggCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCa                                                                              >  1:513572/1‑134 (MQ=255)                                                                ggCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCa                                                                              <  2:513572/134‑1 (MQ=255)                                                                 gCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCg                                                                       <  1:384783/140‑1 (MQ=255)                                                                 gCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCg                                                                       <  2:502074/140‑1 (MQ=255)                                                                 gCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCg                                                                       <  1:723025/140‑1 (MQ=255)                                                                 gCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCg                                                                       <  1:585216/140‑1 (MQ=255)                                                                   ggTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGa                                                                     <  1:128177/140‑1 (MQ=255)                                                                   ggTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGa                                                                     <  2:460537/140‑1 (MQ=255)                                                                        tCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTa                                                                <  2:1085465/140‑1 (MQ=255)                                                                        tCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTa                                                                <  1:861948/140‑1 (MQ=255)                                                                          aGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCt                                                                 >  2:575777/1‑137 (MQ=255)                                                                          aGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCt                                                                 <  1:575777/137‑1 (MQ=255)                                                                          aGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTAtt                                                              >  2:286612/1‑140 (MQ=255)                                                                            tCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGa                                                                     <  1:503161/131‑1 (MQ=255)                                                                            tCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGa                                                                     >  2:503161/1‑131 (MQ=255)                                                                               cTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCt                                                                 <  2:474620/132‑1 (MQ=255)                                                                               cTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCt                                                                 >  1:474620/1‑132 (MQ=255)                                                                               cTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACAtt                                                         >  1:672496/1‑140 (MQ=255)                                                                                 gCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGa                                                       >  2:114408/1‑140 (MQ=255)                                                                                     aCCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCg                                                   >  1:347098/1‑140 (MQ=255)                                                                                          cGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGAc                                              <  2:672496/140‑1 (MQ=255)                                                                                            ccTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCg                                                   <  1:634190/133‑1 (MQ=255)                                                                                            ccTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCg                                                   >  2:634190/1‑133 (MQ=255)                                                                                            ccTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCg                                            >  2:269634/1‑140 (MQ=255)                                                                                                 gACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCaa                                       <  1:114408/140‑1 (MQ=255)                                                                                                      aGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGt                                  >  2:388667/1‑140 (MQ=255)                                                                                                       gcgcTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTc                                 >  2:937657/1‑140 (MQ=255)                                                                                                       gcgcTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTc                                 >  2:263403/1‑140 (MQ=255)                                                                                                          cTTGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCaaa                              <  1:69396/140‑1 (MQ=255)                                                                                                           ttGGTTTGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAAc                             <  1:937657/140‑1 (MQ=255)                                                                                                               tttGCCTACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGc                         <  1:286612/140‑1 (MQ=255)                                                                                                                     tACCGATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGc                   >  1:197179/1‑140 (MQ=255)                                                                                                                         gATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGc                         >  1:1108936/1‑130 (MQ=255)                                                                                                                         gATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGc                         <  2:1108936/130‑1 (MQ=255)                                                                                                                         gATTTCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCtatt               <  2:347098/140‑1 (MQ=255)                                                                                                                            ttCTCAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATc            <  2:225442/140‑1 (MQ=255)                                                                                                                             tctcAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATcc           <  1:292984/140‑1 (MQ=255)                                                                                                                             tctcAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATcc           >  1:510042/1‑140 (MQ=255)                                                                                                                              ctcAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGc                   >  1:155073/1‑131 (MQ=255)                                                                                                                              ctcAGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGc                   <  2:155073/131‑1 (MQ=255)                                                                                                                                 aGCACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACa       >  2:752304/1‑140 (MQ=255)                                                                                                                                   cACGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGa     >  2:676591/1‑140 (MQ=255)                                                                                                                                     cGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATcc           <  2:765659/132‑1 (MQ=255)                                                                                                                                     cGCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATcc           >  1:765659/1‑132 (MQ=255)                                                                                                                                      gCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCta                 <  2:739778/125‑1 (MQ=255)                                                                                                                                      gCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCta                 >  1:739778/1‑125 (MQ=255)                                                                                                                                      gCATTG‑CCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATcg  <  2:1148115/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                       ACTGAACAGGATCCGTATAACAACGTTATCCGCACCACCATTGAAGCGCTGGCTGCGACGCTGGGCGGTACTCAGTCACTGCATACCAACGCCTTTGACGAAGCGCTTGGTTTGCCTACCGATTTCTCAGCACGCATTGCCCGCAACACCCAGATCATCATCCAGGAAGAATCAGAACTCTGCCGCACCGTCGATCCACTGGCCGGATCCTATTACATTGAGTCGCTGACCGATCAAATCGTCAAACAAGCCAGAGCTATTATCCAACAGATCG  >  NC_000913/3061825‑3062098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑