Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,333,582	(A)8→7	intergenic (‑131/‑128)	rplU ← / → ispB	50S ribosomal subunit protein L21/all‑trans‑octaprenyl‑diphosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,333,575	0	A	.	100.0%	281.0 / NA	67	intergenic (‑124/‑135)	rplU/ispB	50S ribosomal subunit protein L21/all‑trans‑octaprenyl‑diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (33/34);  total (33/34)

AAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTT  >  NC_000913/3333438‑3333706                                                                                                                                           |                                                                                                                                    aaaaCCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaa                                                                                                                                   <  2:131858/140‑1 (MQ=255) aaaaCCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaa                                                                                                                                   <  2:648779/140‑1 (MQ=255)           aCATATAAAACTCCGCTTCCGCGCACCCCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAAc                                                                                                                        <  2:134039/140‑1 (MQ=255)                taAAACTCCGCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTa                                                                                                                   <  2:826942/140‑1 (MQ=255)                taAAACTCCGCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTa                                                                                                                   <  2:563299/140‑1 (MQ=255)                 aaaaCTCCGCTTCCGCGCACACCTTTTCCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATATACGAAG‑AAAAAAACACAACTTGTa                                                                                                                   <  2:543385/140‑1 (MQ=255)                   aaCTCCGCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACACACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaaaaaa                                                                                                                               >  1:477551/1‑126 (MQ=255)                   aaCTCCGCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACACACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaaaaaa                                                                                                                               <  2:477551/126‑1 (MQ=255)                       ccGCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTg                                                                                                                     <  2:120960/131‑1 (MQ=255)                       ccGCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTg                                                                                                                     >  1:120960/1‑131 (MQ=255)                       ccGCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAAc                                                                                                            >  1:270577/1‑140 (MQ=255)                       ccGCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAAc                                                                                                            >  2:589512/1‑140 (MQ=255)                        cGCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACg                                                                                                           >  2:900501/1‑140 (MQ=255)                         gCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGt                                                                                                          >  1:25507/1‑140 (MQ=255)                         gCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGt                                                                                                          >  2:539687/1‑140 (MQ=255)                         gCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGt                                                                                                          >  1:389816/1‑140 (MQ=255)                          cTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGtt                                                                                                         >  2:196400/1‑140 (MQ=255)                          cTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGtt                                                                                                         >  2:832245/1‑140 (MQ=255)                            tCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTa                                                                                                       <  2:25507/140‑1 (MQ=255)                                       cTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCAtt                                                                                            >  1:392706/1‑140 (MQ=255)                                          tt‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCAttttt                                                                                         <  1:1148085/140‑1 (MQ=255)                                             cAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTc                                                                                       <  1:1069185/140‑1 (MQ=255)                                               aTGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAg                                                                                     <  1:196400/140‑1 (MQ=255)                                               aTGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAg                                                                                     <  2:284418/140‑1 (MQ=255)                                               aTGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAg                                                                                     <  1:564345/140‑1 (MQ=255)                                               aTGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAg                                                                                     <  1:832245/140‑1 (MQ=255)                                                tGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGt                                                                                    >  2:99784/1‑140 (MQ=255)                                                  aTTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTAc                                                                                  >  1:131807/1‑140 (MQ=255)                                                   ttCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACa                                                                                 <  2:392706/140‑1 (MQ=255)                                                    tCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACaa                                                                                <  2:529639/140‑1 (MQ=255)                                                    tCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACaa                                                                                <  2:389816/140‑1 (MQ=255)                                                     cAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAAt                                                                               <  2:270577/140‑1 (MQ=255)                                                       gagTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCa                                                                             <  1:718245/140‑1 (MQ=255)                                                         gTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAcc                                                                           <  1:876253/140‑1 (MQ=255)                                                            cgcTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCta                                                                        >  1:78974/1‑140 (MQ=255)                                                              cTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCtata                                                                      <  1:99784/140‑1 (MQ=255)                                                                   aaTATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCt                                                                 <  2:131807/140‑1 (MQ=255)                                                                       ttCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAAcc                                                             >  2:406920/1‑140 (MQ=255)                                                                         cacaATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCAt                                                           >  1:763389/1‑140 (MQ=255)                                                                         cacaATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCAt                                                           >  2:207155/1‑140 (MQ=255)                                                                          acaATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATa                                                          <  1:406920/140‑1 (MQ=255)                                                                           caATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCta                                                                        <  1:754268/125‑1 (MQ=255)                                                                           caATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCta                                                                        >  2:754268/1‑125 (MQ=255)                                                                           caATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCAt                                                           <  1:816688/138‑1 (MQ=255)                                                                           caATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCAt                                                           >  2:816688/1‑138 (MQ=255)                                                                           caATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATaa                                                         <  1:43181/140‑1 (MQ=255)                                                                           caATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATaa                                                         >  2:43181/1‑140 (MQ=255)                                                                             aTAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAAc                                                       <  1:589512/140‑1 (MQ=255)                                                                                 ggCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTa                                                   >  1:156962/1‑140 (MQ=255)                                                                                 ggCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTa                                                   <  1:900501/140‑1 (MQ=255)                                                                                   cgcgAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAg                                                 >  2:480330/1‑140 (MQ=255)                                                                                   cgcgAATATTACGCAAAACGCACGCCTTTGACAAGAGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAg                                                 >  2:77369/1‑140 (MQ=255)                                                                                    gcgAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGt                                                >  2:516113/1‑140 (MQ=255)                                                                                      gAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTg                                              >  2:402422/1‑140 (MQ=255)                                                                                      gAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTg                                              >  2:303215/1‑140 (MQ=255)                                                                                         tatTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCt                                           <  1:516113/140‑1 (MQ=255)                                                                                             aCGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGt                                       <  1:791800/140‑1 (MQ=255)                                                                                              cGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGtt                                      <  1:539687/140‑1 (MQ=255)                                                                                              cGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGtt                                      <  2:763389/140‑1 (MQ=255)                                                                                                 aaaaCGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTcac                                   <  1:308126/140‑1 (MQ=255)                                                                                                  aaaCGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTcaca                                  >  1:287706/1‑140 (MQ=255)                                                                                                  aaaCGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTcaca                                  <  1:207155/140‑1 (MQ=255)                                                                                                  aaaCGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTcaca                                  <  1:402422/140‑1 (MQ=255)                                                                                                     cgaacgcCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGtt                                      <  1:834064/130‑1 (MQ=255)                                                                                                     cgaacgcCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGtt                                      >  2:834064/4‑133 (MQ=255)                                                                                                             tttGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATc                       >  1:222077/1‑140 (MQ=255)                                                                                                                               cAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCt     >  2:1100228/1‑140 (MQ=255)                                                                                                                                aaTACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCtt    >  2:783367/1‑140 (MQ=255)                                                                                                                                aaTACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCtt    >  2:879112/1‑140 (MQ=255)                                                                                                                                aaTACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCtt    >  2:533648/1‑140 (MQ=255)                                                                                                                                  tACACGAAG‑AAAAAAACACAACTTGTACGGTATCGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCtttt  >  2:1101363/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                    AAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTT‑CAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGCTTTT  >  NC_000913/3333438‑3333706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑