Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,094	(G)8→7	noncoding (72/87 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,087	0	G	.	100.0%	192.1 / NA	47	noncoding (79/87 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (26/21);  total (26/21)

CAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAA‑CCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTT  >  NC_000913/4605950‑4606219                                                                                                                                          |                                                                                                                                      caAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggg                                                                                                                                     >  2:788645/1‑140 (MQ=255)  aaaCATTTGCGGCATTACATTTTGCTCCCATCGTGCCTGGAACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAACAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgggg                                                                                                                                    <  2:1016926/140‑1 (MQ=255)  aaaCAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgggg                                                                                                                                    <  1:788645/140‑1 (MQ=255)   aaCAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggggg                                                                                                                                   >  2:665653/1‑140 (MQ=255)      aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggggggga                                                                                                                                >  1:802980/1‑139 (MQ=255)      aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggggggga                                                                                                                                >  1:115236/1‑139 (MQ=255)       gTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgggggggac                                                                                                                               <  1:551558/140‑3 (MQ=255)        ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgggggggact                                                                                                                              >  1:1130684/1‑137 (MQ=255)        ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgggggg                                                                                                                                  <  1:552474/136‑1 (MQ=255)        ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgggggg                                                                                                                                  >  2:552474/1‑136 (MQ=255)          gCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTg                                                                                                                           >  2:39042/1‑140 (MQ=255)          gCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTg                                                                                                                           >  1:126604/1‑140 (MQ=255)                     ttttGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAA‑ccccccacg                                                                                                               <  1:39042/140‑5 (MQ=255)                          cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGt                                                                                                           >  2:192699/1‑140 (MQ=255)                          cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGt                                                                                                           <  2:126604/140‑1 (MQ=255)                          cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGt                                                                                                           >  2:1039184/1‑140 (MQ=255)                                  gTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGacac                                                                                                   >  2:668802/1‑140 (MQ=255)                                     ccTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATCGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTaa                                                                                                <  1:1083473/140‑1 (MQ=39)                                          cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCt                                                                                           >  2:22133/1‑140 (MQ=255)                                                 tGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                                                                    <  1:511316/140‑1 (MQ=255)                                                     ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                                                                >  2:923458/1‑140 (MQ=255)                                                     ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                                                                >  1:92935/1‑140 (MQ=255)                                                      gCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                               <  2:92935/140‑1 (MQ=255)                                                      gCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                               >  2:236974/1‑140 (MQ=255)                                                       catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGG‑AGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                                                             >  2:837294/1‑140 (MQ=37)                                                                   gatAATGAATTTGAATCAGGGATGAAAAATCAAGG‑AGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                 <  1:837294/140‑1 (MQ=25)                                                                       aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                                                              >  1:149237/1‑140 (MQ=255)                                                                        tGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                             >  2:902553/1‑140 (MQ=255)                                                                        tGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                             >  2:864669/1‑140 (MQ=255)                                                                          aaTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                           <  1:902553/140‑1 (MQ=255)                                                                           aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                          >  2:835905/1‑140 (MQ=255)                                                                           aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                          >  1:1142165/1‑140 (MQ=255)                                                                               gAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac                                                      <  2:1142165/140‑1 (MQ=255)                                                                                    aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCa                                                 >  1:589715/1‑140 (MQ=255)                                                                                    aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCa                                                 >  2:1139931/1‑140 (MQ=255)                                                                                      ggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATc                                               >  2:133232/1‑140 (MQ=255)                                                                                         tGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTa                                            <  1:133232/140‑1 (MQ=255)                                                                                          gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAc                                           >  2:403317/1‑140 (MQ=255)                                                                                                    ggAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGat                                 <  1:1139931/140‑1 (MQ=255)                                                                                                     gaagaaACAAGAAATGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGata                                <  2:802980/140‑1 (MQ=21)                                                                                                           aCAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCt                          <  1:923458/140‑1 (MQ=255)                                                                                                             aaGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCg                        <  2:823824/140‑1 (MQ=255)                                                                                                               gAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGtt                      <  2:149237/140‑1 (MQ=255)                                                                                                               gAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGtt                      <  2:127533/140‑1 (MQ=255)                                                                                                               gAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGtt                      <  1:835905/140‑1 (MQ=255)                                                                                                                 aaGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATc                              <  1:214728/130‑1 (MQ=255)                                                                                                                 aaGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATc                              >  2:214728/1‑130 (MQ=255)                                                                                                                   ggAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGt                  >  1:1042163/1‑140 (MQ=255)                                                                                                                    gAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTg                 >  1:314576/1‑140 (MQ=255)                                                                                                                      aGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCg               <  2:314576/140‑1 (MQ=255)                                                                                                                      aGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCg               <  1:864669/140‑1 (MQ=255)                                                                                                                           aGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg          >  1:450575/1‑140 (MQ=255)                                                                                                                            gATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggg         <  1:403317/140‑1 (MQ=255)                                                                                                                               aaTTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggggg      >  1:612897/1‑140 (MQ=255)                                                                                                                                 ttGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGAc    >  1:907271/1‑140 (MQ=255)                                                                                                                                  tGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACt   >  1:21497/1‑140 (MQ=255)                                                                                                                                   ggTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACtt  <  2:21497/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      CAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAA‑CCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTT  >  NC_000913/4605950‑4606219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑