Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	727,514	(T)7→6	coding (1219/1674 nt)	kdpA ←	K(+) transporting P‑type ATPase subunit KdpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	727,508	0	T	.	100.0%	182.5 / NA	44	coding (1225/1674 nt)	kdpA	K(+) transporting P‑type ATPase subunit KdpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (23/21);  total (23/21)

CGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATC  >  NC_000913/727370‑727638                                                                                                                                           |                                                                                                                                   cGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGAtt                                                                                                                                   >  1:1008527/1‑140 (MQ=255) cGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGAtt                                                                                                                                   >  2:824411/1‑140 (MQ=255)    tAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGAttttt                                                                                                                                >  1:113689/1‑140 (MQ=255)      aaaCCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGAtttttta                                                                                                                              <  1:610399/140‑2 (MQ=255)       aaCCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGAttttttac                                                                                                                             <  2:444503/140‑3 (MQ=255)         ccATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGAttttttaccc                                                                                                                           <  1:1061956/140‑5 (MQ=255)           aTGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAg                                                                                                                        >  2:518713/1‑140 (MQ=255)             gCGGGTCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGAtt                                                                                                                                   <  2:1022092/128‑1 (MQ=255)             gCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGAtt                                                                                                                                   >  1:1022092/1‑128 (MQ=255)              cGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGata                                                                                                                     <  2:763127/140‑1 (MQ=255)                 gCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTc                                                                                                                  <  1:824411/140‑1 (MQ=255)                  ccAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTcc                                                                                                                 >  1:801092/1‑140 (MQ=255)                    aGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCgg                                                                                                               <  1:1147517/140‑1 (MQ=255)                      ggTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGgtg                                                                                                             <  1:518713/140‑1 (MQ=255)                         tGAGCATGGCTCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTAc                                                                                                          <  2:707949/140‑1 (MQ=255)                           aGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGa                                                                                                        >  2:643648/1‑140 (MQ=255)                           aGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGa                                                                                                        >  1:894034/1‑140 (MQ=255)                            gCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGAc                                                                                                       <  2:801092/140‑1 (MQ=255)                                ggCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAat                                                                                                   >  1:18829/1‑140 (MQ=255)                                ggCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGGTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGCGTACGACCaac                                                                                                   >  2:113696/1‑139 (MQ=255)                                  cgcTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAatca                                                                                                 >  1:261346/1‑140 (MQ=255)                                   gcTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAatcat                                                                                                <  1:992410/140‑1 (MQ=255)                                        gTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGcc                                                                                           <  1:643648/140‑1 (MQ=255)                                        gTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGcc                                                                                           <  2:290298/140‑1 (MQ=255)                                            ggCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGc                                                                                       >  1:661596/1‑140 (MQ=255)                                                 cGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTCCCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATaa                                                                                  <  2:113689/140‑1 (MQ=255)                                                  ggTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATaaa                                                                                 >  2:316169/1‑140 (MQ=255)                                                   gTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAac                                                                                >  1:88899/1‑140 (MQ=255)                                                                 cgccgcGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGAc                                                                  <  2:642491/140‑1 (MQ=255)                                                                  gccgcGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACa                                                                 <  2:1008527/140‑1 (MQ=255)                                                                      cgcCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACATCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAgg                                                                    >  2:1081675/1‑133 (MQ=255)                                                                      cgcCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACATCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAgg                                                                    <  1:1081675/133‑1 (MQ=255)                                                                       gcCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAg                                                            <  2:261346/140‑1 (MQ=255)                                                                         ccATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCACCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGca                                                          <  2:18829/140‑1 (MQ=255)                                                                            tCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGcatca                                                       <  1:316169/140‑1 (MQ=255)                                                                              aGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATg                                                     <  2:88899/140‑1 (MQ=255)                                                                                     gCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATg                                                     <  2:46209/133‑1 (MQ=255)                                                                                     gCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATg                                                     >  1:46209/1‑133 (MQ=255)                                                                                      cGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAg                                             >  1:435612/1‑140 (MQ=255)                                                                                        tgggggTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTCCCCAAATATCCCGGTGTCCGACAAATCATCAGCCCGGAAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGAc                                           <  2:669770/138‑1 (MQ=255)                                                                                                      aTTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACAccg                             >  2:590219/1‑140 (MQ=255)                                                                                                       ttGCCAGTGCAGTCAGTTTCATCTCGCGTCCGTCGA‑TTTTTTCCCCAGATATCCCGGTGTCCGACCAATCCTCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACAccgc                            <  2:254365/140‑1 (MQ=255)                                                                                                          ccAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGa                         >  2:588556/1‑140 (MQ=255)                                                                                                          ccAGTGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGa                         >  2:585585/1‑140 (MQ=255)                                                                                                              tGCAGTCAGTTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAacac                     <  2:894034/140‑1 (MQ=255)                                                                                                                cagtcagtTTCATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAacctgt                   >  1:662613/1‑136 (MQ=255)                                                                                                                          cATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAAt         >  2:697538/1‑140 (MQ=255)                                                                                                                          cATCTCGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAAt         >  2:41198/1‑140 (MQ=255)                                                                                                                            tctcGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCGTGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAAttt       >  2:451741/1‑140 (MQ=255)                                                                                                                             ctcGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACt                 >  2:610863/1‑129 (MQ=255)                                                                                                                             ctcGCGTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACt                 <  1:610863/129‑1 (MQ=255)                                                                                                                               cgcgTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCa    <  2:661596/140‑1 (MQ=255)                                                                                                                               cgcgTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCa    >  2:970352/1‑140 (MQ=255)                                                                                                                                 cgTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATc  >  2:906976/1‑140 (MQ=255)                                                                                                                                 cgTACGTCGA‑TTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATc  >  2:908491/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                   CGCTAAAACCATGCGGGCCAGGGTTGAGCATGGCGCTACGTCCGGCGTCGGTCATCATCGCCAACGCCGCGCCCATCAGCACCAGCGTCGGGGTAACCAGAATTGCCAGTGCAGTCAGTTTCATCTCGCGTACGTCGATTTTTTTACCCAGATATTCCGGTGTACGACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACCGCCGAACACCACTTCACCAATTTGCATC  >  NC_000913/727370‑727638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑