| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,223,984 | (A)7→6 | intergenic (+77/+295) | ycgI → / ← minE | protein YcgI/Z‑ring positioning protein MinE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,223,978 | 0 | A | . | 97.9% | 189.5 / ‑2.1 | 48 | intergenic (+71/+301) | ycgI/minE | protein YcgI/Z‑ring positioning protein MinE |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (19/28); total (19/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑ACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACAT > NC_000913/1223841‑1224109 | gtATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCaaa > 1:998666/1‑140 (MQ=255) tataGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCaaaa < 2:998666/140‑1 (MQ=255) aGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCaaaaaag < 2:893647/140‑2 (MQ=255) gCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCaaaaaagc > 1:620231/1‑138 (MQ=255) aTGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCg > 1:59477/1‑140 (MQ=255) ggTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGcc > 1:1046727/1‑140 (MQ=255) ggTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGcc > 2:930463/1‑140 (MQ=255) gTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCa > 2:836447/1‑140 (MQ=255) gTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCa > 1:73179/1‑140 (MQ=255) gCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGt > 2:283011/1‑140 (MQ=255) ccGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGtt > 2:644136/1‑140 (MQ=255) ccGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGtt > 2:1002186/1‑140 (MQ=255) ggTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTAc > 2:878289/1‑140 (MQ=255) gAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCggg < 1:878289/140‑1 (MQ=255) aaTCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑GAAAAAGCCCGCCAGTTACGGCGGGa < 1:149980/140‑1 (MQ=255) tCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGaaa < 2:833191/140‑1 (MQ=255) ccGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTGGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCt < 1:283011/140‑1 (MQ=255) ccGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTa < 1:39443/127‑1 (MQ=255) ccGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTa > 2:39443/1‑127 (MQ=255) ccGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCt < 1:1002186/140‑1 (MQ=255) ccGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCt < 2:73179/140‑1 (MQ=255) ccGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCt < 2:478333/140‑1 (MQ=255) gTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCa < 1:930463/140‑1 (MQ=255) ggAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATc < 1:830701/140‑1 (MQ=255) ggAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATc < 2:620231/140‑1 (MQ=255) ggAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATc < 2:59477/140‑1 (MQ=255) cgGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAAAcccatcccattg > 2:965628/1‑138 (MQ=39) gTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGga < 2:487455/140‑1 (MQ=255) gTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGga < 2:105389/140‑1 (MQ=255) gTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTAGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGga < 2:654355/140‑1 (MQ=255) agacagggTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTGCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGG‑AAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGga < 1:627773/134‑1 (MQ=255) tgAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGaa < 2:1046727/140‑1 (MQ=255) gTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAAACCCATCCTATTGGGGA‑GGAAAGAATAAAGaa > 2:356272/1‑140 (MQ=255) gACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCggg < 2:675158/140‑1 (MQ=255) tCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTat < 1:59341/140‑1 (MQ=255) tCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAAACTCATCCTATTGGGGA‑GCAAAGAAAAAAGAAAATTCGGGGGTat > 2:771749/1‑140 (MQ=255) aaCGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAAACCCATCCTATGGGAGA‑GCAAAGAATAATGAAAATGCGGGGGTatca > 2:450148/1‑140 (MQ=255) cGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTatcatc < 1:722172/140‑1 (MQ=255) gATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCAtct < 2:322716/140‑1 (MQ=255) aTTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCAtctc < 2:177543/140‑1 (MQ=255) aTTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCAtctc < 1:274004/140‑1 (MQ=255) acaggcgcTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAg < 1:267800/136‑1 (MQ=255) aaCTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAg < 1:1095941/140‑1 (MQ=255) aaCTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAg < 2:654639/140‑1 (MQ=255) aCTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCACCTCCCAGt < 1:1114010/140‑1 (MQ=255) gcgcTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGtata < 1:751325/140‑1 (MQ=255) cAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAAACTCCTCCTATTGGGGA‑GGAAAGAAAAATGAAAATGCGGGGGTATTATCTCCCAGTATATTCATAcccgt > 2:267800/1‑136 (MQ=255) ttGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGGAAACTCCTCCTATGGGAGG‑GGAATGAATAATGAAATTGCGGGGGTATTATCTCCCCGTATATCCATACTAAAAAAAAAGGTATTTTTTCAAc > 2:788599/1‑140 (MQ=255) ttGAAAGGGAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCCCCTCCCATGGGGGA‑GCAAAGAAAAAAGAAAATGCGGGGGTTTCATCTCCCCGCATATCCCTACTAACAATAAAGTTATTTTATCAAc > 2:716755/1‑140 (MQ=255) gAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAAACTCATCCTTTTGGGGA‑GGAAAGAAAAATAAAAATTCGGGGGTATCATCTCCCCCTATATTCATACTAAAAAAAAAGATATTTATTTAAACAAGCATa > 2:977967/1‑140 (MQ=255) gAAAATTCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAAAAACCTTCTATTGGGGAGGAAAGGAAAAAAAAAATTG‑GGGGGTATGATCTCCCCATATATTCCAACTTAAAAAAAAATTTTTTTTTCAACCAGGCCTa > 2:96163/1‑140 (MQ=255) ttCTGGGGC‑AAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑GCAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACAt < 1:931023/140‑1 (MQ=255) | GTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGA‑ACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACAT > NC_000913/1223841‑1224109 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |