| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,243,818 | (T)5→4 | coding (710/735 nt) | ycgR ← | flagellar brake protein YcgR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,243,814 | 0 | T | . | 98.2% | 214.9 / ‑1.8 | 54 | coding (714/735 nt) | ycgR | flagellar brake protein YcgR |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (27/26); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.00e-01 | |||||||||||
CAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGCTTTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACGGGGAGTGGTGATGGTTTCATTCTTGCCATCAA > NC_000913/1243677‑1243938 | cAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGCttt > 1:638466/1‑140 (MQ=255) aaaTCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGCttttc < 1:81903/140‑2 (MQ=255) aaTCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGCttttcc < 2:219554/140‑3 (MQ=255) tCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGCTTTTcccg < 2:207270/140‑2 (MQ=255) tGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTctcg > 1:110470/1‑140 (MQ=255) tGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATATACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTctcg < 1:792421/140‑1 (MQ=255) tGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTctcgctcg < 2:638466/140‑1 (MQ=255) gACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCgagag > 1:1028468/1‑140 (MQ=255) aCGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCgagaga < 2:126526/140‑1 (MQ=255) aCGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCgagaga > 1:945214/1‑140 (MQ=255) cTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAaataa < 2:248531/140‑1 (MQ=255) cGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATc < 1:495191/140‑1 (MQ=255) aaCACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCg < 1:168081/140‑1 (MQ=255) aaCACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCg < 1:417845/140‑1 (MQ=255) acGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTg > 2:244787/1‑140 (MQ=255) cGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGt < 1:244787/140‑1 (MQ=255) tAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTaa > 2:325861/1‑140 (MQ=255) tAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTaa > 1:608402/1‑140 (MQ=255) gcgAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGc < 1:919109/140‑1 (MQ=255) gcgAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGc < 2:971122/140‑1 (MQ=255) cgAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTa > 1:1052679/1‑134 (MQ=255) cgAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTa < 2:1052679/134‑1 (MQ=255) cgAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGcc > 2:165055/1‑140 (MQ=255) gccgcAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCggg < 1:165055/140‑1 (MQ=255) cAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCa < 1:861694/128‑1 (MQ=255) cAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCa > 2:861694/1‑128 (MQ=255) cAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGa > 2:461560/1‑140 (MQ=255) aGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGAc > 2:508784/1‑140 (MQ=255) aGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGAc < 1:461560/140‑1 (MQ=255) cTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGtt < 1:508784/140‑1 (MQ=255) atatCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACg > 1:125586/1‑140 (MQ=255) atatCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACg > 2:160158/1‑140 (MQ=255) atCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGa < 2:542968/140‑1 (MQ=255) aaCTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGATGTTAAGAAAACGGAAGCTCAg < 2:608402/140‑1 (MQ=255) aaCTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAg > 2:530937/1‑140 (MQ=255) cTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAGTCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGAc > 2:873408/1‑140 (MQ=255) cTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCt > 2:1075327/1‑135 (MQ=255) cTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCt < 1:1075327/135‑1 (MQ=255) cTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGAc > 2:477749/1‑140 (MQ=255) cTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGAc < 1:530937/140‑1 (MQ=255) cTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGAc > 2:663636/1‑140 (MQ=255) gAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACggg < 1:477749/140‑1 (MQ=255) gAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACggg > 1:911710/1‑140 (MQ=255) gAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACggg < 2:945214/140‑1 (MQ=255) aGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACgggg < 2:911710/140‑1 (MQ=255) cAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACGGGGAg < 1:899198/140‑1 (MQ=255) aCTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACGGGGAgtggtg > 2:1087510/1‑140 (MQ=255) aCTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACGGGGAgtggtg > 2:600161/1‑140 (MQ=255) aCTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACGGGGAgtggtg > 1:605634/1‑140 (MQ=255) cTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAGTCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACGGGGAGTGGTGa < 1:873408/140‑1 (MQ=255) gACGCGATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACGGGGAGTGGTGATgg > 1:1006336/1‑140 (MQ=255) cgcgATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACGGGGAGTGGTGATGGtt > 1:1028989/1‑140 (MQ=255) cgATGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACGGGGAGTGGTGATGGTTTc > 2:811907/1‑140 (MQ=255) aTGTAAATCAGTCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACGGGGAGTGGTGATGGTTTCAt > 2:688164/1‑140 (MQ=255) tCGCGCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACGGGGAGTGGTGATGGTTTCATTCTTGCCatca > 2:1098955/1‑140 (MQ=255) cgcgCACTTTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACGGGGAGTGGTGATGGTTTCATTCTTGCCatcaa < 2:563261/140‑1 (MQ=255) cgcgCACTGTGTCCGC‑TTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACGGGGAGTGGTGATGGTTTCATTCTTGCCatcaa < 1:470325/140‑1 (MQ=255) | CAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTGGACGCGATGTAAATCAGTCGCGCACTTTGTCCGCTTTTTCCCGGGCTTCTCGCTCGAGAGAGAAAATAATCCGCTGTAATTGCCGCTCCACCGTCGGGCTGACGTTAAGAAAACGGAAGCTCAGACGGGGAGTGGTGATGGTTTCATTCTTGCCATCAA > NC_000913/1243677‑1243938 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |