Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	77,593	(T)8→7	noncoding (227/227 nt)	sgrS →	small regulatory RNA SgrS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	77,586	0	T	.	100.0%	319.6 / NA	76	noncoding (220/227 nt)	sgrS	small regulatory RNA SgrS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (38/38);  total (38/38)

AAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACA  >  NC_000913/77450‑77715                                                                                                                                         |                                                                                                                                  aaGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGtttt                                                                                                                                >  2:549279/1‑140 (MQ=255)        ccACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGtttttttattc                                                                                                                         >  2:912112/1‑136 (MQ=255)        ccACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGtttttttattc                                                                                                                         >  2:858919/1‑136 (MQ=255)        ccACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGtttttttattc                                                                                                                         >  1:490581/1‑136 (MQ=255)         cacaACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATtct                                                                                                                       >  1:290274/1‑140 (MQ=255)                aTTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGCATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCcgcg                                                                                                                <  2:795568/140‑1 (MQ=255)                 ttAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGtttttt                                                                                                                              <  2:60290/126‑1 (MQ=255)                 ttAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGtttttt                                                                                                                              >  1:60290/1‑126 (MQ=255)                 ttAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCgcgc                                                                                                               >  1:1049436/1‑140 (MQ=255)                 ttAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCgcgc                                                                                                               >  2:258048/1‑140 (MQ=255)                   aaCCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTa                                                                                                             >  2:567982/1‑140 (MQ=255)                     ccATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTaaa                                                                                                           <  1:549279/140‑1 (MQ=255)                        tGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAg                                                                                                        >  1:1089620/1‑140 (MQ=255)                         gCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTaaa                                                                                                           <  2:98897/136‑1 (MQ=255)                         gCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTaaa                                                                                                           >  1:98897/1‑136 (MQ=255)                          cTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAggg                                                                                                      <  2:748693/140‑1 (MQ=255)                          cTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAggg                                                                                                      >  1:675626/1‑140 (MQ=255)                           ttGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGa                                                                                                     >  1:97784/1‑140 (MQ=255)                               aGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTaaaa                                                                                                          <  1:530420/131‑1 (MQ=255)                               aGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTaaaa                                                                                                          >  2:530420/1‑131 (MQ=255)                                ggACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTa                                                                                                >  1:288251/1‑140 (MQ=255)                                ggACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTa                                                                                                <  2:1089620/140‑1 (MQ=255)                                   cTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAggg                                                                                                      <  2:923244/131‑1 (MQ=255)                                   cTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAggg                                                                                                      >  1:923244/1‑131 (MQ=255)                                   cTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGa                                                                                             >  1:1102658/1‑140 (MQ=255)                                    tGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTTAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGAt                                                                                            >  2:1085967/1‑140 (MQ=255)                                    tGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGAt                                                                                            >  1:164708/1‑140 (MQ=255)                                    tGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGAt                                                                                            <  1:535947/140‑1 (MQ=255)                                    tGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGAt                                                                                            <  1:547151/140‑1 (MQ=255)                                        gCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTgg                                                                                        <  2:75543/140‑1 (MQ=255)                                          aGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGAt                                                                                      <  2:1049436/140‑1 (MQ=255)                                            tGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGt                                                                                                 >  1:479739/1‑127 (MQ=255)                                            tGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGt                                                                                                 <  2:479739/127‑1 (MQ=255)                                            tGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGa                                                                                             <  1:641612/131‑1 (MQ=255)                                            tGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGa                                                                                             >  2:641612/1‑131 (MQ=255)                                              ggATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATg                                                                                  >  2:861723/1‑140 (MQ=255)                                                aTGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATaa                                                                                    <  2:396534/136‑1 (MQ=255)                                                aTGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATaa                                                                                    >  1:396534/1‑136 (MQ=255)                                                aTGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGAc                                                                                >  2:307464/1‑140 (MQ=255)                                                aTGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGAc                                                                                >  2:692869/1‑140 (MQ=255)                                                 tGACCGCATTTCTGAAAGTTGCCTTCCCTGCATCATGTGTGAGTGAGTATTGTTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACg                                                                               <  2:1102658/140‑1 (MQ=255)                                                   aCCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGAt                                                                             >  1:904393/1‑140 (MQ=255)                                                       cAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCt                                                                         <  2:288251/140‑1 (MQ=255)                                                       cAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCt                                                                         <  2:290274/140‑1 (MQ=255)                                                        aaTTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTc                                                                        <  1:912112/140‑1 (MQ=255)                                                          ttCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGc                                                                      <  2:642738/140‑1 (MQ=255)                                                               aaaGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTAt                                                                 >  2:750136/1‑140 (MQ=255)                                                                  gTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGaa                                                              <  2:904393/140‑1 (MQ=255)                                                                  gTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGaa                                                              <  1:861723/140‑1 (MQ=255)                                                                  gTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGaa                                                              <  1:258048/140‑1 (MQ=255)                                                                      aCTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACggg                                                          >  2:362436/1‑139 (MQ=255)                                                                                  catGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCa                                              <  2:164708/140‑1 (MQ=255)                                                                                          aCTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCt                                      >  2:426425/1‑140 (MQ=255)                                                                                            tGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTgg                                    <  1:224972/140‑1 (MQ=255)                                                                                             gcgtgttGGTGTAAATGGTCCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGt                                   <  2:490581/135‑1 (MQ=255)                                                                                             gAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGt                                   <  1:750136/140‑1 (MQ=255)                                                                                              aGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTc                                  >  2:808519/1‑140 (MQ=255)                                                                                              aGTATTGGTGAAAAACCCCCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTc                                  <  1:858919/140‑1 (MQ=255)                                                                                               gTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCg                                 >  1:903635/1‑140 (MQ=255)                                                                                                tATTGGTGTAAAATCCCCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGc                                <  2:434101/140‑1 (MQ=255)                                                                                                tATTGGTGTAAAATCCCCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGc                                <  2:1006658/140‑1 (MQ=255)                                                                                                tATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTAcgcg                                                 <  2:859762/123‑1 (MQ=255)                                                                                                tATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTAcgcg                                                 >  1:859762/1‑123 (MQ=255)                                                                                                tATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGt                                   <  2:96052/137‑1 (MQ=255)                                                                                                tATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGt                                   >  1:96052/1‑137 (MQ=255)                                                                                                tATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCg                                 <  2:318708/139‑1 (MQ=255)                                                                                                tATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCg                                 >  1:318708/1‑139 (MQ=255)                                                                                                tATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGc                                >  1:1006658/1‑140 (MQ=255)                                                                                                     gtgtAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTa                           >  1:261059/1‑140 (MQ=255)                                                                                                        tAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTatga                        <  2:675626/140‑1 (MQ=255)                                                                                                        tAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTatga                        >  1:414675/1‑140 (MQ=255)                                                                                                         aaaaTCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCttt                             >  2:815953/1‑134 (MQ=255)                                                                                                         aaaaTCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCttt                             <  1:815953/134‑1 (MQ=255)                                                                                                          aaaTCACCCGCTAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGt                                   <  1:773831/127‑1 (MQ=255)                                                                                                          aaaTCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGt                                   >  2:773831/1‑127 (MQ=255)                                                                                                          aaaTCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTatgatg                      >  1:990931/1‑140 (MQ=255)                                                                                                          aaaTCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTatgatg                      >  2:703553/1‑140 (MQ=255)                                                                                                               aCCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGt                                   >  1:555219/1‑122 (MQ=255)                                                                                                               aCCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGt                                   <  2:555219/122‑1 (MQ=255)                                                                                                                cccGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTg                >  2:1128671/1‑140 (MQ=255)                                                                                                                  cGCCAGCAGATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGc              <  2:97784/140‑1 (MQ=255)                                                                                                                        cagATTATACCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGgc        <  2:3312/140‑1 (MQ=255)                                                                                                                              ataCCTGCTGG‑TTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACa  <  1:307464/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  AAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACA  >  NC_000913/77450‑77715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑