Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,787,398	(T)5→4	intergenic (‑286/‑47)	ppsA ← / → ppsR	phosphoenolpyruvate synthetase/posphoenolpyruvate synthetase regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,787,394	0	T	.	100.0%	204.4 / NA	49	intergenic (‑282/‑51)	ppsA/ppsR	phosphoenolpyruvate synthetase/posphoenolpyruvate synthetase regulatory protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (23/26);  total (23/26)

CGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAA  >  NC_000913/1787255‑1787526                                                                                                                                            |                                                                                                                                     cgcAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCGt                                                                                                                                      >  2:232680/1‑140 (MQ=255) cgcAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCGt                                                                                                                                      >  1:861517/1‑140 (MQ=255) cgcAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCGt                                                                                                                                      >  1:827981/1‑140 (MQ=255) cgcAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCGt                                                                                                                                      >  2:889523/1‑140 (MQ=255) cgcAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCGt                                                                                                                                      >  2:79542/1‑140 (MQ=255)      aTCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCGttttaa                                                                                                                                 >  2:410062/1‑138 (MQ=255)          gCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTa                                                                                                                            <  1:232680/140‑1 (MQ=255)            tctctTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATg                                                                                                                          <  1:1057331/140‑1 (MQ=255)             ctctTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGc                                                                                                                         <  2:1082868/140‑1 (MQ=255)                 tCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTc                                                                                                                     >  2:539358/1‑140 (MQ=255)                               aTAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCt                                                                                                       <  1:79542/140‑1 (MQ=255)                                tAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTg                                                                                                      <  2:438999/140‑1 (MQ=255)                                         ggTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACggg                                                                                             >  2:63822/1‑140 (MQ=255)                                             taAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGgaaga                                                                                         >  1:115244/1‑140 (MQ=255)                                             taAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGgaaga                                                                                         <  2:115244/140‑1 (MQ=255)                                                  gAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACaaa                                                                                    <  1:539358/140‑1 (MQ=255)                                                     aCCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGgaag                                                                                          <  1:593437/131‑1 (MQ=255)                                                     aCCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGgaag                                                                                          >  2:593437/1‑131 (MQ=255)                                                       cATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGa                                                                               <  1:410062/140‑1 (MQ=255)                                                       cATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGa                                                                               <  2:861517/140‑1 (MQ=255)                                                       cATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGa                                                                               <  1:368548/140‑1 (MQ=255)                                                         tAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATa                                                                             >  2:479413/1‑140 (MQ=255)                                                            aaatgaaatgCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATg                                                                          <  2:763251/140‑1 (MQ=255)                                                             aatgaaatgCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGc                                                                         <  1:1041183/140‑1 (MQ=255)                                                                gaaatgCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGt                                                                      >  2:407170/1‑140 (MQ=255)                                                                 aaatgCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGtt                                                                     >  2:74065/1‑140 (MQ=255)                                                                  aatgCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTg                                                                    >  2:20990/1‑140 (MQ=255)                                                                        gTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGcc                                                              <  1:63822/140‑1 (MQ=255)                                                                        gTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGcc                                                              <  1:479413/140‑1 (MQ=255)                                                                                   aaataaaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCAc                                                            >  1:264464/1‑131 (MQ=255)                                                                                   aaataaaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCAc                                                            <  2:63414/131‑1 (MQ=255)                                                                                   aaataaaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCAc                                                            >  1:63414/1‑131 (MQ=255)                                                                                   aaataaaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCAc                                                            <  2:264464/131‑1 (MQ=255)                                                                                   aaataaaatTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTtat                                                   >  1:963318/1‑140 (MQ=255)                                                                                              aaGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATgg                                        >  2:703686/1‑140 (MQ=255)                                                                                               aGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGt                                       >  1:887185/1‑140 (MQ=255)                                                                                               aGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGt                                       <  1:889523/140‑1 (MQ=255)                                                                                               aGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGt                                       <  1:787361/140‑1 (MQ=255)                                                                                                 gTTCATTTTATAAACCAGACACAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTTTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTAc                                     <  2:1045048/140‑1 (MQ=255)                                                                                                               ccAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCgg                       <  1:703686/140‑1 (MQ=255)                                                                                                               ccAGACATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCgg                       <  1:512886/140‑1 (MQ=255)                                                                                                                   aCATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGt                   >  1:471761/1‑140 (MQ=255)                                                                                                                   aCATAACGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGt                   >  2:96764/1‑140 (MQ=255)                                                                                                                        aCGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCgg                       <  2:613049/131‑1 (MQ=255)                                                                                                                        aCGTTCTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCgg                       >  1:613049/1‑131 (MQ=255)                                                                                                                             cTACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGacac         >  2:370920/1‑140 (MQ=255)                                                                                                                              tACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACg        >  2:183818/1‑140 (MQ=255)                                                                                                                              tACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACg        >  1:953982/1‑140 (MQ=255)                                                                                                                              tACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACg        >  1:850942/1‑140 (MQ=255)                                                                                                                              tACGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACg        >  1:44925/1‑140 (MQ=255)                                                                                                                               aCGCTTCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGc       >  2:250926/1‑140 (MQ=255)                                                                                                                                    tCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTaa  <  2:850942/140‑1 (MQ=255)                                                                                                                                    tCTGTGCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTaa  <  2:953982/140‑1 (MQ=255)                                                                                                                                      tgtgCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGaca          >  2:377340/1‑130 (MQ=255)                                                                                                                                      tgtgCG‑TTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGaca          <  1:377340/130‑1 (MQ=255)                                                                                                                                            |                                                                                                                                     CGCAAATCTGCTCTCTTCGACAATTTCCACATAAACCATCGGTATAAACGAAACCATAAAAATGAAATGCTGTTTTCATAAAAAATAAAATTGAAGGTTCATTTTATAAACCAGACATAACGTTCTACGCTTCTGTGCGTTTTTAATTTATGCTTTCATAGAATTATGTCTGCATCACGGGAAGAACAAAATGGATAATGCTGTTGATCGCCACGTTTTTTATATTTCTGATGGTACGGCAATAACTGCGGAGGTATTAGGACACGCAGTAA  >  NC_000913/1787255‑1787526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑