Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,253,976	(T)5→4	coding (170/1251 nt)	nupX ←	putative nucleoside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,253,972	0	T	.	100.0%	316.0 / NA	75	coding (174/1251 nt)	nupX	putative nucleoside transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (39/36);  total (39/36)

GTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTC  >  NC_000913/2253839‑2254104                                                                                                                                      |                                                                                                                                     gTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGACttttcag                                                                                                                                >  2:398487/1‑137 (MQ=255)         cTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCaa                                                                                                                       >  1:423280/1‑140 (MQ=255)         cTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCaa                                                                                                                       <  2:722917/140‑1 (MQ=255)         cTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCaa                                                                                                                       <  2:611661/140‑1 (MQ=255)             aaCCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACACCAAAAGCGAC‑TTTTCAGCGACCCAACGcc                                                                                                                   <  1:417699/140‑1 (MQ=255)                  aaGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGc                                                                                                              >  2:529574/1‑140 (MQ=255)                   aGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCg                                                                                                             <  1:607927/140‑1 (MQ=255)                     aTAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGt                                                                                                           <  1:686003/140‑1 (MQ=255)                       aaaTCCTGCACCATCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTaa                                                                                                         <  1:109437/140‑1 (MQ=255)                       aaaTCCTGCACCATCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCC‑AAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAAc                                                                                                        <  1:643269/140‑1 (MQ=255)                               cACCATCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAAc                                                                                                        <  1:1043040/133‑1 (MQ=255)                               cACCATCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAAc                                                                                                        >  2:1043040/1‑133 (MQ=255)                                  cATCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATaa                                                                                              <  1:840582/140‑1 (MQ=255)                                   aTCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAAt                                                                                             >  2:488881/1‑140 (MQ=255)                                     cAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATgc                                                                                           >  1:2121/1‑140 (MQ=255)                                     cAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATgc                                                                                           >  1:484734/1‑140 (MQ=255)                                            ggTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAAt                                                                                    >  1:569580/1‑140 (MQ=255)                                              tATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCa                                                                                  <  2:569580/140‑1 (MQ=255)                                               aTCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCAc                                                                                 >  1:941239/1‑140 (MQ=255)                                                tCCATTTTCGGTCCGACCAGAGAATCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCa                                                                                  <  1:804787/138‑1 (MQ=255)                                                tCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCa                                                                                  >  2:804787/1‑138 (MQ=255)                                                tCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACg                                                                                <  2:344805/140‑1 (MQ=255)                                                 ccATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATg                                                                                            >  2:123243/1‑127 (MQ=255)                                                 ccATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATg                                                                                            <  1:123243/127‑1 (MQ=255)                                                  cATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGAc                                                                              >  2:650278/1‑140 (MQ=255)                                                  cATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACCCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGAc                                                                              <  2:1756/140‑1 (MQ=255)                                                      ttCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGt                                                                          >  2:420254/1‑140 (MQ=255)                                                        cGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTaa                                                                        >  1:889934/1‑140 (MQ=255)                                                        cGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTaa                                                                        >  2:666332/1‑140 (MQ=255)                                                           tCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGAc                                                                              >  1:721860/1‑131 (MQ=255)                                                           tCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGAc                                                                              <  2:721860/131‑1 (MQ=255)                                                           tCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAacac                                                                     >  1:432353/1‑140 (MQ=255)                                                             cGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTa                                                                   >  2:1063074/1‑140 (MQ=255)                                                               aCCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAAc                                                                 >  1:524177/1‑140 (MQ=255)                                                                 cAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAAcgc                                                               <  2:889934/140‑1 (MQ=255)                                                                         cGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCTAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCaa                                                       >  2:953575/1‑140 (MQ=255)                                                                            aGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACgg                                                    >  2:410127/1‑140 (MQ=255)                                                                               tAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCTAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTAc                                                 <  1:953575/140‑1 (MQ=255)                                                                                aaaTGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACg                                                <  1:250784/140‑1 (MQ=255)                                                                                aaaTGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACg                                                <  1:529574/140‑1 (MQ=255)                                                                                     cgcTTCCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGc                                           <  2:1056898/140‑1 (MQ=255)                                                                                     cgcTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGc                                           <  1:1063074/140‑1 (MQ=255)                                                                                       cTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTg                                         >  2:726828/1‑140 (MQ=255)                                                                                       cTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTg                                         >  1:756543/1‑140 (MQ=255)                                                                                        tACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGa                                        >  2:709946/1‑140 (MQ=255)                                                                                             gcgTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGAtcttc                                   <  1:410127/140‑1 (MQ=255)                                                                                             gcgTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGAtcttc                                   <  2:941239/140‑1 (MQ=255)                                                                                             gcgTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGAtcttc                                   <  1:420254/140‑1 (MQ=255)                                                                                               gTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGAtcttctt                                 <  1:137201/140‑1 (MQ=255)                                                                                               gTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGAtcttctt                                 <  1:971807/140‑1 (MQ=255)                                                                                                 cGCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGAtcttcttct                               <  2:756543/140‑1 (MQ=255)                                                                                                  gCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATcttcttctt                              >  2:290404/1‑140 (MQ=255)                                                                                                  gCTGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATcttcttctt                              >  1:387298/1‑140 (MQ=255)                                                                                                    tGTACGCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCACAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGt                            >  2:1137191/1‑140 (MQ=255)                                                                                                         gCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACt                       <  1:57826/140‑1 (MQ=255)                                                                                                         gCCATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACt                       <  1:709946/140‑1 (MQ=255)                                                                                                           cATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGa                     >  1:1014891/1‑140 (MQ=255)                                                                                                           cATCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGa                     >  2:369388/1‑140 (MQ=255)                                                                                                             tCACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACa                   >  2:927715/1‑140 (MQ=255)                                                                                                              cACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAg                  >  2:495731/1‑140 (MQ=255)                                                                                                              cACTTTATGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTATACTGACAg                  >  2:336152/1‑140 (MQ=255)                                                                                                                     tGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAAc           >  1:1133796/1‑140 (MQ=255)                                                                                                                     tGCACGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAAc           >  1:41942/1‑140 (MQ=255)                                                                                                                        aCGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCa        <  2:524177/140‑1 (MQ=255)                                                                                                                         cGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCaa       >  1:562464/1‑140 (MQ=255)                                                                                                                         cGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCaa       >  1:619187/1‑140 (MQ=255)                                                                                                                         cGCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCaa       >  2:1026461/1‑140 (MQ=255)                                                                                                                          gCCAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAAt      >  2:328200/1‑140 (MQ=255)                                                                                                                           ccAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATc     >  1:278714/1‑140 (MQ=255)                                                                                                                           ccAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATc     <  1:1026461/140‑1 (MQ=255)                                                                                                                           ccAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATc     <  1:927715/140‑1 (MQ=255)                                                                                                                            cAAAAGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCg    <  1:290404/140‑1 (MQ=255)                                                                                                                             aaaaGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGAc                    >  2:498006/1‑123 (MQ=255)                                                                                                                             aaaaGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGAc                    <  1:498006/123‑1 (MQ=255)                                                                                                                              aaaGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTc  <  2:619187/140‑1 (MQ=255)                                                                                                                              aaaGCGAC‑TTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTc  <  1:666332/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     GTAACACCCTGAAACCAAAGATAAATCCTGCACCATCAAATAAGGTATCCATTTTCGGTCCGACCAGAGAACCGAAGATAAATGCGCTACCCGCGTCGCTGTACGCCATCACTTTATGCACGCCAAAAGCGACTTTTTCAGCGACCCAACGCCCTGGCGGTAACCAAAGCATAATGCCGCCAATCACGACCTGTAACACTAACGCCGCGCCAACGGTACGCAGGCTGATCTTCTTCTTGTTTACTGACAGTAAAAACGCAATCGTC  >  NC_000913/2253839‑2254104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑