Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,765,794	(C)7→6	intergenic (‑18/‑124)	yfjM ← / → rnlA	CP4‑57 prophage; protein YfjM/CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,765,788	0	C	.	100.0%	345.2 / NA	82	intergenic (‑12/‑130)	yfjM/rnlA	CP4‑57 prophage; protein YfjM/CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (32/50);  total (32/50)

GGCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGA  >  NC_000913/2765650‑2765921                                                                                                                                           |                                                                                                                                       ggCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGcc                                                                                                                                       >  2:693282/1‑140 (MQ=255) ggCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGcc                                                                                                                                       >  2:566244/1‑140 (MQ=255) ggCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGcc                                                                                                                                       >  1:670931/1‑140 (MQ=255)   ctctctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGcccc                                                                                                                                     <  1:286515/140‑1 (MQ=255)    tctctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGccccc                                                                                                                                    <  1:566244/140‑1 (MQ=255)      tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGccccccg                                                                                                                                  >  2:493615/1‑139 (MQ=255)      tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGccccc                                                                                                                                    >  1:123258/1‑138 (MQ=255)      tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGccccc                                                                                                                                    <  2:123258/138‑1 (MQ=255)      tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAAAAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGcc                                                                                                                                       <  1:353090/135‑1 (MQ=255)      tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAAAAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGcc                                                                                                                                       >  2:353090/1‑135 (MQ=255)           tCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGa                                                                                                                            <  1:242902/140‑1 (MQ=255)              ttCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGa                                                                                                                         <  1:425969/140‑1 (MQ=255)                cTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGa                                                                                                                            >  2:715330/1‑135 (MQ=255)                cTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGa                                                                                                                            <  1:715330/135‑1 (MQ=255)                cTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATc                                                                                                                       <  2:306616/140‑1 (MQ=255)                cTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATc                                                                                                                       <  1:460855/140‑1 (MQ=255)                cTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATc                                                                                                                       <  1:909268/140‑1 (MQ=255)                cTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATc                                                                                                                       <  2:834241/140‑1 (MQ=255)                cTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATc                                                                                                                       <  2:991589/140‑1 (MQ=255)                 tGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATcc                                                                                                                      <  2:670931/140‑1 (MQ=255)                  gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCt                                                                                                                     >  1:1062890/1‑140 (MQ=255)                   tttCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTg                                                                                                                    >  1:623609/1‑140 (MQ=255)                    ttCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGc                                                                                                                   <  1:259821/140‑1 (MQ=255)                    ttCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGc                                                                                                                   <  1:985448/140‑1 (MQ=255)                     tCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGcc                                                                                                                  <  1:1124867/140‑1 (MQ=255)                       gACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCt                                                                                                                <  2:1132020/140‑1 (MQ=255)                        aCGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCta                                                                                                               <  2:1062890/140‑1 (MQ=255)                         cGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCtat                                                                                                              >  1:268402/1‑140 (MQ=255)                             gAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGtt                                                                                                          >  2:736649/1‑140 (MQ=255)                                  ttttCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                                     <  1:370667/140‑1 (MQ=255)                                  ttttCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                                     <  2:1084122/140‑1 (MQ=255)                                     tCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc                                                                                                  <  1:493615/140‑1 (MQ=255)                                     tCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc                                                                                                  <  1:276147/140‑1 (MQ=255)                                      cTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                                     >  2:210455/1‑136 (MQ=255)                                      cTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                                     <  1:210455/136‑1 (MQ=255)                                        tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                                     >  2:969309/1‑134 (MQ=255)                                        tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                                     <  1:969309/134‑1 (MQ=255)                                        tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcccc                                                                                                >  2:265046/1‑139 (MQ=255)                                        tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcccc                                                                                                <  1:265046/139‑1 (MQ=255)                                                ggCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAgg                                                                                       <  2:268402/140‑1 (MQ=255)                                                ggCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAgg                                                                                       <  2:623609/140‑1 (MQ=255)                                                 gCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGt                                                                                      >  2:791261/1‑140 (MQ=255)                                                             tAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAg                                                                          <  1:791261/140‑1 (MQ=255)                                                                 tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAg                                                                                        >  1:701320/1‑122 (MQ=255)                                                                 tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAg                                                                                        <  2:701320/122‑1 (MQ=255)                                                                  aTCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                                     <  1:660719/140‑1 (MQ=255)                                                                  aTCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAAt                                                                     <  1:228973/140‑1 (MQ=255)                                                                    cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCa                                                                   >  1:445610/1‑140 (MQ=255)                                                                      aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCa                                                                        <  1:780658/133‑1 (MQ=255)                                                                      aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCa                                                                        >  2:780658/1‑133 (MQ=255)                                                                      aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAAt                                                                 >  1:576039/1‑140 (MQ=255)                                                                      aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAAt                                                                 >  2:291450/1‑140 (MQ=255)                                                                      aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAAt                                                                 >  2:732649/1‑140 (MQ=255)                                                                       gTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                <  2:688553/140‑1 (MQ=255)                                                                         tgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                              >  1:473829/1‑140 (MQ=255)                                                                           ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                >  2:389640/1‑136 (MQ=255)                                                                           ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                <  1:389640/136‑1 (MQ=255)                                                                           ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACt                                                            >  1:151206/1‑140 (MQ=255)                                                                           ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACt                                                            >  2:906266/1‑140 (MQ=255)                                                                           ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACt                                                            >  2:146698/1‑140 (MQ=255)                                                                           ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACt                                                            >  2:1143627/1‑140 (MQ=255)                                                                           ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACt                                                            >  2:1056088/1‑140 (MQ=255)                                                                              ctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCa                                                         <  1:736649/140‑1 (MQ=255)                                                                                 ggTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑ggg                                                     <  1:1143627/140‑1 (MQ=255)                                                                                 ggTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑ggg                                                     <  1:693282/140‑1 (MQ=255)                                                                                 ggTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑ggg                                                     <  1:906266/140‑1 (MQ=255)                                                                                    gagaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑gggggg                                                  <  2:473829/140‑1 (MQ=255)                                                                                      gaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGta                                                <  1:134411/140‑1 (MQ=255)                                                                                          aGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGtattat                                            >  1:865833/1‑140 (MQ=255)                                                                                             aaGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGt                                         <  2:445610/140‑1 (MQ=255)                                                                                                agGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATTCACTTCA‑GGGGGGTATTATTGTaga                                      <  1:1058040/140‑1 (MQ=255)                                                                                                 ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑ggg                                                     <  1:888568/124‑1 (MQ=255)                                                                                                 ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑ggg                                                     >  2:888568/1‑124 (MQ=255)                                                                                                 ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTagag                                     >  1:270869/1‑140 (MQ=255)                                                                                                  gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGt                                    >  1:580958/1‑140 (MQ=255)                                                                                                   ttACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGtt                                   <  2:374963/140‑1 (MQ=255)                                                                                                    tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTGAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGt                                         >  2:14706/1‑133 (MQ=255)                                                                                                    tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTGAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGt                                         <  1:14706/133‑1 (MQ=255)                                                                                                       cGAAACGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTccc                               >  1:35024/1‑140 (MQ=255)                                                                                                           aCGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCata                           <  2:576039/140‑1 (MQ=255)                                                                                                            cGATGGACATCATCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCatat                          <  1:291450/140‑1 (MQ=255)                                                                                                                   catcatCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTa                   <  1:146698/140‑1 (MQ=255)                                                                                                                   catcatCCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTa                   <  2:35024/140‑1 (MQ=255)                                                                                                                    atcatcCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTAt                  <  2:151206/140‑1 (MQ=255)                                                                                                                      catcCCCCATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg                 >  2:895147/1‑140 (MQ=255)                                                                                                                         cccccATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGAt             >  2:363173/1‑140 (MQ=255)                                                                                                                         cccccATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGAt             <  2:865833/140‑1 (MQ=255)                                                                                                                            ccATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCa          <  2:724734/140‑1 (MQ=255)                                                                                                                            ccATTTTGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCa          <  1:363173/140‑1 (MQ=255)                                                                                                                                tttGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGaa      >  1:747172/1‑140 (MQ=255)                                                                                                                                 ttGTGGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGaaa     <  1:732649/140‑1 (MQ=255)                                                                                                                                    tgGACTG‑CCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGa  >  1:577791/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                       GGCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGA  >  NC_000913/2765650‑2765921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑