Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	255,174	(C)6→7	coding (543/1458 nt)	pepD ←	peptidase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	255,168	1	.	C	98.3%	200.5 / ‑3.4	59	coding (549/1458 nt)	pepD	peptidase D
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (26/32);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

GTTTGTTGGCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATA‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCAC  >  NC_000913/255029‑255302                                                                                                                                             |                                                                                                                                       gTTTGTTGGCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATa                                                                                                                                         <  1:358817/140‑1 (MQ=255) gTTTGTTGGCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATa                                                                                                                                         <  1:281666/140‑1 (MQ=255)    tgttgGCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATA‑ccc                                                                                                                                     >  1:301364/1‑140 (MQ=255)         gCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATA‑cccc                                                                                                                                    <  1:113039/136‑1 (MQ=255)         gCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATA‑cccc                                                                                                                                    >  2:113039/1‑136 (MQ=255)            ttACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATA‑cccccccgcac                                                                                                                             <  1:1011874/140‑6 (MQ=255)            ttACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATA‑cccccccgcac                                                                                                                             <  1:735726/140‑6 (MQ=255)            ttACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATA‑cccccccgcac                                                                                                                             <  1:361674/140‑6 (MQ=255)            ttACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATA‑cccc                                                                                                                                    >  1:564118/1‑133 (MQ=255)            ttACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATA‑cccc                                                                                                                                    <  2:564118/133‑1 (MQ=255)              aCCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTTGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACaa                                                                                                                            >  1:531012/1‑140 (MQ=255)                    cccAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAAcc                                                                                                                          >  1:162306/1‑136 (MQ=255)                    cccAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAAcc                                                                                                                          <  2:162306/136‑1 (MQ=255)                         cGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGAt                                                                                                                 >  1:232780/1‑140 (MQ=255)                         cGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGAt                                                                                                                 >  1:858496/1‑140 (MQ=255)                         cGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATA‑cccccccg                                                                                                                                >  2:469127/1‑122 (MQ=255)                         cGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATA‑cccccccg                                                                                                                                <  1:469127/124‑3 (MQ=255)                          gTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGAtt                                                                                                                <  2:807334/140‑1 (MQ=255)                              aTTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCAc                                                                                                            >  1:905844/1‑140 (MQ=255)                                 tCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACctt                                                                                                         <  1:1124590/140‑1 (MQ=255)                                   cccGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACcttct                                                                                                       >  1:598990/1‑140 (MQ=255)                                         ggAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTTGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCg                                                                                                 <  2:531012/140‑1 (MQ=255)                                         ggAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCg                                                                                                 <  2:867223/140‑1 (MQ=255)                                               aCCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGt                                                                                             >  1:938513/1‑138 (MQ=255)                                               aCCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGt                                                                                             <  2:938513/138‑1 (MQ=255)                                               aCCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCg                                                                                           <  2:598990/140‑1 (MQ=255)                                                 cGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGgt                                                                                         >  2:371491/1‑140 (MQ=255)                                                 cGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGgt                                                                                         <  2:426318/140‑1 (MQ=255)                                                   ccTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGgtgt                                                                                       <  2:28/140‑1 (MQ=255)                                                       tCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAAt                                                                                   <  1:823463/140‑1 (MQ=255)                                                         aGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCa                                                                                 <  2:858496/140‑1 (MQ=255)                                                              ttttAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAata                                                                            >  2:846514/1‑140 (MQ=255)                                                              ttttAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAata                                                                            >  2:679505/1‑140 (MQ=255)                                                                      ttAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTg                                                                    <  2:291711/140‑1 (MQ=255)                                                                       tAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGc                                                                   >  1:876473/1‑140 (MQ=255)                                                                        aaCTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCa                                                                  >  2:633137/1‑140 (MQ=255)                                                                               aGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGtt                                                           <  1:679505/140‑1 (MQ=255)                                                                               aGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGtt                                                           <  1:846514/140‑1 (MQ=255)                                                                                ggTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTg                                                          <  2:905844/140‑1 (MQ=255)                                                                                          ccAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGc                                                <  2:301364/140‑1 (MQ=255)                                                                                            aGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCg                                              >  2:1028721/1‑140 (MQ=255)                                                                                            aGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTACACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCg                                              <  1:1004538/140‑1 (MQ=255)                                                                                             gCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGa                                             <  2:232780/140‑1 (MQ=255)                                                                                              cTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGaa                                            >  1:777855/1‑140 (MQ=255)                                                                                               tGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAAc                                           <  2:777855/140‑1 (MQ=255)                                                                                                  aaCCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa                                        <  1:1043302/140‑1 (MQ=255)                                                                                                  aaCCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa                                        <  2:103161/140‑1 (MQ=255)                                                                                                               tCTAAATGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCgg                           <  1:374442/140‑1 (MQ=255)                                                                                                                  aaaTGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa                                        >  1:196893/1‑124 (MQ=255)                                                                                                                  aaaTGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa                                        <  2:196893/124‑1 (MQ=255)                                                                                                                   aaTGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGcttc                       <  1:371491/140‑1 (MQ=255)                                                                                                                     tGCAGGTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGcttctt                     <  1:408317/140‑1 (MQ=255)                                                                                                                         ggTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGt                 >  2:113450/1‑140 (MQ=255)                                                                                                                         ggTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGt                 <  1:1028721/140‑1 (MQ=255)                                                                                                                         ggTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGt                 >  1:833151/1‑140 (MQ=255)                                                                                                                         ggTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGt                 >  1:242984/1‑140 (MQ=255)                                                                                                                         ggTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGt                 >  2:119509/1‑140 (MQ=255)                                                                                                                          gTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTc                >  1:495881/1‑140 (MQ=255)                                                                                                                          gTTGGAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTc                >  1:145498/1‑140 (MQ=255)                                                                                                                          gTTGGAGGTGAAGTCGATA‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCa               >  1:132519/1‑140 (MQ=255)                                                                                                                             ggAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATg             >  1:324189/1‑140 (MQ=255)                                                                                                                             ggAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATg             >  2:671939/1‑140 (MQ=255)                                                                                                                             ggAGGTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATg             >  1:95977/1‑140 (MQ=255)                                                                                                                                 gTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTca         <  2:833151/140‑1 (MQ=255)                                                                                                                                 gTGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTca         <  1:671939/140‑1 (MQ=255)                                                                                                                                  tGAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTcag        >  1:1130496/1‑140 (MQ=255)                                                                                                                                   gAAGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTcagc       <  1:119509/140‑1 (MQ=255)                                                                                                                                    aaGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTcagca      <  2:242984/140‑1 (MQ=255)                                                                                                                                    aaGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTcagca      >  1:603393/1‑140 (MQ=255)                                                                                                                                     aGTCGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTcagcag     <  2:324189/140‑1 (MQ=255)                                                                                                                                        cGATACCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCAc  <  1:633137/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                       GTTTGTTGGCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATA‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGCAC  >  NC_000913/255029‑255302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑