Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,106	(C)5→6	noncoding (60/87 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,101	1	.	C	100.0%	183.0 / NA	53	noncoding (65/87 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (28/25);  total (28/25)

TTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAA‑CCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTC  >  NC_000913/4605970‑4606232                                                                                                                                     |                                                                                                                                    ttttGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAA‑ccccccacg                                                                                                                            <  1:39042/140‑5 (MQ=255)      cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGt                                                                                                                        >  2:192699/1‑140 (MQ=255)      cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGt                                                                                                                        <  2:126604/140‑1 (MQ=255)      cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGt                                                                                                                        >  2:1039184/1‑140 (MQ=255)              gTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGacac                                                                                                                >  2:668802/1‑140 (MQ=255)                 ccTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATCGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTaa                                                                                                             <  1:1083473/140‑1 (MQ=39)                      cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCt                                                                                                        >  2:22133/1‑140 (MQ=255)                             tGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                                                                                 <  1:511316/140‑1 (MQ=255)                                 ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                                                                             >  2:923458/1‑140 (MQ=255)                                 ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                                                                             >  1:92935/1‑140 (MQ=255)                                  gCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                                            <  2:92935/140‑1 (MQ=255)                                  gCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                                            >  2:236974/1‑140 (MQ=255)                                   catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGG‑AGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                                                                          >  2:837294/1‑140 (MQ=37)                                               gatAATGAATTTGAATCAGGGATGAAAAATCAAGG‑AGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                              <  1:837294/140‑1 (MQ=25)                                                   aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                                                                           >  1:149237/1‑140 (MQ=255)                                                    tGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                          >  2:902553/1‑140 (MQ=255)                                                    tGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                          >  2:864669/1‑140 (MQ=255)                                                      aaTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                                        <  1:902553/140‑1 (MQ=255)                                                       aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                                       >  1:1142165/1‑140 (MQ=255)                                                       aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                                       >  2:835905/1‑140 (MQ=255)                                                           gAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac                                                                   <  2:1142165/140‑1 (MQ=255)                                                                aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCa                                                              >  1:589715/1‑140 (MQ=255)                                                                aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCa                                                              >  2:1139931/1‑140 (MQ=255)                                                                  ggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATc                                                            >  2:133232/1‑140 (MQ=255)                                                                     tGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTa                                                         <  1:133232/140‑1 (MQ=255)                                                                      gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAc                                                        >  2:403317/1‑140 (MQ=255)                                                                                ggAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGat                                              <  1:1139931/140‑1 (MQ=255)                                                                                 gaagaaACAAGAAATGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGata                                             <  2:802980/140‑1 (MQ=21)                                                                                       aCAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCt                                       <  1:923458/140‑1 (MQ=255)                                                                                         aaGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCg                                     <  2:823824/140‑1 (MQ=255)                                                                                           gAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGtt                                   <  1:835905/140‑1 (MQ=255)                                                                                           gAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGtt                                   <  2:127533/140‑1 (MQ=255)                                                                                           gAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGtt                                   <  2:149237/140‑1 (MQ=255)                                                                                             aaGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATc                                           <  1:214728/130‑1 (MQ=255)                                                                                             aaGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATc                                           >  2:214728/1‑130 (MQ=255)                                                                                               ggAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGt                               >  1:1042163/1‑140 (MQ=255)                                                                                                gAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTg                              >  1:314576/1‑140 (MQ=255)                                                                                                  aGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCg                            <  1:864669/140‑1 (MQ=255)                                                                                                  aGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCg                            <  2:314576/140‑1 (MQ=255)                                                                                                       aGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg                       >  1:450575/1‑140 (MQ=255)                                                                                                        gATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggg                      <  1:403317/140‑1 (MQ=255)                                                                                                           aaTTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggggg                   >  1:612897/1‑140 (MQ=255)                                                                                                             ttGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGAc                 >  1:907271/1‑140 (MQ=255)                                                                                                              tGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACt                >  1:21497/1‑140 (MQ=255)                                                                                                               ggTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACtt               <  2:21497/140‑1 (MQ=255)                                                                                                                      agggggggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGCTTTGAccc         >  1:911034/2‑140 (MQ=21)                                                                                                                       gggggggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAAcccc        >  2:581940/1‑140 (MQ=255)                                                                                                                       gggggggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAAcccc        <  2:1130684/140‑1 (MQ=255)                                                                                                                        ggggggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAAccccc       <  2:907271/140‑1 (MQ=255)                                                                                                                        ggggggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGGTTGGTGCGAGGGGGGGGACTTGAAccccc       <  1:22133/140‑1 (MQ=255)                                                                                                                         gggggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCa      <  2:450575/140‑1 (MQ=255)                                                                                                                         gggggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCa      <  1:851049/140‑1 (MQ=255)                                                                                                                           gggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg    >  1:1092807/1‑140 (MQ=255)                                                                                                                             gACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTc  >  1:1006604/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                    TTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAA‑CCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTC  >  NC_000913/4605970‑4606232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑