Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,009,887	(A)6→7	coding (2044/2109 nt)	rlmL →	fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,009,881	1	.	A	100.0%	173.6 / NA	49	Q680K (CAA→AAA)	rlmL	fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (21/28);  total (21/28)

GGCGCTGATGAAAGATTTGAAACGTCTGCTGCGTGCAGGTGGGACGATCATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGC‑AAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCG  >  NC_000913/1009751‑1010014                                                                                                                                    |                                                                                                                                        ggCGCTGATGAAAGATTTGAAACGTCTGCTGCGTGCAGGTGGGACGATCATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGC‑aaaaa‑aacg                                                                                                                               <  1:424258/140‑4 (MQ=255)  gcgcTGATGAAAGATTTGAAACGTCTGCTGCGTGCAGGTGGGACGATCATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGC‑aaaaa‑aacgc                                                                                                                              >  2:65262/1‑136 (MQ=255)      tgatgaAAGATTTGAAACGTCTGCTGCGTGCAGGTGGGACGATCATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGC‑aaaaa‑aacgc                                                                                                                              <  1:611393/136‑5 (MQ=255)      tgatgaAAGATTTGAAACGTCTGCTGCGTGCAGGTGGGACGATCATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGC‑aaaaa‑aacgc                                                                                                                              >  2:611393/1‑132 (MQ=255)        atgaAAGATTTGAAACGTCTGCTGCGTGCAGGTGGGACGATCATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTccc                                                                                                                        >  1:79380/1‑140 (MQ=255)              gATTTGAAACGTCTGCTGCGTGCAGGTGGGACGATCATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGAtt                                                                                                                  <  1:1110031/140‑1 (MQ=255)                tttGAAACGTCTGCTGCGTGCAGGTGGGACGATCATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTc                                                                                                                >  1:864843/1‑140 (MQ=255)                  tGAAACGTCTGCTGCGTGCAGGTGGGACGATCATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGc                                                                                                              >  1:1054741/1‑140 (MQ=255)                  tGAAACGTCTGCTGCGTGCAGGTGGGACGATCATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGc                                                                                                              >  1:473556/1‑140 (MQ=255)                               gcGTGCAGGTGGGACGATCATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAg                                                                                                 <  2:720153/140‑1 (MQ=255)                                       gTGGGACGATCATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACaa                                                                                         <  2:898998/140‑1 (MQ=255)                                              gATCATGTTCTCAACAAACAAACGTGGCTTCCGTATGGTTCTCGGCGGCGTGGAAAAAGTGGGAGTGAAAGAAAAAAAAATTACGC‑AAAAACCC‑GCTCTCCCAGGATTTCGCCCGAAGCGGCCAGATCCACAActgctgc                                                                                  <  2:160/140‑2 (MQ=255)                                                 cATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTgctggctg                                                                               >  1:1150321/1‑140 (MQ=255)                                                  attttCTCAAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGAAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGa                                                                              <  2:1150321/137‑1 (MQ=255)                                                     ttCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTa                                                                           >  1:867213/1‑140 (MQ=255)                                                        tcGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCg                                                                        <  2:473556/140‑1 (MQ=255)                                                          gAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCg                                                                        <  1:776225/138‑1 (MQ=255)                                                          gAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCg                                                                        >  2:776225/1‑138 (MQ=255)                                                          gAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGc                                                                       >  1:1001372/1‑139 (MQ=255)                                                          gAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGc                                                                       <  2:1001372/139‑1 (MQ=255)                                                          gAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCa                                                                      >  2:1069728/1‑140 (MQ=255)                                                          gAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCa                                                                      >  2:13976/1‑140 (MQ=255)                                                          gAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCa                                                                      >  1:466917/1‑140 (MQ=255)                                                          gAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCa                                                                      >  1:930338/1‑140 (MQ=255)                                                           aacaacAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAg                                                                     >  1:697333/1‑140 (MQ=255)                                                            acaacaAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGc                                                                    <  1:13976/140‑1 (MQ=255)                                                                caaACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGa                                                                <  2:457659/140‑1 (MQ=255)                                                                 aaaCGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGaa                                                               <  2:137729/140‑1 (MQ=255)                                                                 aaaCGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGaa                                                               <  1:647753/140‑1 (MQ=255)                                                                 aaaCGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGaa                                                               <  2:609913/140‑1 (MQ=255)                                                                   aCGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAg                                                             <  2:867213/140‑1 (MQ=255)                                                                    cGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAgg                                                            <  2:930338/140‑1 (MQ=255)                                                                      tGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGa                                                                >  1:105358/1‑134 (MQ=255)                                                                      tGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGa                                                                <  2:105358/134‑1 (MQ=255)                                                                            ccGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTaa                                                    <  1:1069728/140‑1 (MQ=255)                                                                            ccGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTaa                                                    <  2:144106/140‑1 (MQ=255)                                                                                aTGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTc                                                <  2:697333/140‑1 (MQ=255)                                                                                 tGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCa                                               <  2:519924/140‑1 (MQ=255)                                                                                  ggATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCAt                                              <  1:65262/140‑1 (MQ=255)                                                                                     tctcGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTaa                                           <  2:1108865/140‑1 (MQ=255)                                                                                     tctcGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTaa                                           <  2:231270/140‑1 (MQ=255)                                                                                     tctcGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTAGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTaa                                           <  2:864843/140‑1 (MQ=255)                                                                                      ctcGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTaa                                                    >  1:1089781/1‑130 (MQ=255)                                                                                      ctcGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTaa                                                    <  2:1089781/130‑1 (MQ=255)                                                                                      ctcGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAAt                                          >  1:1039563/1‑140 (MQ=255)                                                                                       tcGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATc                                         <  1:844561/140‑1 (MQ=255)                                                                                              ccTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCa                                        >  1:1141394/1‑134 (MQ=255)                                                                                              ccTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCa                                        <  2:1141394/134‑1 (MQ=255)                                                                                                            aaCTGAAAGCCCAAGAAATTACAG‑AAAAA‑ACGGCATTCCCAGGATTACCCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTg                    <  2:288200/139‑1 (MQ=255)                                                                                                                 aaaGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGtt               >  1:83820/1‑140 (MQ=255)                                                                                                                 aaaGCACAAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGtt               >  1:526011/1‑140 (MQ=255)                                                                                                                       caAGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGTTCAGCGa         >  1:288200/1‑140 (MQ=255)                                                                                                                        aaGAAATTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGTTCAGCGAc        >  1:47268/1‑140 (MQ=255)                                                                                                                            aaTTACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGTTCAGCGACgcgc    <  2:79380/140‑1 (MQ=255)                                                                                                                              ttACGCAAAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGTTCAGCGACGcgccg  <  2:47268/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        GGCGCTGATGAAAGATTTGAAACGTCTGCTGCGTGCAGGTGGGACGATCATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGGCCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGC‑AAAAA‑AC‑GCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCCACAACTGCTGGCTGATTACCGCAGCCTGAAAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCG  >  NC_000913/1009751‑1010014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑