| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,103,745 | (T)7→8 | intergenic (‑549/‑206) | csgD ← / → csgB | DNA‑binding transcriptional dual regulator CsgD/curlin, minor subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,103,738 | 1 | . | T | 96.7% | 98.9 / ‑3.3 | 30 | intergenic (‑542/‑213) | csgD/csgB | DNA‑binding transcriptional dual regulator CsgD/curlin, minor subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (15/14); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.65e-01 | |||||||||||
TTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATA‑TTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGC > NC_000913/1103607‑1103868 | tttACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGGCATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATA‑ttttt‑ttt < 1:928323/140‑2 (MQ=255)tttACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATA‑ttttt‑ttt < 1:399972/140‑2 (MQ=255)tttACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATA‑ttttt‑ttt < 2:375949/140‑2 (MQ=255) tACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATA‑ttttt‑tttgc < 2:1069983/140‑4 (MQ=255) tACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATA‑ttttt‑tttgc < 1:955291/140‑4 (MQ=255) aGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACa < 1:119156/140‑1 (MQ=255) aGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACa < 1:517547/140‑1 (MQ=255) cTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGttt < 2:1086306/140‑1 (MQ=255) cTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGGATTTACGTGGGttt > 2:886464/1‑140 (MQ=255) tgtgCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTa < 2:525345/140‑1 (MQ=255) tgtgCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTa < 1:743002/140‑1 (MQ=255) tgCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTaa < 2:615400/139‑1 (MQ=255) tgCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTaa > 1:615400/1‑139 (MQ=255) gcAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATA‑TTTTTATTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACt < 1:973991/140‑1 (MQ=255) atCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGa > 2:870024/1‑140 (MQ=255) atCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGa > 2:220311/1‑140 (MQ=255) gcagcaATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACt > 1:1048021/1‑140 (MQ=255) agcaATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACt < 1:876547/126‑1 (MQ=255) agcaATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACt > 2:876547/1‑126 (MQ=255) agcaATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTaa > 1:760604/1‑140 (MQ=255) agcaATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTaa > 2:754770/1‑140 (MQ=255) caATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTaaaa < 2:816111/140‑1 (MQ=255) aGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGaa > 1:896090/1‑140 (MQ=255) ggCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAAAATACa > 2:216253/1‑140 (MQ=255) gCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAAAATACaa > 1:267091/1‑140 (MQ=255) aagaagAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAAAATACAAcg < 2:267091/140‑1 (MQ=255) tCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGttat > 2:268948/1‑140 (MQ=255) tCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGttat > 1:961308/1‑140 (MQ=255) tGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGttatta > 2:957877/1‑140 (MQ=255) aTAGCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCg < 1:842039/140‑1 (MQ=255) gCGGAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAAAGTACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAg < 2:1048021/140‑1 (MQ=255) ggAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGAc < 1:220311/140‑1 (MQ=255) ggAAAACGGAGATTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTCCCGCAGAc < 2:215382/140‑1 (MQ=255) gagaTTTAAAAGAAAACAAAATATTTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTc < 1:754770/140‑1 (MQ=255) aaCAAAATATTTTTT‑TTGCGTAGATAGCAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGc > 1:355272/1‑140 (MQ=255) | TTTACTGCAAAAAGACGAGGTTTGTCACGGCTTGTGCGCAAGACATATCGCAGCAATCAGCGACGGGCAAGAAGAATGACTGTCTGGTGCTTTTTGATAGCGGAAAACGGAGATTTAAAAGAAAACAAAATA‑TTTTT‑TTGCGTAGATAACAGCGTATTTACGTGGGTTTTAATACTTTGGTATGAACTAAAAAAGAAAAATACAACGCGCGGGTGAGTTATTAAAAATATTTCCGCAGACATACTTTCCATCGTAACGCAGC > NC_000913/1103607‑1103868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |