Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,698,891	(A)7→8	coding (290/1029 nt)	malI ←	DNA‑binding transcriptional repressor MalI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,698,884	1	.	A	100.0%	162.3 / NA	46	L99F (TTG→TTT)	malI	DNA‑binding transcriptional repressor MalI
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (28/18);  total (28/18)

TGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGC‑AAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGACGATCAAACCAATGACGCCGCTTTGCCCGCCGCGCAGCGCCGACGC  >  NC_000913/1698761‑1699015                                                                                                                             |                                                                                                                                    tGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGt                                                                                                                      >  2:342387/1‑140 (MQ=255)      tCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTg                                                                                                                 <  1:392147/140‑1 (MQ=255)              aGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCa                                                                                                         <  2:61143/140‑1 (MQ=255)                     cGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTc                                                                                                  >  1:1064958/1‑140 (MQ=255)                     cGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTc                                                                                                  >  2:141323/1‑140 (MQ=255)                          cTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCa                                                                                             >  2:200794/1‑140 (MQ=255)                                  aGCTCCGGCAATTACCACACCATCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGcc                                                                                     >  1:679763/1‑140 (MQ=255)                                       cGGCAATTACCACACCATCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGc                                                                                      <  1:720675/134‑1 (MQ=255)                                       cGGCAATTACCACACCATCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGc                                                                                      >  2:720675/1‑134 (MQ=255)                                                   cacCATCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTaaaa                                                                    <  2:1064958/140‑1 (MQ=255)                                                     ccATCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACg                                                                  >  1:786272/1‑140 (MQ=255)                                                       aTCGACACCCTGATTCAGTAACAGTGAAACCCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGgc                                                                <  1:258303/140‑1 (MQ=255)                                                       aTCGACACCCTGATTCAGTAACAGTGAAAACCGCTATGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGgc                                                                <  1:822012/140‑1 (MQ=255)                                                        tCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAAtt                                                                              >  2:531497/1‑125 (MQ=255)                                                        tCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAAtt                                                                              <  1:531497/125‑1 (MQ=255)                                                        tCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGgcg                                                               >  1:48660/1‑140 (MQ=255)                                                        tCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGgcg                                                               <  2:437063/140‑1 (MQ=255)                                                        tCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGgcg                                                               >  2:345587/1‑140 (MQ=255)                                                          gACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCa                                                             >  2:403263/1‑140 (MQ=255)                                                            agcccTGATTCAGTAACAGTGAAACCCGCTGTGCCAGCTGCTCCCCGTTTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGa                                                           <  2:293092/138‑1 (MQ=255)                                                              cccTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCTGCGTAAAACGGCGCAGATa                                                         >  2:534603/1‑140 (MQ=255)                                                                 tGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCAGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAAt                                                      >  1:1069951/1‑140 (MQ=255)                                                                  gATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATc                                                     >  1:1120178/1‑140 (MQ=255)                                                                   aTTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACgg                                                                 >  2:779476/1‑127 (MQ=255)                                                                   aTTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACgg                                                                 <  1:779476/127‑1 (MQ=255)                                                                      cAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACgg                                                 <  1:821598/140‑1 (MQ=255)                                                                       aGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGa                                                <  2:786272/140‑1 (MQ=255)                                                                         tAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGAct                                              >  1:293092/1‑139 (MQ=255)                                                                         tAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGACg                                              >  2:605845/1‑140 (MQ=255)                                                                         tAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGACg                                              >  2:755272/1‑140 (MQ=255)                                                                         tAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTACAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATAACGGACg                                              >  1:1031816/1‑140 (MQ=255)                                                                           aCAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGa                                                           <  2:16622/125‑1 (MQ=255)                                                                           aCAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGa                                                           >  1:16622/1‑125 (MQ=255)                                                                               tGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGACGATCaaa                                        >  2:153241/1‑140 (MQ=255)                                                                               tGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGACGATCaaa                                        >  2:306637/1‑140 (MQ=255)                                                                               tGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGACGATCaaa                                        >  1:472252/1‑140 (MQ=255)                                                                                       gCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACgg                                                 <  2:1035493/123‑1 (MQ=255)                                                                                       gCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACgg                                                 >  1:1035493/1‑123 (MQ=255)                                                                                         tgtgCCAGCTGCTCACCGTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGCCAATTCGGCGTAAAACGGCGCAGATAAATCACGGACGATCAAGCCAATGAcgc                              >  2:822961/1‑140 (MQ=255)                                                                                                          gTCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGACGATCAAACCAATGACGCCGCTTTGCCCGCCgcgc             <  1:755272/140‑1 (MQ=255)                                                                                                           tCTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGACGATCAAACCAATGACGCCGCTTTGCCCGCCGCGCa            >  2:100485/1‑140 (MQ=255)                                                                                                            cTTTACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGACGATCAAACCAATGACGCCGCTTTGCCCGCCGCGCAg           <  2:124354/140‑1 (MQ=255)                                                                                                              ttACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGACGATCAAACCAATGACGCCGCTTTGCCCGCCGCGCa            <  2:229882/137‑1 (MQ=255)                                                                                                              ttACCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGACGATCAAACCAATGACGCCGCTTTGCCCGCCGCGCa            >  1:229882/1‑137 (MQ=255)                                                                                                                aCCGCCGTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGACGATCAAACCAATGACGCCGCTTTGCCCGCCGCGCAGCGcc       <  2:1120178/140‑1 (MQ=255)                                                                                                                     cgTGAAGCAAAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGACGATCAAACCAATGACGCCGCTTTGCCCGCCGCGCAGCGCCGACGc  >  2:74783/1‑140 (MQ=255)                                                                                                                             |                                                                                                                                    TGCCATCCGTCGCAGGTCATCGCTACTTCCTGCAGCTCCGGCAATTACCACACCATCGACACCCTGATTCAGTAACAGTGAAAACCGCTGTGCCAGCTGCTCACCGTCTTTACCGCCGTGAAGC‑AAAAAAACCATCCGTCCCTGCGCTTCCAGAGCTTCCGTCAATCCGGCCGTCAATTCGGCGTAAAACGGCGCAGATAAATCACGGACGATCAAACCAATGACGCCGCTTTGCCCGCCGCGCAGCGCCGACGC  >  NC_000913/1698761‑1699015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑