Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,638,026	(C)6→7	coding (627/1179 nt)	bamB ←	outer membrane protein assembly factor BamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,638,020	1	.	C	100.0%	149.0 / NA	43	G211G (GGC→GGG)	bamB	outer membrane protein assembly factor BamB
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (22/21);  total (22/21)

CAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTG  >  NC_000913/2637881‑2638155                                                                                                                                             |                                                                                                                                         caacGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCg                                                                                                                                           <  2:873794/140‑1 (MQ=255)                 gacgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑‑ccc                                                                                                                                      >  1:1107444/1‑127 (MQ=255)                 gacgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑‑ccc                                                                                                                                      <  2:1107444/127‑1 (MQ=255)                   cgGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGc                                                                                                                        >  1:175257/1‑140 (MQ=255)                        gTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCaa                                                                                                                   >  2:1033065/1‑140 (MQ=255)                        gTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCaa                                                                                                                   <  2:1125658/140‑1 (MQ=255)                                 aCATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATTGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTg                                                                                                          <  1:301319/140‑1 (MQ=255)                                     cGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCgg                                                                                                      >  1:643422/1‑140 (MQ=255)                                       cTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGgcg                                                                                                    >  2:872033/1‑140 (MQ=255)                                            aCGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGAc                                                                                               >  2:456822/1‑140 (MQ=255)                                                  aaTTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCa                                                                                         <  1:205234/140‑1 (MQ=255)                                                    tttCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACg                                                                                       <  2:355525/140‑1 (MQ=255)                                                          tAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAaga                                                                                 <  2:398917/140‑1 (MQ=255)                                                                  gTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAg                                                                         <  2:135749/140‑1 (MQ=255)                                                                       cTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCata                                                                    <  1:485379/140‑1 (MQ=255)                                                                       cTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCata                                                                    >  1:823716/1‑140 (MQ=255)                                                                          ggAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCg                                                                 >  2:398761/1‑140 (MQ=255)                                                                               tACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGtt                                                            >  2:869025/1‑140 (MQ=255)                                                                               tACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGtt                                                            >  2:565953/1‑140 (MQ=255)                                                                                 cGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCCACGAGGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTa                                                           >  1:639359/1‑140 (MQ=255)                                                                                 cGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGc                                                                       >  1:681209/1‑127 (MQ=255)                                                                                 cGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGc                                                                       <  2:681209/127‑1 (MQ=255)                                                                                 cGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTaa                                                          <  1:872033/140‑1 (MQ=255)                                                                                     gctgcCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGt                                                      <  1:869025/140‑1 (MQ=255)                                                                                               catcTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACa                                            <  1:565953/140‑1 (MQ=255)                                                                                               catcTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACa                                            <  1:456822/140‑1 (MQ=255)                                                                                                    ggCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGcc                                       <  2:643422/140‑1 (MQ=255)                                                                                                     gCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGCCCCCCCCACGAGGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGcc                                       <  2:639359/140‑1 (MQ=255)                                                                                                      cccTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGt                                     >  2:293709/1‑140 (MQ=255)                                                                                                       ccTGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTc                                    >  2:19498/1‑140 (MQ=255)                                                                                                         tGTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAg                                  >  1:184792/1‑140 (MQ=255)                                                                                                          gTTCCATCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGc                                 >  2:375196/1‑140 (MQ=255)                                                                                                               aTCAGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGt                            >  1:1072834/1‑140 (MQ=255)                                                                                                                  aGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCa                         <  1:348062/140‑1 (MQ=255)                                                                                                                  aGCACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCa                         <  2:175257/140‑1 (MQ=255)                                                                                                                    cACTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAgc                       >  1:405353/1‑140 (MQ=255)                                                                                                                     aCTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAgcg                      >  1:491595/1‑140 (MQ=255)                                                                                                                      cTGCGCTGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGCTAACTGTCCATTTGACAGCGCCGTCAGCTCCGATCAgcgc                     >  2:423369/1‑140 (MQ=255)                                                                                                                            tGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTaa               >  2:573307/1‑140 (MQ=255)                                                                                                                            tGACGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTaa               <  1:125624/140‑1 (MQ=255)                                                                                                                              aCGCGACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACt             <  2:491595/140‑1 (MQ=255)                                                                                                                               cgcgACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTg            <  1:398761/140‑1 (MQ=255)                                                                                                                                 cgACCATTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGAc          <  1:889110/140‑1 (MQ=255)                                                                                                                                   aCCATTATCGC‑CCCCCCACGACGGCCACACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGAc          <  1:985584/138‑1 (MQ=255)                                                                                                                                   aCCATTATCGC‑CCCCCCACGACGGCCACACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGAc          >  2:985584/1‑138 (MQ=255)                                                                                                                                      aTTATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTa     <  1:423369/140‑1 (MQ=255)                                                                                                                                        tATCGC‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTG‑CAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTg  >  2:825885/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                         CAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGCGCTGACGCGACCATTATCG‑‑CCCCCCACGACGGCCGCACCAAAAGCCGTTGTCGGCGCAGACTCGCCACGCAAAGAGAGCGAAGGCATATCGAGGTTAACTGTCCATTTGACAGCGCCGTCAGCTTCGTTCAGCGCTTGTAACTGACCGTTACTG  >  NC_000913/2637881‑2638155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑