Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	11,741	T→C	M16V (ATG→GTG)	yaaI ←	DUF2541 domain‑containing protein YaaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	11,741	0	T	C	100.0%	207.2 / NA	59	M16V (ATG→GTG)	yaaI	DUF2541 domain‑containing protein YaaI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/34);  total (25/34)

TGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGATTCAGGCACG  >  NC_000913/11605‑11864                                                                                                                                         |                                                                                                                            tGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAg                                                                                                                          >  1:1128511/1‑140 (MQ=255)          gagagTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGc                                                                                                                 <  1:378809/140‑1 (MQ=255)          gagagTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGc                                                                                                                 <  2:860521/140‑1 (MQ=255)           agagTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGcc                                                                                                                <  1:998576/140‑1 (MQ=255)             agTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAg                                                                                                              >  1:451074/1‑140 (MQ=255)                  tATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTgg                                                                                                         >  1:473311/1‑140 (MQ=255)                  tATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTgg                                                                                                         >  1:76562/1‑140 (MQ=255)                  tATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTgg                                                                                                         >  2:920883/1‑140 (MQ=255)                       gTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGaa                                                                                                    >  2:333474/1‑140 (MQ=255)                         ttCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAAt                                                                                                  >  1:843565/1‑140 (MQ=255)                         ttCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAAt                                                                                                  >  1:6026/1‑140 (MQ=255)                         ttCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAAt                                                                                                  >  1:5644/1‑140 (MQ=255)                                tGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTaaaa                                                                                           >  1:867671/1‑140 (MQ=255)                                 gCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTaaaaa                                                                                          <  1:960831/140‑1 (MQ=255)                                     cAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGa                                                                                      <  2:1128511/140‑1 (MQ=255)                                     cAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGa                                                                                      <  1:1148303/140‑1 (MQ=255)                                     cAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGa                                                                                      <  2:76562/140‑1 (MQ=255)                                      aaaCAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGAt                                                                                     <  2:5937/140‑1 (MQ=255)                                        aCAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGAttt                                                                                   <  2:619989/140‑1 (MQ=255)                                            gAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATa                                                                               >  1:1147787/1‑140 (MQ=255)                                            gAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATa                                                                               >  1:675989/1‑140 (MQ=255)                                            gAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATa                                                                               >  2:201988/1‑140 (MQ=255)                                              aGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAg                                                                             <  1:960746/140‑1 (MQ=255)                                                tttCAGTGCCAGATCGTTGGTTTCGTTACGCGGCATCGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGa                                                                           <  1:1146419/140‑1 (MQ=255)                                                tttCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGa                                                                           <  2:867671/140‑1 (MQ=255)                                                 ttCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAgg                                                                            <  1:496834/138‑1 (MQ=255)                                                 ttCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAgg                                                                            >  2:496834/1‑138 (MQ=255)                                                  tCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGAtt                                                                         <  2:1147787/140‑1 (MQ=255)                                                    aGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATtct                                                                       >  2:25334/1‑140 (MQ=255)                                                     gcgcCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATtctc                                                                      <  2:334209/140‑1 (MQ=255)                                                      cgcCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTctct                                                                     >  2:951927/1‑140 (MQ=255)                                                      cgcCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTctct                                                                     <  1:237150/140‑1 (MQ=255)                                                         cAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAg                                                                  <  1:951927/140‑1 (MQ=255)                                                          aGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGt                                                                 <  2:451074/140‑1 (MQ=255)                                                            aTCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTgg                                                               >  2:469112/1‑140 (MQ=255)                                                                ttGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAag                                                           <  2:6026/140‑1 (MQ=255)                                                                     ttCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTa                                                      >  1:391264/1‑140 (MQ=255)                                                                     ttCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCCGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTa                                                      <  2:778472/140‑1 (MQ=255)                                                                      tCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAg                                                     >  1:34255/1‑140 (MQ=255)                                                                       cGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAgg                                                    <  1:333474/140‑1 (MQ=255)                                                                         ttACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAgggg                                                  >  2:456677/1‑140 (MQ=255)                                                                              cgGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGa                                             <  2:1100783/140‑1 (MQ=255)                                                                              cgGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGa                                             <  1:1099783/140‑1 (MQ=255)                                                                                 cATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATa                                          <  2:34255/140‑1 (MQ=255)                                                                                   ttGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATAcc                                        <  1:456677/140‑1 (MQ=255)                                                                                      cAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCAc                                     <  2:5644/140‑1 (MQ=255)                                                                                              ccGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTAc                             <  2:408165/140‑1 (MQ=255)                                                                                              ccGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTAc                             >  1:408165/1‑140 (MQ=255)                                                                                              ccGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTAc                             >  1:516690/1‑140 (MQ=255)                                                                                              ccGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTAc                             >  2:1141884/1‑140 (MQ=255)                                                                                              ccGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCT‑‑‑‑GTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTActgct                         >  2:700576/1‑140 (MQ=255)                                                                                                gaggagTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTActg                           <  1:201988/140‑1 (MQ=255)                                                                                                          tGGTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAaga                 <  2:516690/140‑1 (MQ=255)                                                                                                            gTCGTTTGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAgaga               <  2:473311/140‑1 (MQ=255)                                                                                                                 ttGCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGAtt          <  1:1141884/140‑1 (MQ=255)                                                                                                                   gCCTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGATTCa        <  2:395706/140‑1 (MQ=255)                                                                                                                    ccTGCGCCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGATTCAg       <  2:391264/140‑1 (MQ=255)                                                                                                                        cgcCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGATTCAGGCAc   <  1:920883/140‑1 (MQ=255)                                                                                                                         gcCGTGCAGCACAGCACCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGATTCAGGCACg  >  2:115130/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                            TGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGATTCAGGCACG  >  NC_000913/11605‑11864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑