Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,963,922	T→C	T86A (ACC→GCC)	ppdA ←	conserved protein PpdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,963,922	0	T	C	100.0%	194.4 / NA	56	T86A (ACC→GCC)	ppdA	conserved protein PpdA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/29);  total (27/29)

TTGAGTTTTTGAAGCGAATATGCCCGGCCCATGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGTATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGT  >  NC_000913/2963787‑2964054                                                                                                                                        |                                                                                                                                     ttGAGTTTTTGAAGCGAATATGCCCGGCCCATGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTg                                                                                                                                  >  2:946996/1‑140 (MQ=255)      tttttGAAGCGAATATGCCCGGCCCATGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGcccc                                                                                                                             <  1:946996/140‑1 (MQ=255)           gAAGCGAATATGCCCGGCCCATGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCcagcag                                                                                                                        <  2:9627/140‑1 (MQ=255)             aGCGAATATGCCCGGCCCATGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCg                                                                                                                      <  1:868655/140‑1 (MQ=255)               cGAATATGCCCGGCCCATGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGa                                                                                                                    <  2:928725/140‑1 (MQ=255)                  atatGCCCGGCCCATGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACt                                                                                                                 >  2:260997/1‑140 (MQ=255)                      gCCCGGCCCATGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTcaca                                                                                                             <  1:317839/140‑1 (MQ=255)                        ccGGCCCATGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAg                                                                                                           <  1:851984/140‑1 (MQ=255)                         cGGCCCATGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAgg                                                                                                          <  2:441502/140‑1 (MQ=255)                           gCCCATGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCa                                                                                                        >  2:1029978/1‑140 (MQ=255)                           gCCCATGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCa                                                                                                        >  2:813638/1‑140 (MQ=255)                             ccATGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCAcc                                                                                                      <  1:813638/140‑1 (MQ=255)                                tGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATa                                                                                                   >  2:392763/1‑140 (MQ=255)                                tGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATa                                                                                                   >  2:1036162/1‑140 (MQ=255)                                            cAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCccctccc                                                                                       <  2:332697/140‑1 (MQ=255)                                             aGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCccctccct                                                                                      >  1:218341/1‑140 (MQ=255)                                               gCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGa                                                                                    >  2:937741/1‑140 (MQ=255)                                               gCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGa                                                                                    >  2:94948/1‑140 (MQ=255)                                               gCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGa                                                                                    >  2:700209/1‑140 (MQ=255)                                               gCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGa                                                                                    >  1:934052/1‑140 (MQ=255)                                               gCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGa                                                                                    >  2:764966/1‑140 (MQ=255)                                                ccAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGAt                                                                                   <  1:260997/140‑1 (MQ=255)                                                  aaagaaagCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATaa                                                                                 <  1:700209/140‑1 (MQ=255)                                                    agaaagCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACg                                                                               <  1:392763/140‑1 (MQ=255)                                                      aaagCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCt                                                                             <  1:1029978/140‑1 (MQ=255)                                                         gcaagcGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGAt                                                                          >  1:27318/1‑140 (MQ=255)                                                         gcaagcGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGAt                                                                          >  1:499576/1‑140 (MQ=255)                                                         gcaagcGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGAt                                                                          >  2:258122/1‑140 (MQ=255)                                                                aaGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTgg                                                                   >  2:811370/1‑140 (MQ=255)                                                                 aGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGt                                                                  <  1:937741/140‑1 (MQ=255)                                                                 aGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGt                                                                  <  1:764966/140‑1 (MQ=255)                                                                  ggTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTc                                                                 <  1:831241/140‑1 (MQ=255)                                                                  ggTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTc                                                                 <  1:258122/140‑1 (MQ=255)                                                                  ggTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTc                                                                 <  2:218341/140‑1 (MQ=255)                                                                   gtgtCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTcg                                                                <  1:1252/140‑1 (MQ=255)                                                                             gCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGcc                                                      >  1:588319/1‑140 (MQ=255)                                                                              cTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGcga                                                     >  2:780356/1‑138 (MQ=255)                                                                              cTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCa                                                     >  2:590698/1‑140 (MQ=255)                                                                                    tCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGc                                               >  2:131362/1‑140 (MQ=255)                                                                                       aCTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATc                                            <  1:94948/140‑1 (MQ=255)                                                                                       aCTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATc                                            >  1:863629/1‑140 (MQ=255)                                                                                       aCTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATc                                            <  1:780356/140‑1 (MQ=255)                                                                                         ttCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCtt                                          <  1:1059382/140‑1 (MQ=255)                                                                                             ggCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACg                                      >  2:211414/1‑140 (MQ=255)                                                                                                  gCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAAt                                 <  1:590698/140‑1 (MQ=255)                                                                                                        cacaAAGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCa                           >  1:927380/1‑140 (MQ=255)                                                                                                           aaaGACCAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAAt                        <  1:1036162/140‑1 (MQ=255)                                                                                                                 cAATGGTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTccc                  >  1:984945/1‑140 (MQ=255)                                                                                                                     ggTGAACTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCgcgc              <  1:131362/140‑1 (MQ=255)                                                                                                                         aaCTGCCATGACCGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTc                    <  1:812271/130‑1 (MQ=255)                                                                                                                         aaCTGCCATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTc                    >  2:812271/1‑130 (MQ=255)                                                                                                                              ccATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCct            <  2:674815/133‑1 (MQ=255)                                                                                                                              ccATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCct            >  1:674815/1‑133 (MQ=255)                                                                                                                              ccATGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCtggctggc     >  2:869427/1‑140 (MQ=255)                                                                                                                                aTGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGCTAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCgg   <  2:1005347/140‑1 (MQ=255)                                                                                                                                 tGACAGGCATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGt  <  2:588319/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     TTGAGTTTTTGAAGCGAATATGCCCGGCCCATGCGGTATTGCGCAGGCCAAAGAAAGCAAGCGAAGGTGTCAGGTCGCTCATTTCGACTTCGGGCCAGCGTGGCACAAAGACCAATGGTGAACTGCCATGACAGGTATTGGCCCCAGCAGCGGAACTCACAAGGCACCATAACGTCCCCTCCCTGATAACGCTGATACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGT  >  NC_000913/2963787‑2964054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑