Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,966,682	A→G	Y585H (TAC→CAC)	ptsP ←	phosphoenolpyruvate‑protein phosphotransferase PtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,966,682	0	A	G	100.0%	296.8 / NA	84	Y585H (TAC→CAC)	ptsP	phosphoenolpyruvate‑protein phosphotransferase PtsP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/45);  total (39/45)

ATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCAT  >  NC_000913/2966545‑2966811                                                                                                                                          |                                                                                                                                  aTCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGAc                                                                                                                                 >  1:759003/1‑140 (MQ=255) aTCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGAc                                                                                                                                 >  2:514637/1‑140 (MQ=255)    aaCGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGAc                                                                                                                                 >  1:306379/1‑137 (MQ=255)      cGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGatc                                                                                                                            >  2:429364/1‑140 (MQ=255)         ccAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGatcatc                                                                                                                         <  1:262416/140‑1 (MQ=255)            gAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATctcc                                                                                                                      >  2:774557/1‑140 (MQ=255)             aaTGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATctcct                                                                                                                     <  1:512096/140‑1 (MQ=255)             aaTGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATctcct                                                                                                                     <  1:1006836/140‑1 (MQ=255)                       gTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCAtctc                                                                                                                       <  2:773445/128‑1 (MQ=255)                       gTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCAtctc                                                                                                                       >  1:773445/1‑128 (MQ=255)                       gTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACg                                                                                                           >  1:683871/1‑140 (MQ=255)                         cAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTc                                                                                                         >  2:1145787/1‑140 (MQ=255)                            aTCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCgg                                                                                                      <  1:245199/140‑1 (MQ=255)                                ttGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACg                                                                                                  <  1:832993/140‑1 (MQ=255)                                ttGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACg                                                                                                  >  1:1093621/1‑140 (MQ=255)                                ttGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACg                                                                                                  >  1:1068520/1‑140 (MQ=255)                                 tGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGt                                                                                                 >  2:150974/1‑140 (MQ=255)                                  ggTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGtt                                                                                                <  2:1093621/140‑1 (MQ=255)                                    tGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCa                                                                                              >  2:1116171/1‑140 (MQ=255)                                      ccAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAAt                                                                                            >  1:446592/1‑140 (MQ=255)                                      ccAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAAt                                                                                            >  1:970108/1‑140 (MQ=255)                                      ccAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAAt                                                                                            >  2:790532/1‑140 (MQ=255)                                        aaCAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCgg                                                                                                      <  2:631771/128‑1 (MQ=255)                                        aaCAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCgg                                                                                                      >  1:631771/1‑128 (MQ=255)                                          cAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAgg                                                                                        <  2:824713/140‑1 (MQ=255)                                           agagATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAggc                                                                                       >  1:351967/1‑140 (MQ=255)                                             agaTGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAggcgg                                                                                     <  1:829775/140‑1 (MQ=255)                                             agaTGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAggcgg                                                                                     <  2:13491/140‑1 (MQ=255)                                              gaTGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAggcggc                                                                                    <  1:514637/140‑1 (MQ=255)                                              gaTGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAggcggc                                                                                    <  2:703327/140‑1 (MQ=255)                                              gaTGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAggcggc                                                                                    >  1:1112026/1‑140 (MQ=255)                                               aTGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGgcggcg                                                                                   >  1:368248/1‑140 (MQ=255)                                               aTGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGgcggcg                                                                                   >  2:545636/1‑140 (MQ=255)                                                tGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGt                                                                                  <  2:368248/140‑1 (MQ=255)                                                 gAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTg                                                                                 <  1:632955/140‑1 (MQ=255)                                                   aaTCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCt                                                                               <  2:683871/140‑1 (MQ=255)                                                   aaTCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCt                                                                               <  1:187635/140‑1 (MQ=255)                                                     tCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTtc                                                                             >  2:630282/1‑140 (MQ=255)                                                       gACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTtcat                                                                           <  2:778923/140‑1 (MQ=255)                                                        aCCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGc                                                                                <  2:167981/134‑1 (MQ=255)                                                        aCCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGc                                                                                >  1:167981/1‑134 (MQ=255)                                                         cccGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATCCGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCa                                                                                          <  2:1026453/123‑1 (MQ=255)                                                         cccGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCa                                                                                          >  1:1026453/1‑123 (MQ=255)                                                         cccGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTtcatca                                                                         >  1:318476/1‑140 (MQ=255)                                                         cccGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTtcatca                                                                         >  2:114215/1‑140 (MQ=255)                                                          ccGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCaa                                                                        <  2:446592/140‑1 (MQ=255)                                                            gCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACt                                                                      <  1:150974/140‑1 (MQ=255)                                                              tttGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTc                                                                    <  2:1068520/140‑1 (MQ=255)                                                                  ccAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCg                                                                <  1:704127/140‑1 (MQ=255)                                                                  ccAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCg                                                                <  2:759003/140‑1 (MQ=255)                                                                   cAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGa                                                               <  2:970108/140‑1 (MQ=255)                                                                   cAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGa                                                               <  1:429364/140‑1 (MQ=255)                                                                              gcaTAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGAcc                                                    <  2:483686/140‑1 (MQ=255)                                                                                aTAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCAt                                                  <  2:318476/140‑1 (MQ=255)                                                                                aTAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCAt                                                  <  1:774557/140‑1 (MQ=255)                                                                                   aaTACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCgg                                               <  2:1036220/140‑1 (MQ=255)                                                                                    aTACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGc                                              >  2:341883/1‑140 (MQ=255)                                                                                        cATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACa                                          <  1:644534/140‑1 (MQ=255)                                                                                         aTTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAg                                         >  1:1133402/1‑140 (MQ=255)                                                                                         aTTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAg                                         >  2:816825/1‑140 (MQ=255)                                                                                         aTTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAg                                         <  1:630282/140‑1 (MQ=255)                                                                                         aTTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACCTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCa                                             >  1:617102/1‑136 (MQ=255)                                                                                         aTTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACCTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCa                                             <  2:617102/136‑1 (MQ=255)                                                                                             aCGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCa                                             >  2:834989/1‑132 (MQ=255)                                                                                             aCGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCa                                             <  1:834989/132‑1 (MQ=255)                                                                                             aCGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACa                                          >  1:721190/1‑135 (MQ=255)                                                                                             aCGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACa                                          <  2:721190/135‑1 (MQ=255)                                                                                                gCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTc                                  >  1:517637/1‑140 (MQ=255)                                                                                                     tCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGtt                             <  2:1133402/140‑1 (MQ=255)                                                                                                      ccAGCATGATGCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTg                            >  1:654980/1‑140 (MQ=255)                                                                                                           atgatgCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGt                       <  1:1116171/140‑1 (MQ=255)                                                                                                            tgatgCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTa                      <  1:816825/140‑1 (MQ=255)                                                                                                              atgCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGc                    >  1:602129/1‑140 (MQ=255)                                                                                                              atgCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGc                    >  1:68196/1‑140 (MQ=255)                                                                                                                gCCGATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCgg                  >  1:365002/1‑140 (MQ=255)                                                                                                                   gATACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCAt               <  2:365002/140‑1 (MQ=255)                                                                                                                    aTACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCAtt              >  2:613136/1‑140 (MQ=255)                                                                                                                     tACGTGGTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTa             <  2:1112026/140‑1 (MQ=255)                                                                                                                          ggTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACg        <  1:545636/140‑1 (MQ=255)                                                                                                                           gTTTGGGAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACgc       >  2:773301/1‑140 (MQ=255)                                                                                                                               gggAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACgcagca   <  2:68196/140‑1 (MQ=255)                                                                                                                               gggAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACgcagca   <  1:341883/140‑1 (MQ=255)                                                                                                                                ggAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCAt  <  1:114215/140‑1 (MQ=255)                                                                                                                                ggAATTTCGTGACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCATAGCGGCATTAGCACGCAGCAt  >  2:1106541/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                  ATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCAT  >  NC_000913/2966545‑2966811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑