Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,017,330	T→C	L424P (CTG→CCG)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,017,330	0	T	C	100.0%	310.9 / NA	89	L424P (CTG→CCG)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/50);  total (39/50)

AGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGA  >  NC_000913/3017193‑3017465                                                                                                                                          |                                                                                                                                        aGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCgg                                                                                                                                       >  1:274782/1‑140 (MQ=255) aGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCgg                                                                                                                                       <  1:28658/140‑1 (MQ=255)   gTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGgca                                                                                                                                     >  2:353412/1‑140 (MQ=255)    tATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGgcag                                                                                                                                    <  1:875893/140‑1 (MQ=255)       tCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGcagcag                                                                                                                                 <  2:608274/140‑1 (MQ=255)              aTTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGtt                                                                                                                          >  1:168311/1‑140 (MQ=255)              aTTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGtt                                                                                                                          <  1:249630/140‑1 (MQ=255)              aTTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGtt                                                                                                                          >  1:607522/1‑140 (MQ=255)                tACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTaa                                                                                                                        <  1:1136590/140‑1 (MQ=255)                  cTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAAcc                                                                                                                      <  2:40706/140‑1 (MQ=255)                    aTGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCAt                                                                                                                    <  2:208877/140‑1 (MQ=255)                     tGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGt                                                                                                                           >  1:88168/1‑132 (MQ=255)                     tGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGt                                                                                                                           <  2:88168/132‑1 (MQ=255)                     tGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATg                                                                                                                   >  1:42260/1‑140 (MQ=255)                     tGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATg                                                                                                                   >  2:601362/1‑140 (MQ=255)                       gCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTaa                                                                                                                        >  1:1006906/1‑133 (MQ=255)                       gCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTaa                                                                                                                        <  2:1006906/133‑1 (MQ=255)                       gCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGa                                                                                                                 >  1:407366/1‑140 (MQ=255)                       gCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGa                                                                                                                 >  2:954422/1‑140 (MQ=255)                         aaCCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTaa                                                                                                                        >  1:642849/1‑131 (MQ=255)                         aaCCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTaa                                                                                                                        <  2:642849/131‑1 (MQ=255)                         aaCCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAAt                                                                                                               >  2:345999/1‑140 (MQ=255)                         aaCCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAAt                                                                                                               >  1:103395/1‑140 (MQ=255)                          aCCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGt                                                                                                                           >  2:370491/1‑127 (MQ=255)                          aCCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGt                                                                                                                           <  1:370491/127‑1 (MQ=255)                          aCCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATa                                                                                                              <  2:556653/140‑1 (MQ=255)                          aCCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATa                                                                                                              <  1:1033535/140‑1 (MQ=255)                          aCCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATa                                                                                                              <  1:41180/140‑1 (MQ=255)                          aCCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATa                                                                                                              >  1:556653/1‑140 (MQ=255)                           ccGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATac                                                                                                             >  1:25803/1‑140 (MQ=255)                           ccGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATac                                                                                                             >  2:135054/1‑140 (MQ=255)                            cGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATaca                                                                                                            >  2:227814/1‑140 (MQ=255)                             gACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGaa                                                                                                                <  2:857651/135‑1 (MQ=255)                             gACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGaa                                                                                                                >  1:857651/1‑135 (MQ=255)                                   ctgAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGaa                                                                                                     >  1:16650/1‑140 (MQ=255)                                    tgAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGaaa                                                                                                    <  1:227814/140‑1 (MQ=255)                                      aaaCCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAaca                                                                                                  <  2:103395/140‑1 (MQ=255)                                        aCCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACt                                                                                                >  2:432169/1‑140 (MQ=255)                                         ccTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTg                                                                                               <  1:90507/140‑1 (MQ=255)                                           tGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGa                                                                                             <  2:168311/140‑1 (MQ=255)                                             gcggcTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTg                                                                                               <  2:175682/136‑1 (MQ=255)                                             gcggcTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTg                                                                                               >  1:175682/1‑136 (MQ=255)                                              cggcTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGc                                                                                          >  1:199090/1‑140 (MQ=255)                                                   aTCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCagaa                                                                                     <  1:353412/140‑1 (MQ=255)                                                   aTCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCagaa                                                                                     <  2:400151/140‑1 (MQ=255)                                                   aTCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCagaa                                                                                     >  2:89761/1‑140 (MQ=255)                                                     cTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCagaaga                                                                                   <  1:954422/140‑1 (MQ=255)                                                     cTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCagaaga                                                                                   <  2:407366/140‑1 (MQ=255)                                                      tGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAagag                                                                                  <  2:750572/140‑1 (MQ=255)                                                       gcgcTTAAGTGCCTGCATGCGCGAGCTTGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGc                                                                                 >  1:461839/1‑140 (MQ=255)                                                         gcTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGt                                                                               <  2:199090/140‑1 (MQ=255)                                                         gcTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGt                                                                               <  2:739976/140‑1 (MQ=255)                                                             aaGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAGCACTGGAAGCCAGAAGAGCGTATTg                                                                           <  2:600241/140‑1 (MQ=255)                                                              aGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGa                                                                          <  2:16650/140‑1 (MQ=255)                                                               gTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGaa                                                                         >  1:501982/1‑140 (MQ=255)                                                               gTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGaa                                                                         <  1:432169/140‑1 (MQ=255)                                                                   cTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTgg                                                                     <  1:135054/140‑1 (MQ=255)                                                                            cgAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGAcc                                                            >  2:596117/1‑140 (MQ=255)                                                                            cgAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGAcc                                                            >  1:897338/1‑140 (MQ=255)                                                                               gCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGc                                                         <  1:648285/140‑1 (MQ=255)                                                                               gCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGc                                                         <  1:596117/140‑1 (MQ=255)                                                                                    aaGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTg                                                    >  2:392619/1‑140 (MQ=255)                                                                                       gCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGAcc                                                 <  2:182497/140‑1 (MQ=255)                                                                                         tCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGa                                               >  1:1069641/1‑140 (MQ=255)                                                                                         tCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGa                                               <  2:607522/140‑1 (MQ=255)                                                                                              cGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCt                                          <  2:633478/140‑1 (MQ=255)                                                                                                   ggggACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGtt                                     <  2:461839/140‑1 (MQ=255)                                                                                                   ggggACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGtt                                     <  2:897338/140‑1 (MQ=255)                                                                                                      gACTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTACCCATGGAATACACCCAGAAACACTGGAGGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGcc                                  <  2:506741/140‑1 (MQ=255)                                                                                                       aCTTGACCATGTTGACGTCGAACAACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGccc                                 <  1:299814/140‑1 (MQ=255)                                                                                                        cTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGcccc                                <  2:982589/140‑1 (MQ=255)                                                                                                        cTTGACCATGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGcccc                                <  1:345999/140‑1 (MQ=255)                                                                                                                tGTTGACGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGGGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTa                        <  1:858335/140‑1 (MQ=255)                                                                                                                     aCGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTActgcc                   >  1:430104/1‑140 (MQ=255)                                                                                                                     aCGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACAATGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCt                              >  1:1020128/1‑129 (MQ=255)                                                                                                                     aCGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACAATGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCt                              <  2:1020128/129‑1 (MQ=255)                                                                                                                      cGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTActgcct                  >  1:288917/1‑140 (MQ=255)                                                                                                                      cGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTActgcct                  >  1:736374/1‑140 (MQ=255)                                                                                                                      cGTCGAACGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTActgcct                  >  1:446192/1‑140 (MQ=255)                                                                                                                           aaCGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCt             >  2:206221/1‑140 (MQ=255)                                                                                                                           aaCGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCt             <  1:206221/140‑1 (MQ=255)                                                                                                                           aaCGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCt             >  2:757572/1‑140 (MQ=255)                                                                                                                           aaCGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCt             >  1:596586/1‑140 (MQ=255)                                                                                                                            aCGACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTa            <  2:596586/140‑1 (MQ=255)                                                                                                                              gACTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATc          <  1:630759/140‑1 (MQ=255)                                                                                                                                cTGAACAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATcaa        <  2:42260/140‑1 (MQ=255)                                                                                                                                   aaCAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATcaa        >  2:205168/1‑137 (MQ=255)                                                                                                                                   aaCAGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATcaa        <  1:205168/137‑1 (MQ=255)                                                                                                                                      aGACCGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGa  <  1:541414/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        AGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATCAAGCAAGA  >  NC_000913/3017193‑3017465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑