Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	241,310	T→C	N665S (AAC→AGC)	fadE ←	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	241,310	0	T	C	100.0%	157.7 / NA	47	N665S (AAC→AGC)	fadE	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/22);  total (25/22)

CGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCG  >  NC_000913/241173‑241444                                                                                                                                          |                                                                                                                                       cgcgACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTc                                                                                                                                      >  1:683661/1‑140 (MQ=255)          tGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGttt                                                                                                                             <  2:679863/140‑1 (MQ=255)          tGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGttt                                                                                                                             >  1:679863/1‑140 (MQ=255)                aaCGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGt                                                                                                                       <  2:991690/140‑1 (MQ=255)                    ggTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAat                                                                                                                   >  2:541032/1‑140 (MQ=255)                    ggTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAat                                                                                                                   >  1:587917/1‑140 (MQ=255)                     gcggCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCGAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAatc                                                                                                                  >  2:246180/3‑140 (MQ=255)                        gCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAatcatc                                                                                                               <  1:245241/140‑1 (MQ=255)                         cGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATcatcc                                                                                                              <  2:587917/140‑1 (MQ=255)                             cGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCg                                                                                                          >  1:941450/1‑140 (MQ=255)                                   ttGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGtt                                                                                                    <  1:1081142/140‑1 (MQ=255)                                   ttGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGtt                                                                                                    <  2:683661/140‑1 (MQ=255)                                   ttGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGtt                                                                                                    <  1:541032/140‑1 (MQ=255)                                   ttGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGtt                                                                                                    <  1:183426/140‑1 (MQ=255)                                                gCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAg                                                                                       >  2:489158/1‑140 (MQ=255)                                                gCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAg                                                                                       >  2:319245/1‑140 (MQ=255)                                                gCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAg                                                                                       <  1:847072/140‑1 (MQ=255)                                                 ccACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGGTCAGCCTGATACAgc                                                                                      <  1:246180/140‑1 (MQ=255)                                                    cTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCa                                                                                   <  1:1055694/140‑1 (MQ=255)                                                    cTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCa                                                                                   <  1:319245/140‑1 (MQ=255)                                                             ccAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACg                                                                          <  2:826986/140‑1 (MQ=255)                                                               aGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGcc                                                                        >  1:528608/1‑140 (MQ=255)                                                               aGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGcc                                                                        >  1:118274/1‑140 (MQ=255)                                                               aGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGcc                                                                        >  1:157399/1‑140 (MQ=255)                                                                 cTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAg                                                                                       >  1:423863/1‑123 (MQ=255)                                                                 cTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAg                                                                                       <  2:423863/123‑1 (MQ=255)                                                                 cTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATc                                                                                 <  2:619218/129‑1 (MQ=255)                                                                 cTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATc                                                                                 >  1:619218/1‑129 (MQ=255)                                                                                          cGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCgg                                                         >  1:313039/1‑128 (MQ=255)                                                                                          cGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCgg                                                         <  2:313039/128‑1 (MQ=255)                                                                                            tCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCAt                                           >  1:802859/1‑140 (MQ=255)                                                                                             ccGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCAtt                                          >  2:891056/1‑140 (MQ=255)                                                                                             ccGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCAtt                                          >  1:388060/1‑140 (MQ=255)                                                                                                 tCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACgg                                      >  2:1136029/1‑140 (MQ=255)                                                                                                    ggAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCt                                   <  2:388060/140‑1 (MQ=255)                                                                                                             ccacATTCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCa                          >  2:362438/1‑140 (MQ=255)                                                                                                                  ttCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACg                     >  2:101394/1‑140 (MQ=255)                                                                                                                   tCAGCAGCCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGc                    >  1:846997/1‑140 (MQ=255)                                                                                                                     agcagcCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCtt                  >  1:214803/1‑140 (MQ=255)                                                                                                                         gcCCGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGc              <  2:118274/140‑1 (MQ=255)                                                                                                                          cccGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCa             >  1:687444/1‑140 (MQ=255)                                                                                                                          cccGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCa             <  1:616479/140‑1 (MQ=255)                                                                                                                          cccGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACAGCCTTCGTCGTCATAACGCTTCAGCa             >  1:803332/1‑140 (MQ=255)                                                                                                                           ccGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATaa                       <  2:542420/129‑1 (MQ=255)                                                                                                                           ccGGCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATaa                       >  1:542420/1‑129 (MQ=255)                                                                                                                              gCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGc         <  2:214803/140‑1 (MQ=255)                                                                                                                              gCAACCACGCGGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGc         <  1:1136029/140‑1 (MQ=255)                                                                                                                                     cgcgGCTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCg  >  1:222034/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGGCTTCATTACGGCCTTCGTCGTCATAACGCTTCAGCACGGCAGAGGCG  >  NC_000913/241173‑241444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑