Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,061,160	T→C	V104A (GTT→GCT)	scpA →	methylmalonyl‑CoA mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,061,160	0	T	C	100.0%	271.5 / NA	78	V104A (GTT→GCT)	scpA	methylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/39);  total (39/39)

TGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGTTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTCCTGTTCGACCAGATCCCGCTGGATAA  >  NC_000913/3061032‑3061295                                                                                                                                 |                                                                                                                                        tGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGAcc                                                                                                                              >  1:1063889/1‑140 (MQ=255) tGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGAcc                                                                                                                              <  1:449891/140‑1 (MQ=255) tGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGAcc                                                                                                                              <  2:580939/140‑1 (MQ=255)     ccGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAAAAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGc                                                                                                                          >  2:1026736/1‑140 (MQ=255)       gcgTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGcca                                                                                                                        <  1:743285/140‑1 (MQ=255)        cgTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGccac                                                                                                                       >  1:261301/1‑140 (MQ=255)            ccACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGAcc                                                                                                                              <  1:553066/129‑1 (MQ=255)            ccACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGAcc                                                                                                                              >  2:553066/1‑129 (MQ=255)               cTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGt                                                                                                                <  1:354552/140‑1 (MQ=255)                  tGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGc                                                                                                             >  2:456910/1‑140 (MQ=255)                    tataCCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTa                                                                                                           >  2:833402/1‑140 (MQ=255)                       aCCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGa                                                                                                        <  1:728930/140‑1 (MQ=255)                       aCCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGa                                                                                                        >  1:1097289/1‑140 (MQ=255)                        ccGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGAc                                                                                                       <  1:456910/140‑1 (MQ=255)                         cGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAGGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACt                                                                                                      >  2:763385/1‑140 (MQ=255)                         cGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACt                                                                                                      >  2:373675/1‑140 (MQ=255)                          gCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGa                                                                                                        <  2:1092701/137‑1 (MQ=255)                          gCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGa                                                                                                        >  1:1092701/1‑137 (MQ=255)                          gCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTc                                                                                                     >  2:34061/1‑140 (MQ=255)                           cccAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGc                                                                                                             <  2:828015/131‑1 (MQ=255)                           cccAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGc                                                                                                             >  1:828015/1‑131 (MQ=255)                           cccAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTcc                                                                                                    >  2:750052/1‑140 (MQ=255)                           cccAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTcc                                                                                                    >  1:793979/1‑140 (MQ=255)                            ccAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCg                                                                                                   >  2:412673/1‑140 (MQ=255)                            ccAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCg                                                                                                   >  2:215941/1‑140 (MQ=255)                            ccAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCg                                                                                                   >  1:902826/1‑140 (MQ=255)                            ccAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCg                                                                                                   >  1:1078907/1‑140 (MQ=255)                            ccAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCg                                                                                                   >  1:177304/1‑140 (MQ=255)                            ccAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTAGCTACGACTCCg                                                                                                   >  1:653995/1‑140 (MQ=255)                            ccAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGACCAACGCTTTTTATCGCCGTAACCTGGCCCCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCg                                                                                                   >  2:135553/1‑140 (MQ=255)                                   tGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAAccc                                                                                            >  2:1113511/1‑140 (MQ=255)                                     gACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCcgc                                                                                          >  1:360039/1‑140 (MQ=255)                                      aCCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCcgcg                                                                                         <  2:1078907/140‑1 (MQ=255)                                          tCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTg                                                                                     >  2:613649/1‑140 (MQ=255)                                          tCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTg                                                                                     >  2:267901/1‑140 (MQ=255)                                          tCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTACGATAACCCGCGCGTg                                                                                     >  2:1029526/1‑140 (MQ=255)                                                aGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTggcgggc                                                                               <  2:1063889/140‑1 (MQ=255)                                                    tGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACg                                                                           >  1:186839/1‑140 (MQ=255)                                                    tGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACg                                                                           >  1:454794/1‑140 (MQ=255)                                                     gCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTCACCTAGACGCCGGGCAAAAAGGTCTTTCCGCTGCGCTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGAccg                                                                          >  2:671577/1‑138 (MQ=255)                                                       tGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCg                                                                        >  1:705669/1‑140 (MQ=255)                                                        ggTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCgg                                                                       >  1:829096/1‑140 (MQ=255)                                                            tttaaaCAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCaaa                                                                   >  1:886264/5‑140 (MQ=255)                                                                aaCAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCgg                                                               <  2:902826/140‑1 (MQ=255)                                                                   aGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCg                                                            <  2:970681/140‑1 (MQ=255)                                                                   aGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCg                                                            <  1:1113511/140‑1 (MQ=255)                                                                   aGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCg                                                            <  1:39571/140‑1 (MQ=255)                                                                   aGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCg                                                            <  1:735806/140‑1 (MQ=255)                                                                     cAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTc                                                          <  1:613649/140‑1 (MQ=255)                                                                     cAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTc                                                          <  2:705669/140‑1 (MQ=255)                                                                     cAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTc                                                          <  1:267901/140‑1 (MQ=255)                                                                             tCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGa                                                  <  1:750052/140‑1 (MQ=255)                                                                             tCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGa                                                  <  1:876649/140‑1 (MQ=255)                                                                               cAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGaca                                                >  2:556528/1‑140 (MQ=255)                                                                                   gCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCg                                                            <  1:372444/124‑1 (MQ=255)                                                                                   gCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCg                                                            >  2:372444/1‑124 (MQ=255)                                                                                    cTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTg                                           <  2:1097289/140‑1 (MQ=255)                                                                                    cTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTg                                           <  2:829096/140‑1 (MQ=255)                                                                                    cTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTg                                           <  2:186839/140‑1 (MQ=255)                                                                                         tATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGa                                      <  1:556528/140‑1 (MQ=255)                                                                                                 tAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCg                                             >  2:886097/1‑125 (MQ=255)                                                                                                 tAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCg                                             <  1:886097/125‑1 (MQ=255)                                                                                                  aaCCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGt                             <  1:1026736/140‑1 (MQ=255)                                                                                                  aaCCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGt                             <  1:135553/140‑1 (MQ=255)                                                                                                   aCCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTc                            >  2:452241/1‑140 (MQ=255)                                                                                                   aCCTGGCCGCCGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTc                            >  2:934193/1‑140 (MQ=255)                                                                                                     ctagacgccgGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGTCTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTCCt                          <  1:671577/135‑1 (MQ=255)                                                                                                        gccgccGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTCCTGtt                       >  1:752722/1‑140 (MQ=255)                                                                                                        gccgccGGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTCCTGtt                       <  2:781645/140‑1 (MQ=255)                                                                                                         ccgccgGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTCCTGTTc                      >  2:288948/1‑140 (MQ=255)                                                                                                            ccgGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTCCTGTTCGAc                   <  2:388514/140‑1 (MQ=255)                                                                                                            ccgGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTCCTGTTCGAc                   <  1:934193/140‑1 (MQ=255)                                                                                                            ccgGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTCCTGTTCGAc                   <  1:452241/140‑1 (MQ=255)                                                                                                            ccgGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTCCTGTTCGAc                   <  1:288948/140‑1 (MQ=255)                                                                                                             cgGGCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTCCTGTTCGAcc                  <  1:215941/140‑1 (MQ=255)                                                                                                              gggCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTCCTGTTCGAc                   <  1:944687/138‑1 (MQ=255)                                                                                                              gggCAAAAAGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTCCTGTTCGAc                   >  2:944687/1‑138 (MQ=255)                                                                                                                    aaaGGTCTTTCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTCCTGTTCGACCAGATccc           <  1:914178/140‑1 (MQ=255)                                                                                                                             tCCGCTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTCCTGTTCGACCAGATCCCGCTGGATaa  <  1:34061/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                        TGGCCCGCGTGCCACTATGTATACCGCCCAACCGTGGACCATCCGTCAGTATGCTGGTTTTTCAACAGCAAAAGAGTCCAACGCTTTTTATCGCCGTAACCTGGCCGCCGGGCAAAAAGGTCTTTCCGTTGCGTTTGACCTTGCCACCCACCGTGGCTACGACTCCGATAACCCGCGCGTGGCGGGCGACGTCGGCAAAGCGGGCGTCGCTATCGACACCGTGGAAGATATGAAAGTCCTGTTCGACCAGATCCCGCTGGATAA  >  NC_000913/3061032‑3061295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑