Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,123,278	T→C	E176E (GAA→GAG)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,123,278	0	T	C	100.0%	259.3 / NA	73	E176E (GAA→GAG)	glcB	malate synthase G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/38);  total (35/38)

TAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGT  >  NC_000913/3123150‑3123411                                                                                                                                 |                                                                                                                                      tAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGaa                                                                                                                            <  2:775704/140‑1 (MQ=255)   aCCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAAc                                                                                                                          >  2:932694/1‑140 (MQ=255)    ccGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACg                                                                                                                         >  1:892652/1‑140 (MQ=255)      gACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACgac                                                                                                                       <  2:892652/140‑3 (MQ=255)         aaaCTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGaa                                                                                                                    >  2:322151/1‑140 (MQ=255)          aaCTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAAc                                                                                                                   >  2:133615/1‑140 (MQ=255)            cTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAAccc                                                                                                                 <  1:160928/140‑1 (MQ=255)            cTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAAccc                                                                                                                 <  1:322151/140‑1 (MQ=255)            cTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAAccc                                                                                                                 <  1:382581/140‑1 (MQ=255)               tgCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAgg                                                                                                              >  2:479612/1‑140 (MQ=255)               tgCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAgg                                                                                                              >  2:911698/1‑140 (MQ=255)                   ggAGTTCGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGAt                                                                                                          <  2:693292/140‑1 (MQ=255)                         cGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTg                                                                                                    >  2:484927/1‑140 (MQ=255)                                 ggTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACcgc                                                                                            <  1:484927/140‑1 (MQ=255)                                   tttCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAAcc                                                                                                      >  2:36029/1‑128 (MQ=255)                                   tttCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAAcc                                                                                                      <  1:36029/128‑1 (MQ=255)                                   tttCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTc                                                                                                 <  2:662504/133‑1 (MQ=255)                                   tttCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTc                                                                                                 >  1:662504/1‑133 (MQ=255)                                    ttCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGtt                                                                                         <  1:513109/140‑1 (MQ=255)                                      cTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGc                                                                                       >  1:214009/1‑140 (MQ=255)                                       tttACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCAcc                                                                                              <  2:869902/132‑1 (MQ=255)                                       tttACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCAcc                                                                                              >  1:869902/1‑132 (MQ=255)                                       tttACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCg                                                                                      <  1:133615/140‑1 (MQ=255)                                                    aaCTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCt                                                                         <  1:932694/140‑1 (MQ=255)                                                           tGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATc                                                                  >  1:245603/1‑140 (MQ=255)                                                             cGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGc                                                                <  2:1111982/140‑1 (MQ=255)                                                             cGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGc                                                                <  1:871577/140‑1 (MQ=255)                                                                   tGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTc                                                          <  2:214009/140‑1 (MQ=255)                                                                   tGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTc                                                          <  1:9192/140‑1 (MQ=255)                                                                    gTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTcc                                                         <  2:245603/140‑1 (MQ=255)                                                                     tttATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCt                                                        >  1:504971/1‑140 (MQ=255)                                                                             ccaccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgatg                                                >  2:683057/1‑140 (MQ=255)                                                                             ccaccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgatg                                                <  2:427022/140‑1 (MQ=255)                                                                              caccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgatga                                               >  2:371505/1‑140 (MQ=255)                                                                               accTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgatgat                                              >  1:446536/1‑140 (MQ=255)                                                                               accTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgatga                                               <  2:98925/139‑1 (MQ=255)                                                                               accTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgatga                                               >  1:98925/1‑139 (MQ=255)                                                                                 cTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGt                                            >  2:882502/1‑140 (MQ=255)                                                                                 cTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGt                                            >  2:760782/1‑140 (MQ=255)                                                                                   tAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCg                                          <  2:652126/140‑1 (MQ=255)                                                                                    aaaCGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGc                                         >  2:730854/1‑140 (MQ=255)                                                                                      aCGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTg                                       <  2:446536/140‑1 (MQ=255)                                                                                        gCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGcc                                     <  1:911698/140‑1 (MQ=255)                                                                                        gCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGcc                                     <  1:371505/140‑1 (MQ=255)                                                                                          caccacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGt                                   >  1:286378/1‑140 (MQ=255)                                                                                          caccacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGt                                   >  2:114990/1‑140 (MQ=255)                                                                                          caccacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGt                                   >  2:356189/1‑140 (MQ=255)                                                                                           accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGta                                  >  2:1134773/1‑140 (MQ=255)                                                                                           accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGta                                  >  2:132113/1‑140 (MQ=255)                                                                                             cacaTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtata                                <  1:1134773/140‑1 (MQ=255)                                                                                             cacaTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtata                                <  2:782227/140‑1 (MQ=255)                                                                                               caTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                              <  1:683057/140‑1 (MQ=255)                                                                                               caTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                              <  1:760782/140‑1 (MQ=255)                                                                                                  ccTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCa                           <  2:286378/140‑1 (MQ=255)                                                                                                  ccTGATAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCa                           <  2:495244/140‑1 (MQ=255)                                                                                                      aTAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTg                                       <  1:136379/124‑1 (MQ=255)                                                                                                      aTAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTg                                       >  2:136379/1‑124 (MQ=255)                                                                                                      aTAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtata                                >  2:424543/1‑131 (MQ=255)                                                                                                      aTAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtata                                <  1:424543/131‑1 (MQ=255)                                                                                                      aTAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                              <  2:276204/133‑1 (MQ=255)                                                                                                      aTAGCTGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                              >  1:276204/1‑133 (MQ=255)                                                                                                           tGCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGt                  >  1:489457/1‑140 (MQ=255)                                                                                                            gCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtata                                >  2:153995/1‑125 (MQ=255)                                                                                                            gCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtata                                <  1:153995/125‑1 (MQ=255)                                                                                                            gCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTg                 >  1:471315/1‑140 (MQ=255)                                                                                                            gCCGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTg                 >  1:313139/1‑140 (MQ=255)                                                                                                             ccGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGa                >  2:620269/1‑140 (MQ=255)                                                                                                              cGTTTTCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAg               <  1:620269/140‑1 (MQ=255)                                                                                                                  ttCCAGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGcccc           <  1:132113/140‑1 (MQ=255)                                                                                                                      aGCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCagcg       <  1:882502/140‑1 (MQ=255)                                                                                                                       gCGGTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCagcga      >  1:496560/1‑140 (MQ=255)                                                                                                                          gTAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTTCAGTGAGCCCCagcgagcg   >  2:163570/1‑140 (MQ=255)                                                                                                                           tAGAGACTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGt  <  2:496560/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      TAACCGACAAACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGT  >  NC_000913/3123150‑3123411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑