Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,137,202	T→C	T441A (ACC→GCC)	gss ←	fused glutathionylspermidine amidase/glutathionylspermidine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,137,202	0	T	C	100.0%	200.7 / NA	57	T441A (ACC→GCC)	gss	fused glutathionylspermidine amidase/glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/35);  total (22/35)

ACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGCTCCAT  >  NC_000913/3137066‑3137328                                                                                                                                         |                                                                                                                               aCCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGcttt                                                                                                                             <  1:418177/140‑1 (MQ=255)     gCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCcac                                                                                                                         <  1:837864/140‑1 (MQ=255)      cACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCcaca                                                                                                                        <  2:404736/140‑1 (MQ=255)      cACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCcaca                                                                                                                        <  1:691329/140‑1 (MQ=255)        cTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACg                                                                                                                      <  1:337478/140‑1 (MQ=255)           ccGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCcaca                                                                                                                        <  2:162356/135‑1 (MQ=255)           ccGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCcaca                                                                                                                        >  1:162356/1‑135 (MQ=255)              ggCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCcaca                                                                                                                        <  1:840212/132‑1 (MQ=255)              ggCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCcaca                                                                                                                        >  2:840212/1‑132 (MQ=255)              ggCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTa                                                                                                                <  1:406793/140‑1 (MQ=255)                cgcAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACg                                                                                                                      >  2:866354/1‑132 (MQ=255)                cgcAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACg                                                                                                                      <  1:866354/132‑1 (MQ=255)                cgcAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAAc                                                                                                              >  2:829200/1‑140 (MQ=255)                    gcaATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGt                                                                                                          <  1:556045/140‑1 (MQ=255)                    gcaATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGt                                                                                                          <  2:786939/140‑1 (MQ=255)                      aaTACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCg                                                                                                        <  2:833412/140‑1 (MQ=255)                       aTACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGc                                                                                                       <  2:684033/140‑1 (MQ=255)                       aTACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGc                                                                                                       <  1:1110232/140‑1 (MQ=255)                           gTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCtt                                                                                                   <  1:763895/140‑1 (MQ=255)                              gATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTccc                                                                                                <  2:682407/140‑1 (MQ=255)                               aTAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGc                                                                                                       >  1:903601/1‑132 (MQ=255)                               aTAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGc                                                                                                       <  2:903601/132‑1 (MQ=255)                               aTAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCtt                                                                                                   <  2:1066971/136‑1 (MQ=255)                               aTAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCtt                                                                                                   >  1:1066971/1‑136 (MQ=255)                               aTAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTcccc                                                                                               >  2:731280/1‑140 (MQ=255)                               aTAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTcccc                                                                                               >  2:1019968/1‑140 (MQ=255)                                  aGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCatc                                                                                            >  1:570152/1‑140 (MQ=255)                                      gCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCatcaatc                                                                                        <  1:550579/140‑1 (MQ=255)                                       cACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCatcaatca                                                                                       >  1:1147531/1‑140 (MQ=255)                                        aCTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAg                                                                                      <  1:872929/140‑1 (MQ=255)                                        aCTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAg                                                                                      <  1:16859/140‑1 (MQ=255)                                          ttCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGtt                                                                                    >  2:1086882/1‑140 (MQ=255)                                                   cGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGgcag                                                                           <  2:432552/140‑1 (MQ=255)                                                       tGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATc                                                                       <  1:449455/140‑1 (MQ=255)                                                           cGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcccag                                                                   <  1:731280/140‑1 (MQ=255)                                                            ggTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcccagc                                                                  <  1:1086882/140‑1 (MQ=255)                                                                     tGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCat                                                         >  1:434847/1‑140 (MQ=255)                                                                     tGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCat                                                         <  2:460225/140‑1 (MQ=255)                                                                        cACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatcca                                                      >  1:973153/1‑140 (MQ=255)                                                                           cGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatccaatc                                                   <  2:1116277/140‑1 (MQ=255)                                                                             cagcaAACTCACTGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatccaatcca                                                 >  1:750709/1‑140 (MQ=255)                                                                               gcaAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACg                                               <  1:829200/140‑1 (MQ=255)                                                                                     tCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGAt                                         >  2:633199/1‑140 (MQ=255)                                                                                          gTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATAcgcg                                    >  2:719947/1‑140 (MQ=255)                                                                                                   ttCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGc                           <  1:1019968/140‑1 (MQ=255)                                                                                                   ttCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGc                           <  1:324983/140‑1 (MQ=255)                                                                                                        gAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGcc                      <  2:434847/140‑1 (MQ=255)                                                                                                        gAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGcc                      <  1:1039396/140‑1 (MQ=255)                                                                                                             tGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTg                 >  2:393583/1‑140 (MQ=255)                                                                                                             tGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCACCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTg                 >  2:1012883/1‑140 (MQ=255)                                                                                                              gATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGc                <  1:393583/140‑1 (MQ=255)                                                                                                               aTCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCa               >  1:188250/1‑140 (MQ=255)                                                                                                                     cgcgGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCt         <  2:269612/140‑1 (MQ=255)                                                                                                                      gcgGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTg        <  2:1147531/140‑1 (MQ=255)                                                                                                                       cgGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCACCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGc       <  1:1012883/140‑1 (MQ=255)                                                                                                                         gTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGCTc     >  1:677984/1‑140 (MQ=255)                                                                                                                         gTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGCGCAGCGCCTGCTc     >  2:952977/1‑140 (MQ=255)                                                                                                                            tCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGCTCCAt  >  1:934904/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                               ACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGCTCCAT  >  NC_000913/3137066‑3137328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑