Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,165,556	T→C	T140A (ACG→GCG)	parC ←	DNA topoisomerase IV subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,165,556	0	T	C	100.0%	191.2 / NA	55	T140A (ACG→GCG)	parC	DNA topoisomerase IV subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/31);  total (24/31)

CAGGTTATGCGGTGGAATATCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGTCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATAACGGT  >  NC_000913/3165419‑3165690                                                                                                                                          |                                                                                                                                       cAGGTTATGCGGTGGAATATCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGccc                                                                                                                                      >  2:671182/1‑140 (MQ=255)      tATGCGGTGGAATATCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGc                                                                                                                                 <  1:693658/140‑1 (MQ=255)       aTGCGGTGGAATATCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGcccc                                                                                                                                     <  1:215638/135‑1 (MQ=255)       aTGCGGTGGAATATCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGcccc                                                                                                                                     >  2:215638/1‑135 (MQ=255)        tGCGGTGGAATATCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGccc                                                                                                                               >  2:948859/1‑140 (MQ=255)          cGGTGGAATATCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCa                                                                                                                             <  1:76808/140‑1 (MQ=255)             tGGAATATCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAgct                                                                                                                          >  2:196955/1‑140 (MQ=255)             tGGAATATCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAgct                                                                                                                          <  2:834988/140‑1 (MQ=255)              ggAATATCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGcccc                                                                                                                                     <  1:883401/128‑1 (MQ=255)              ggAATATCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGcccc                                                                                                                                     >  2:883401/1‑128 (MQ=255)               gAATATCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAgctcg                                                                                                                        >  2:376987/1‑140 (MQ=255)               gAATATCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAgctcg                                                                                                                        <  1:376987/140‑1 (MQ=255)                 atatCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAgctcgct                                                                                                                      <  2:981563/140‑1 (MQ=255)                 atatCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAgctcgct                                                                                                                      <  1:847703/140‑1 (MQ=255)                  tatCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAgctcgctc                                                                                                                     >  1:291639/1‑140 (MQ=255)                     cGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAAt                                                                                                                  <  1:975765/140‑1 (MQ=255)                     cGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAAt                                                                                                                  >  2:161661/1‑140 (MQ=255)                      ggTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATa                                                                                                                 <  2:955084/140‑1 (MQ=255)                       gTCGCCATGCCGACGGCAGTACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATag                                                                                                                <  1:555538/140‑1 (MQ=255)                       gTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATag                                                                                                                <  2:57296/140‑1 (MQ=255)                         cGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATagca                                                                                                              >  1:807485/1‑140 (MQ=255)                                    cGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAatat                                                                                                   >  2:135601/1‑140 (MQ=255)                                          tACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGAc                                                                                             <  2:748249/140‑1 (MQ=255)                                          tACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGAc                                                                                             <  1:988207/140‑1 (MQ=255)                                             cGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCg                                                                                               >  1:186838/1‑135 (MQ=255)                                             cGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCg                                                                                               <  2:186838/135‑1 (MQ=255)                                               gtggtgCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCg                                                                                        >  2:277133/1‑140 (MQ=255)                                               gtggtgCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCg                                                                                        >  2:1018495/1‑140 (MQ=255)                                                tggtgCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCgg                                                                                       >  2:847406/1‑140 (MQ=255)                                                  gtgCCGTTAAGCAAAATGCTTCGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGa                                                                                     >  2:896531/1‑140 (MQ=255)                                                   tgCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGAt                                                                                    <  1:1018495/140‑1 (MQ=255)                                                    gCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGAtt                                                                                   <  1:135601/140‑1 (MQ=255)                                                     ccGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAAcc                                                                                         <  1:220421/133‑1 (MQ=255)                                                     ccGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAAcc                                                                                         >  2:220421/1‑133 (MQ=255)                                                     ccGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGAtt                                                                                   <  2:127666/139‑1 (MQ=255)                                                     ccGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGAtt                                                                                   >  1:127666/1‑139 (MQ=255)                                                      cGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCg                                                                                 >  1:655454/1‑140 (MQ=255)                                                      cGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCg                                                                                 <  1:196955/140‑1 (MQ=255)                                                         tAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGgtg                                                                              <  1:101102/140‑1 (MQ=255)                                                          aaGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGAc                                                                                             >  2:264475/1‑124 (MQ=255)                                                          aaGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGAc                                                                                             <  1:264475/124‑1 (MQ=255)                                                              aaaaTGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACg                                                                         <  1:277133/140‑1 (MQ=255)                                                                        gCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCcgcg                                                               <  1:1108699/140‑1 (MQ=255)                                                                            aCGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGC‑CGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGa                                                          >  1:109279/1‑140 (MQ=255)                                                                                      aGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATc                                                 >  2:251584/1‑140 (MQ=255)                                                                                             tCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGgc                                          >  2:467329/1‑140 (MQ=255)                                                                                              cGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGgcg                                         <  1:431606/140‑1 (MQ=255)                                                                                              cGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGgcg                                         >  2:392783/1‑140 (MQ=255)                                                                                                 cTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGgcgcgc                                      <  2:576268/140‑1 (MQ=255)                                                                                                 cTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGgcgcgc                                      <  1:671182/140‑1 (MQ=255)                                                                                                 cTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGgcgcgc                                      <  1:948859/140‑1 (MQ=255)                                                                                                              ccGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCt                          <  2:830319/139‑1 (MQ=255)                                                                                                              ccGTCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCt                          >  1:830319/1‑139 (MQ=255)                                                                                                                 tCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCTGAcc                      >  2:784364/1‑140 (MQ=255)                                                                                                                 tCGAAGTTTGGCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCTGAcc                      >  2:149822/1‑140 (MQ=255)                                                                                                                           gCACCCAGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCTGACCATCAACCAGc            <  2:807485/140‑1 (MQ=255)                                                                                                                                 aGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATaa      <  1:251584/140‑1 (MQ=255)                                                                                                                                 aGTCAGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATaa      <  1:784364/140‑1 (MQ=255)                                                                                                                                     aGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATAACGGt  >  2:701678/1‑140 (MQ=255)                                                                                                                                     aGCCGCCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATAACGGt  <  2:928335/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       CAGGTTATGCGGTGGAATATCGGTCGCCATGCCGACGGCAATACCGGTGGTGCCGTTAAGCAAAATGTTTGGCAGACGGGCAGGTAGCATTTTCGGCTCCTGCAAAGTGCCGTCGAAGTTTGGCACCCAGTCAGCCGTCCCCTGCCCCAGCTCGCTCAATAGCAGCTCGGAATATTTCGACAACCGGGATTCGGTGTAACGCATTGCCGCGAACGATTTCGGATCGTCCGGCGCGCCCCAGTTCCCCTGACCATCAACCAGCGGATAACGGT  >  NC_000913/3165419‑3165690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑