Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,222,167	T→C	Y234Y (TAT→TAC)	ebgR →	DNA‑binding transcriptional repressor EbgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,222,167	0	T	C	100.0%	165.3 / NA	48	Y234Y (TAT→TAC)	ebgR	DNA‑binding transcriptional repressor EbgR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/26);  total (22/26)

GCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTT  >  NC_000913/3222030‑3222302                                                                                                                                          |                                                                                                                                        gCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTAccc                                                                                                                                       >  2:848783/1‑140 (MQ=255)        ttCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCa                                                                                                                                <  2:163010/140‑1 (MQ=255)            tGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACtgt                                                                                                                            >  1:774933/1‑140 (MQ=255)                  cGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCg                                                                                                                                      >  2:877551/1‑124 (MQ=255)                  cGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCg                                                                                                                                      <  1:877551/124‑1 (MQ=255)                   gCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAgg                                                                                                                                  <  1:110352/127‑1 (MQ=255)                   gCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAgg                                                                                                                                  >  2:110352/1‑127 (MQ=255)                   gCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGc                                                                                                                     >  2:352398/1‑140 (MQ=255)                    ccTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCt                                                                                                                    <  1:45954/140‑1 (MQ=255)                              atatGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCa                                                                                                          <  1:588339/140‑1 (MQ=255)                                       aCTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTa                                                                                                    <  1:932928/137‑1 (MQ=255)                                       aCTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTa                                                                                                    >  2:932928/1‑137 (MQ=255)                                         tGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGc                                                                                               <  1:835498/140‑1 (MQ=255)                                               aaGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTg                                                                                         <  1:690172/140‑1 (MQ=255)                                                  tGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCgg                                                                                      >  1:378406/1‑140 (MQ=255)                                                   ggTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCggg                                                                                     <  1:352398/140‑1 (MQ=255)                                                    gTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCggg                                                                                     >  2:319539/1‑139 (MQ=255)                                                    gTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCggg                                                                                     <  1:319539/139‑1 (MQ=255)                                                       cgcgAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCTGCGGGGTATGAACTGGCAAAAGTAATGCTGGCTCGGGGAACCTTGCCCGAGGCAACGTTTGTTGCTTCCCCTTTCATTGCTATCGACGTAGTGCggcgaat                                                                                 >  2:75187/1‑135 (MQ=255)                                                               ggATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc                                                                         <  2:1138959/140‑1 (MQ=255)                                                               ggATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc                                                                         <  1:1001244/140‑1 (MQ=255)                                                                  tatCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAg                                                                      <  2:378406/140‑1 (MQ=255)                                                                    tCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGc                                                                    >  1:679519/1‑140 (MQ=255)                                                                    tCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGc                                                                    >  1:880651/1‑140 (MQ=255)                                                                     cTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGcc                                                                   >  1:148178/1‑140 (MQ=255)                                                                        gcgcGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGa                                                                >  1:509414/1‑140 (MQ=255)                                                                         tgcggcgGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGaa                                                               <  1:75187/139‑1 (MQ=255)                                                                           cggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACa                                                             <  2:509414/140‑1 (MQ=255)                                                                           cggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACa                                                             <  2:148178/140‑1 (MQ=255)                                                                             gcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATc                                                           >  2:490323/1‑140 (MQ=255)                                                                              cggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc                                                                         <  2:366249/125‑1 (MQ=255)                                                                              cggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc                                                                         >  1:366249/1‑125 (MQ=255)                                                                               ggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATccc                                                         <  1:490323/140‑1 (MQ=255)                                                                                 tttttCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCcac                                                       <  1:426522/140‑1 (MQ=255)                                                                                       cAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCAGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGata                                                 <  2:807611/140‑1 (MQ=255)                                                                                               cgGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCtt                                         >  2:955569/1‑140 (MQ=255)                                                                                                           tGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                             <  1:955569/140‑1 (MQ=255)                                                                                                           tGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                             <  1:848783/140‑1 (MQ=255)                                                                                                             gCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGtt                                >  1:503215/1‑135 (MQ=255)                                                                                                             gCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGtt                                <  2:503215/135‑1 (MQ=255)                                                                                                             gCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa                           >  2:203673/1‑140 (MQ=255)                                                                                                                  aCAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCa                                     >  2:336155/1‑125 (MQ=255)                                                                                                                  aCAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCa                                     <  1:336155/125‑1 (MQ=255)                                                                                                                       tGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa                           >  2:99615/1‑130 (MQ=255)                                                                                                                       tGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa                           <  1:99615/130‑1 (MQ=255)                                                                                                                             cgcgGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcg              <  1:442544/137‑1 (MQ=255)                                                                                                                             cgcgGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcg              >  2:442544/1‑137 (MQ=255)                                                                                                                               cgGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt         >  1:807183/1‑140 (MQ=255)                                                                                                                               cgGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt         >  1:820635/1‑140 (MQ=255)                                                                                                                                      aCTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCttt  >  2:727534/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTT  >  NC_000913/3222030‑3222302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑